scholarly journals Critical Aspects on the Use of Microsatellite Markers for Assessing Genetic Identity of Crop Plant Varieties and Authenticity of their Food Derivatives

2018 ◽  
Author(s):  
Fabio Palumbo ◽  
Gianni Barcaccia
Keyword(s):  
Microsatellite Markers ◽  
Genetic Identity ◽  
Crop Plant ◽  
Critical Aspects

Genetic identity of two Indian pig types using microsatellite markers

2002 ◽  
Vol 33 (2) ◽  
pp. 158-159 ◽  
Author(s):  
R. Behl ◽  
R. Kaul ◽  
N. Sheoran ◽  
J. Behl ◽  
M. S. Tantia ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Genetic Identity

scholarly journals Identification of duplicates of cassava accessions sampled on the North Region of Brazil using microsatellite markers

2013 ◽  
Vol 43 (4) ◽  
pp. 461-467 ◽  
Author(s):  
Elisa Ferreira Moura ◽  
João Tomé de Farias Neto ◽  
José Edson Sampaio ◽  
Diehgo Tuloza da Silva ◽  
Girena Fernandes Ramalho
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Genetic Identity ◽  
Germplasm Bank ◽  
Maintenance Costs ◽  
Bank Management ◽  
The North ◽  
Germplasm Banks ◽  
Local Farmers

Duplicates are common in germplasm banks and their identification is needed to facilitate germplasm bank management and to reduce maintenance costs. The aim of this work was to identify duplicates of cassava from a germplasm bank in Eastern Amazon, which had been previously characterized both morphological and agronomically. In order to be genotyped with 15 microsatellite loci, 36 accessions were selected. These accessions were classified into 13 groups of similar morpho-agronomical characteristics. All loci were polymorphic, and 75 alleles were identified, with an average of five alleles per loci and H E = 0.66. There were determined 34 pairs of genotypes with identical multiloci profiles and the probability of genetic identity was 1.1x10-12 with probability of exclusion of 99.9999%. Among these duplicates, there are accessions sampled on different years and places, but with different names and accessions with the same name sampled in different places and years. The study identified genotypes that are grown in different places and that have been maintained over the years by local farmers.

scholarly journals Electric eels galore: microsatellite markers for population studies

2020 ◽  
Vol 18 (4) ◽  
Author(s):  
Lenice Souza-Shibatta ◽  
Dhiego G. Ferreira ◽  
Kátia F. Santos ◽  
Bruno A. Galindo ◽  
Oscar A. Shibatta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Studies ◽  
Genetic Identity ◽  
Amazon Rainforest ◽  
Cross Amplification ◽  
Electric Eel ◽  
Electric Fishes ◽  
Paternity Exclusion

Abstract Fourteen novel microsatellite loci are described and characterized in two species of electric eels, Electrophorus variiand E. voltaifrom floodplains and rivers of the Amazon rainforest. These loci are polymorphic, highly informative, and have the capacity to detect reliable levels of genetic diversity. Likewise, the high combined probability of paternity exclusion value and low combined probability of genetic identity value obtained demonstrate that the new set of loci displays suitability for paternity studies on electric eels. In addition, the cross-amplification of electric eel species implies that it may also be useful in the study of the closely related E. electricus, and to other Neotropical electric fishes (Gymnotiformes) species as tested herein.

scholarly journals Recovery, Molecular Characterization, and Ampelographic Assessment of Marginal Grapevine Germplasm from Southern Umbria (Central Italy)

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1539
Author(s):  
Alessandra Zombardo ◽  
Paolo Storchi ◽  
Paolo Valentini ◽  
Alice Ciofini ◽  
Daniele Migliaro ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Microsatellite Markers ◽  
Genetic Resources ◽  
Molecular Characterization ◽  
Plant Material ◽  
Central Italy ◽  
Genetic Identity ◽  
Food Economy

The protection of grapevine biodiversity and the safeguarding of genetic variability are certainly primary and topical objectives for wine research, especially in territories historically devoted to viticulture. To assess the autochthonous germplasm of three different districts of Southern Umbria (Central Italy), the plant material of 70 grapevines retrieved from reforested land plots or old vineyards was collected, and their genetic identity was investigated using 13 microsatellite markers (SSR). The results revealed the presence of 39 unique genotypes, divided into 24 already-known cultivars and 15 never-reported SSR profiles. Most of the grapevine accessions were then vegetatively propagated and cultivated in a vineyard collection both to be protected from extinction and to be evaluated at the ampelographic level. Overall, this work emphasizes the need for recovering the threatened genetic variability that characterizes minor neglected grapevine cultivars or biotypes of Southern Umbria germplasm, and the requirement to revalue and exploit the more valuable genetic resources to enhance the local agri-food economy.

scholarly journals Application of microsatellite markers for hybrid verification and genetic analysis of oil palm (Elaeis guineensis Jacq.)

2021 ◽  
Vol 37 (2) ◽  
pp. 1-12
Author(s):  
M.N Okoye ◽  
R Singh ◽  
M.I Uguru ◽  
C Bakoumé
Keyword(s):  
Microsatellite Markers ◽  
Seed Production ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Crop Improvement ◽  
Genetic Identity ◽  
Reliable Technique ◽  
Ample Evidence ◽  
Breeding Programmes ◽  
Routine Assessment

The legitimacy of parents and progenies used in crop improvement programmes is vital for any meaningful progress in selection. While acknowledging the shortcomings of controlled pollination in oil palm breeding and commercial seed production, the legitimacy of 20 oil palm progenies from the Nigerian Institute for Oil Palm Research (NIFOR) breeding programme was determined using 16 fluorescently-labeled microsatellite markers. The genotyping of parents and progenies was conducted by capillary electrophoresis using the ABI 3730 DNA Genetic Analyzer (Applied Biosystems, USA). Results revealed a complementary expression of the parents’ alleles in 18 out of the 20 individual progenies screened, confirming their hybridity and genetic identity. The two illegitimate progenies detected could be attributed to pollination and planting errors, respectively. A subset of three sufficiently informative loci (sMg00016, sMg00179 and sMo00102) was identified for routine quality control genotyping. The detection of illegitimate progenies provided ample evidence to substantiate the importance of assessing hybrid fidelity in breeding programmes. Furthermore, the usefulness of microsatellite markers as a reliable technique for routine assessment and unambiguous identification of oil palm crosses was established. The implications of microsatellite- based hybrid identification in oil palm varietal improvement programmes have been adequately discussed. Key words: Contamination, Genetic identity, Illegitimacy, NIFOR, Parent-Progeny relationships, seed production

scholarly journals Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean

2016 ◽  
Vol 16 (3) ◽  
pp. 234-239 ◽  
Author(s):  
Samara Rayane Pereira de Morais ◽  
Ariadna Faria Vieira ◽  
Laura Cristina da Silva Almeida ◽  
Luana Alves Rodrigues ◽  
Patrícia Guimarães Santos Melo ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Common Bean ◽  
Genetic Identity ◽  
Controlled Crosses
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