scholarly journals Metallothioneins, Saccharomyces cerevisiae, and Heavy Metals: A Biotechnology Triad?

2017 ◽  
Author(s):  
Farcasanu Ileana Cornelia ◽  
Ruta Lavinia Liliana
Keyword(s):  
Heavy Metals ◽  
Saccharomyces Cerevisiae

scholarly journals A yeast metabolome-based model for an ecotoxicological approach in the management of lignocellulosic ethanol stillage

2019 ◽  
Vol 6 (1) ◽  
pp. 180718 ◽  
Author(s):  
Luca Roscini ◽  
Lorenzo Favaro ◽  
Laura Corte ◽  
Lorenzo Cagnin ◽  
Claudia Colabella ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Saccharomyces Cerevisiae ◽  
Predictive Model ◽  
Lignocellulosic Ethanol ◽  
Bioethanol Production ◽  
Inhibitor Concentration ◽  
Promising Tool ◽  
Different Types ◽  
The Relationship ◽  
Ecotoxicological Bioassay

Lignocellulosic bioethanol production results in huge amounts of stillage, a potentially polluting by-product. Stillage, rich in heavy metals and, mainly, inhibitors, requires specific toxicity studies to be adequately managed. To this purpose, we applied an FTIR ecotoxicological bioassay to evaluate the toxicity of lignocellulosic stillage. Two weak acids and furans, most frequently found in lignocellulosic stillage, have been tested in different mixtures against three Saccharomyces cerevisiae strains. The metabolomic reaction of the test microbes and the mortality induced at various levels of inhibitor concentration showed that the strains are representative of three different types of response. Furthermore, the relationship between concentrations and FTIR synthetic stress indexes has been studied, with the aim of defining a model able to predict the concentrations of inhibitors in stillage, resulting in an optimized predictive model for all the strains. This approach represents a promising tool to support the ecotoxicological management of lignocellulosic stillage.

Synthesis, characterization, and application of Saccharomyces cerevisiae/alginate composites beads for adsorption of heavy metals

2020 ◽  
Vol 8 (4) ◽  
pp. 104009 ◽  
Author(s):  
Andreia De Rossi ◽  
Cesar V.T. Rigueto ◽  
Aline Dettmer ◽  
Luciane M. Colla ◽  
Jeferson S. Piccin
Keyword(s):  
Heavy Metals ◽  
Saccharomyces Cerevisiae

scholarly journals The Biosorption Capacity of Saccharomyces cerevisiae for Cadmium in Milk

2020 ◽  
Author(s):  
Ramoona Masoud ◽  
Kianoosh Khosravi-Darani ◽  
Anoosheh Sharifan ◽  
Gholam-Hasan Asadi ◽  
Habibollah Younesi
Keyword(s):  
Heavy Metals ◽  
Saccharomyces Cerevisiae ◽  
Metal Concentration ◽  
Inductively Coupled Plasma ◽  
Biomass Concentration ◽  
Biosorption Capacity ◽  
Inductively Coupled ◽  
Milk Samples ◽  
Icp Ms ◽  
Cadmium Absorption

This study aimed to evaluate the capacity of Saccharomyces cerevisiae for Cadmium absorption in Milk. Nowadays one of the most serious problems is heavy metals pollution. Applying microorgaisms as a novel biotechnology is so useful especially in foodstuffs. Among the biosorbents for heavy metals’ removal, Saccharomyces cerevisiae has got an increasing attention due to its popularity in food industry. In this regard, the effects of some important factors such as the initial metal concentration, biomass concentration and contact time on the biosorption capacity of Saccharomyces cerevisiae were studied. The biosorption was analyzed by the inductively coupled plasma mass spectrometer (ICP-MS). The maximum Cd bioremoval (70%) was at 80 μg/L of this metal concentration in milk samples containing 30×108 CFU Saccharomyces cerevisiae at the end of storage time (the 4th day). There were no significant differences in sensory and physicochemical properties of milk samples during storage (p < 0.05). The isotherm studies followed by two popular models; Langmuir and Freundlich and the results showed a better fit to the Langmuir isotherm. All together, the results of this project demonstrated that the approach of using this valuable yeast, could be applied for food and drinks’ detoxification and producing healthier foods.

scholarly journals Bioremediation Efficiency of Saccharomyces cerevisiae on Cadmium and Lead from Groundwater Obtained from Mining Community

2019 ◽  
Vol 2 (1) ◽  
Keyword(s):  
Heavy Metals ◽  
Saccharomyces Cerevisiae ◽  
Water Samples ◽  
Metal Tolerance ◽  
Cadmium Concentration ◽  
Heavy Metal Tolerance ◽  
Yeast Cells ◽  
Solution Ph ◽  
Effective Measure ◽  
Cadmium And Lead

Water is a vital requirement for life and it is also an effective vehicle for the transmission of diseases if contaminated. Pollution caused by heavy metals is one of the major environmental problems that are imperative to be solved. Mining of solid minerals has been identified as an entry point of heavy metals into the environment consequently polluting various components of the environment such as soil and water. Bioremediation offers a promising means to reclaim such contaminated environment in an economical and eco friendly way. The focus of this study is to evaluate the bio sorption efficiency of cadmium and lead-resistant yeast from well water samples collected from Angwan Magiro, one of the lead-contaminated villages of Niger State, North Central Nigeria. Microbial enumeration of the water samples were carried out using pours plate technique, while physicochemical parameters were done by standard methods. Tolerance ability of the yeast isolates to the heavy metals was determined by cultivating on yeast broth supplemented with synthetic solutions of 1.50 mg/L cadmium concentration and 5.50 mg/L lead concentration. Based on the result of heavy metal tolerance assay, Saccharomyces cerevisiae was then selected to determine its efficiency in bio sorption of cadmium and lead in a rotary shaker incubated at an ambient temperature for a period of 28 days. Yeast cells were separated from solutions by centrifugation and the supernatants were analyzed for residual metals in solution using Atomic Absorption Spectrophotometer (AAS). Bio sorption experiment was carried out as function of solution pH. The results of this investigation reveal that Saccharomyces cerevisiae was efficient in the removal of lead with 99.54% and cadmium with 88.24% at pH 8.20. These findings suggest that Saccharomyces cerevisiae present in heavy metalcontaminated water could be an effective measure for remediation of the ecosystem.

scholarly journals High-performance Saccharomyces cerevisiae-based biosensor for heavy metal detection

2021 ◽  
Author(s):  
Jifeng Yuan ◽  
Cong Fan ◽  
Danli Zhang ◽  
Qiwen Mo
Keyword(s):  
Heavy Metals ◽  
Saccharomyces Cerevisiae ◽  
High Performance ◽  
Colorimetric Assay ◽  
Copper Ion ◽  
Low Noise ◽  
Detection Methods ◽  
Ion Detection ◽  
Engineered Yeast ◽  
Increasing Demand

Heavy metals, i.e., Cu(II), are harmful to the environment. There is an increasing demand to develop inexpensive detection methods for heavy metals. Here, we developed a yeast biosensor with reduced-noise and improved signal output for potential on-site copper ion detection. The copper-sensing circuit was achieved by employing a secondary genetic layer to control the galactose-inducible (GAL) system in Saccharomyces cerevisiae. The reciprocal control of the Gal4 activator and Gal80 repressor under copper-responsive promoters resulted in a low-noise and ultrasensitive yeast biosensor for the copper ion detection. Furthermore, we developed a betaxanthin-based colorimetric assay, as well as 2-phenylethanol and styrene-based olfactory outputs for the copper ion detection. Notably, our engineered yeast sensor confers a narrow range switch-like behavior, which can give a “yes/no” response when coupled with betaxanthin-based visual phenotype. Taken together, we envision that the design principle established here might be applicable for developing other sensing systems for various chemical detections.

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