scholarly journals Mass Spectrometry for the Detection of Endogenous Steroids and Steroid Abuse in (Race) Horses and Human Athletes

2017 ◽  
Author(s):  
Decloedt Anneleen ◽  
Van Landschoot Anita ◽  
Vanhaecke Lynn
Keyword(s):  
Mass Spectrometry ◽  
Steroid Abuse ◽  
Race Horses ◽  
Endogenous Steroids

ENDOGENOUS NEUTRAL STEROIDS IN THE LUNG TISSUE OF EARLY AND MID-TERM HUMAN FOETUSES AND IN VITRO STUDIES ON THEIR FORMATION

1974 ◽  
Vol 75 (1) ◽  
pp. 148-158 ◽  
Author(s):  
I. Huhtaniemi
Keyword(s):  
Mass Spectrometry ◽  
Lung Tissue ◽  
Hydroxysteroid Dehydrogenase ◽  
Gas Liquid Chromatography ◽  
Wet Weight ◽  
Total Concentrations ◽  
Insight Into ◽  
Human Foetuses ◽  
Endogenous Steroids

ABSTRACT Four pools of lung tissue from 2 or 3 foetuses of 11–17 weeks' gestational age were analyzed by gas-liquid chromatography and gaschromatography-mass spectrometry for endogenous neutral steroids. All of the steroids detected were present as their mono- or disulphate conjugates. No free compounds were found. Dehydroepiandrosterone, pregnenolone and their 16α-hydroxylated derivatives were the compounds present in highest concentrations in the monosulphate fraction. The most prominent steroid in the disulphate fraction was 5-androstene-3β,17α-diol. Other compounds detected were the monosulphates of 3β,7α-dihydroxy-5-androsten-17-one, 3β,16β- dihydroxy -5- androsten-17-one, 3β,17β- dihydroxy-5-androsten-16-one, 5-androstene-3β,16β,1 7α-triol, 5-pregnene-3β,17α-diol, and 3β,17α-dihydroxy-5-pregnen-20-one and the disulphates of 5-androstene-3β,17β-diol, 3β,16α-dihydroxy-5-androsten-17-one and 5-pregnene-3β,20α-diol. The total concentrations of steroids in the lung tissue varied from 300 to 600 μg/100 g tissue wet weight. In order to gain some insight into the origin of the endogenous steroids detected, i. e. whether they were synthesized by the lung tissue or only filtered by it from the perfusing blood, minced lung tissue was incubated with dehydroepiandrosterone, pregnenolone, 3β,17α-dihydroxy-5-pregnen-20-one and with their sulphate conjugates. Evidence for the presence of the following enzymes was found: sulphokinase, 7α-hydroxylase, 16αhydroxylase, 17α-hydroxylase, 17β-hydroxysteroid dehydrogenase, 20α-hydroxysteroid dehydrogenase and C17–20 desmolase.

Use of isotope ratio mass spectrometry to differentiate between endogenous steroids and synthetic homologues in cattle: A review

2013 ◽  
Vol 772 ◽  
pp. 1-15 ◽  
Author(s):  
Geert Janssens ◽  
Dirk Courtheyn ◽  
Sven Mangelinckx ◽  
Stéphanie Prévost ◽  
Emmanuelle Bichon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Isotope Ratio ◽  
Isotope Ratio Mass Spectrometry ◽  
Endogenous Steroids

Quantification of endogenous steroids in human urine by gas chromatography mass spectrometry using a surrogate analyte approach

2010 ◽  
Vol 878 (11-12) ◽  
pp. 845-852 ◽  
Author(s):  
Reza Ahmadkhaniha ◽  
Abbas Shafiee ◽  
Noushin Rastkari ◽  
Mohammad Reza Khoshayand ◽  
Farzad Kobarfard
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Human Urine ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Endogenous Steroids
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