scholarly journals Tissue Engineering of the Anterior Cruciate Ligament and Meniscus Using Acellularized Scaffolds

10.5772/8585 ◽  
2010 ◽  
Author(s):  
Gunther H. ◽  
Thomas Tischer
Keyword(s):  
Tissue Engineering ◽  
Anterior Cruciate Ligament ◽  
Cruciate Ligament ◽  
Anterior Cruciate

REGENERATION OF ANTERIOR CRUCIATE LIGAMENT WITH SILK-BASED SCAFFOLD IN PORCINE MODEL

2015 ◽  
Vol 15 (01) ◽  
pp. 1550006 ◽  
Author(s):  
ZHENG LI ◽  
JIANKANG HE ◽  
XIANG LI ◽  
WEIGUO BIAN ◽  
WENYOU ZHANG ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Anterior Cruciate Ligament ◽  
Porcine Model ◽  
Cruciate Ligament ◽  
Silk Fibers ◽  
Bone Interface ◽  
Anterior Cruciate ◽  
Large Animals

Silk was widely investigated as a promising scaffold material in ligament tissue engineering. Although a variety of silk scaffolds were developed for the regeneration of anterior cruciate ligament (ACL) in vitro and in vivo, more investigations should be performed in large animals to translate these findings into clinical applications. The aim of this study is to evaluate the feasibility of using silk-based ACL scaffolds to regenerate damaged ACLs in porcine model. The microstructural organization, tissue regeneration as well as ligament-bone interface of silk implants were evaluated with scanning electron microscopy, micro-computerized tomography, histological and immunohistochemical staining at three and six months postoperatively. The results demonstrated that silk fibers in the ACL scaffolds organized in parallel similar with collagen fibers in native ligaments, which facilitated and guided the penetration of newly regenerated tissue into the pores among silk fibers. Collagen production especially collagen I in silk implants significantly increased from three to six months, and was gradually close to the level of native ligaments. At implant-bone interface, indirect ligament-bone insertion was observed at three months and substantial Sharpey's fibers formed at six months. The results indicated that the silk-based ACL scaffold provides a promising tissue engineering approach for ACL regeneration.

Tissue engineering of the anterior cruciate ligament: a new method using acellularized tendon allografts and autologous fibroblasts

2007 ◽  
Vol 127 (9) ◽  
pp. 735-741 ◽  
Author(s):  
Thomas Tischer ◽  
Stephan Vogt ◽  
Sebastian Aryee ◽  
Erwin Steinhauser ◽  
Christopher Adamczyk ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Anterior Cruciate Ligament ◽  
Cruciate Ligament ◽  
New Method ◽  
Autologous Fibroblasts ◽  
Anterior Cruciate

Long-term hydrolytic degradation study on polymer-based embroidered scaffolds for ligament tissue engineering

2017 ◽  
Vol 47 (6) ◽  
pp. 1305-1320 ◽  
Author(s):  
Judith Hahn ◽  
Annette Breier ◽  
Harald Brünig ◽  
Gert Heinrich
Keyword(s):  
Tissue Engineering ◽  
Anterior Cruciate Ligament ◽  
Polylactic Acid ◽  
Cruciate Ligament ◽  
Hydrolytic Degradation ◽  
Ligament Injury ◽  
Degradation Behaviour ◽  
Native Anterior Cruciate Ligament ◽  
Anterior Cruciate ◽  
Ligament Tissue Engineering

Following anterior cruciate ligament injury, a mechanically stable tissue replacement is required for knee stability and to avoid subsequent damages. Tissue engineering of the anterior cruciate ligament demands a biocompatible scaffold with a controllable degradation profile to provide mechanical support for 3 to 6 months. It has been argued that embroidered textile scaffolds made of polylactic acid and poly(lactic-co-ɛ-caprolactone) fibres are a promising approach for the ligament tissue engineering with an adapted functionalization and cell seeding strategy. Therefore, the hydrolytic degradation behaviour of embroidered scaffolds made of polylactic acid and a combination of polylactic acid and poly(lactic-co-ɛ-caprolactone) fibres was investigated under physiological conditions for 168 days. The changes in the mechanical behaviour, the molecular weights as well as the surface structures were analysed. Sufficient mechanical properties comparable to native anterior cruciate ligament tissue could be demonstrated for scaffolds made of polylactic acid fibres after 6 months under hydrolysis. These results clarify the potential of using embroidered scaffolds for ligament tissue engineering.

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