scholarly journals Disruption of Metal Homeostasis and the Pathogenesis of Prion Diseases

10.5772/67327 ◽  
2017 ◽  
Author(s):  
Masahiro Kawahara ◽  
Ken-ichiro Tanaka ◽  
Dai Mizuno
Keyword(s):  
Prion Diseases ◽  
Metal Homeostasis

Rapid Quantification of Prion Proteins

2019 ◽  
Author(s):  
Matthew Healey ◽  
Muttuswamy Sivakumaran ◽  
Mark Platt
Keyword(s):  
Prion Proteins ◽  
Prion Diseases ◽  
Sample Volume ◽  
Cellular Prion Protein ◽  
Dna Aptamer ◽  
Superparamagnetic Beads ◽  
Resistive Pulse ◽  
Large Sample Volume ◽  
Complex Sample ◽  
Neurological Conditions

<p>Prion diseases are a group of fatal transmissible neurological conditions caused by the change in conformation of the normal intrinsic cellular prion protein (PrP<sup>C</sup>) in to the highly ordered insoluble amyloid state conformer (PrP<sup>SC</sup>). We present a rapid assay using Aptamers and Resistive Pulse Sensing, RPS, to extract and quantify proteins from complex sample matrices, demonstrate with the quantification of PrP<sup>c</sup>. We functionalise the surface of superparamagnetic beads, SPBs, with a DNA aptamer. First SPB’s termed P-Beads, are used to pre-concentrate the analyte from a large sample volume. The PrP<sup>c</sup> protein is then eluted from the P-Beads before aptamer modified sensing beads, S-Beads, are added. The velocity of the S-Beads through the nanopore reveals the concentration of the PrP<sup>c</sup> protein. The process is done in under an hour and allows the detection of picomol’s of protein. The technique could be easily adopted to the mutated version of the protein and integrated into clinical workflows for the screening of blood donations and transfusions. </p>

Metabolic Aspects of Prion Diseases: An Overview

2010 ◽  
Vol 11 (10) ◽  
pp. 1207-1217
Author(s):  
Tanja Vranac ◽  
Mara Bresjanac
Keyword(s):  
Prion Diseases

From Chemical Drug to Immunotherapy: New Approaches for the Treatment of Prion Diseases

2003 ◽  
Vol 3 (2) ◽  
pp. 199-205 ◽  
Author(s):  
Veronique Perrier ◽  
Carole Crozet ◽  
Jerome Solassol ◽  
Sylvain Lehmann
Keyword(s):  
Prion Diseases ◽  
New Approaches
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