scholarly journals Carbohydrate Analysis by NIRS-Chemometrics

10.5772/67208 ◽  
2017 ◽  
Author(s):  
Mercedes G. López ◽  
Ana Sarahí García-González ◽  
Elena Franco-Robles
Keyword(s):  
Carbohydrate Analysis

Development of a multi-sugar LC-MS/MS assay using simple chemical derivatization with acetic anhydride

2016 ◽  
Vol 8 (15) ◽  
pp. 3023-3033 ◽  
Author(s):  
Hermes Licea-Perez ◽  
Venkatraman Junnotula ◽  
Sylvia Zohrabian ◽  
Molly Karlinsey
Keyword(s):  
Acetic Anhydride ◽  
Chromatographic Separation ◽  
Chemical Derivatization ◽  
Carbohydrate Analysis ◽  
Simple Chemical

Carbohydrate analysis poses many analytical challenges, in terms of extraction, chromatographic separation, and detection.

Posttranslational modifications of recombinant myotube-synthesized human factor IX

Blood ◽  
2001 ◽  
Vol 97 (1) ◽  
pp. 130-138 ◽  
Author(s):  
Valder R. Arruda ◽  
James N. Hagstrom ◽  
Jeffrey Deitch ◽  
Terry Heiman-Patterson ◽  
Rodney M. Camire ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Posttranslational Modifications ◽  
Half Life ◽  
Specific Activity ◽  
Factor Ix ◽  
Serine Phosphorylation ◽  
Hemophilia B ◽  
Tyrosine Sulfation ◽  
Aav Vector ◽  
Carbohydrate Analysis

Abstract Recent data demonstrate that the introduction into skeletal muscle of an adeno-associated viral (AAV) vector expressing blood coagulation factor IX (F.IX) can result in long-term expression of the transgene product and amelioration of the bleeding diathesis in animals with hemophilia B. These data suggest that biologically active F.IX can be synthesized in skeletal muscle. Factor IX undergoes extensive posttranslational modifications in the liver, the normal site of synthesis. In addition to affecting specific activity, these posttranslational modifications can also affect recovery, half-life in the circulation, and the immunogenicity of the protein. Before initiating a human trial of an AAV-mediated, muscle-directed approach for treating hemophilia B, a detailed biochemical analysis of F.IX synthesized in skeletal muscle was carried out. As a model system, human myotubes transduced with an AAV vector expressing F.IX was used. F.IX was purified from conditioned medium using a novel strategy designed to purify material representative of all species of rF.IX in the medium. Purified F.IX was analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), N-terminal sequence analysis, chemical γ-carboxyglutamyl analysis, carbohydrate analysis, assays for tyrosine sulfation, and serine phosphorylation, and for specific activity. Results show that myotube-synthesized F.IX has specific activity similar to that of liver-synthesized F.IX. Posttranslational modifications critical for specific activity, including removal of the signal sequence and propeptide, and γ-carboxylation of the N-terminal glutamic acid residues, are also similar, but carbohydrate analysis and assessment of tyrosine sulfation and serine phosphorylation disclose differences. In vivo experiments in mice showed that these differences affect recovery but not half-life of muscle-synthesized F.IX.

Hybrid Dynamic Coating withn-Dodecylβ-d-Maltoside and Methyl Cellulose for High-Performance Carbohydrate Analysis on Poly(methyl methacrylate) Chips

2006 ◽  
Vol 78 (5) ◽  
pp. 1452-1458 ◽  
Author(s):  
Fuquan Dang ◽  
Kazuaki Kakehi ◽  
Jingjun Cheng ◽  
Osamu Tabata ◽  
Masaya Kurokawa ◽  
...  
Keyword(s):  
Methyl Methacrylate ◽  
High Performance ◽  
Methyl Cellulose ◽  
Dynamic Coating ◽  
Carbohydrate Analysis ◽  
Poly Methyl Methacrylate

scholarly journals Carbohydrate Analysis of High Fructose Corn Syrup (HFCS) Containing Commercial Beverages

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Paulin Nadi Wahjudi ◽  
Emmelyn Hsieh ◽  
Mary E Patterson ◽  
Catherine S Mao ◽  
WN Paul Lee
Keyword(s):  
High Fructose Corn Syrup ◽  
Carbohydrate Analysis ◽  
Corn Syrup ◽  
High Fructose

Decomposition of de-inking paper sludge in agricultural soils as characterized by carbohydrate analysis

2000 ◽  
Vol 32 (11-12) ◽  
pp. 1561-1570 ◽  
Author(s):  
Martin H. Chantigny ◽  
Denis A. Angers ◽  
Chantal J. Beauchamp
Keyword(s):  
Agricultural Soils ◽  
Paper Sludge ◽  
Carbohydrate Analysis
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