scholarly journals Evaluation of Genetic Diversity in Chlorophytum borivilianum (Santp. and Fernan.) Using Molecular Markers: An Endangered Medicinal Plant

Author(s):  
Sanghamitra Samantaray ◽  
Umakanta Ngangkham
2021 ◽  
Vol 35 (1) ◽  
Author(s):  
Abbas Jorkesh ◽  
Yousef Hamidoghli ◽  
Jamalali Olfati ◽  
Habibollah Samizadeh ◽  
Davood Bakhshi

The genetic variability of Froriepia subpinata Ledeb. Bail., an endangered Iranian endemic species, has been estimated with a total of 52 accessions using 20 markers including ISSR and IRAP. The results showed the polymorphic band produced by primers was 82.3%. The best mean values of genetic diversity parameters observed in ISSRs markers, being UBC873, UBC811, and UBC873 the best primers tested. The similarity range among accessions was 34.45% to 93.3%. The cluster analysis classified the accessions into five main groups that in totally, accessions with similarity in region generally were clustered in the same group. Overall, present study could provide elementary information for formulation of conservation strategies and invaluable elementary genetic information for next breeding or designing conservation programs.


2015 ◽  
Vol 2 (1) ◽  
pp. 139
Author(s):  
Danny Laurent ◽  
Nesti F. Sianipar ◽  
Chelen _ ◽  
Listiarini _ ◽  
Ariandana Wantho

<p>Rodent tuber (Typhonium flagelliforme Lodd.) is a plant from Araceae family. The plant has high medical potential as anti-cancer agent. The information regarding Indonesian rodent tuber’s genetic diversity is not available yet. Genetic information is very important for the development of rodent tuber as medicinal plant. In this research, genetic diversity and genetic distance of three Indonesian rodent tuber’s cultivars, from Bogor, Pekalongan, and Medan, were analyzed by using RAPD molecular markers. The data obtained was analyzed by NTsys software. Out of 16 primers used in the study, the 12 primers were found to be polymorphic. There were 83 bands of DNA obtained and 31 of them were polymorphic. Dendogram analysis of the three rodent tuber cultivars showed that these cultivars were clustered into two clusters. The first cluster consists of rodent tuber Bogor and Medan. The second cluster consists of rodent tuber Pekalongan. The coefficient of similarity ranged from 0.81 to 0.87. The highest coefficient of similarity was 0.87, which was detected between rodent tuber Pekalongan and Medan. The lowest coefficient of similarity was 0.81, which was detected between rodent tuber Bogor and Pekalongan. Among these three cultivars of rodent tuber, cultivar Bogor was exclusively different.</p><p><br /><strong>Keywords</strong>: Indonesia-rodent tuber, genetic diversity, RAPD-marker</p>


2017 ◽  
Vol 3 (01) ◽  
pp. 33-39
Author(s):  
Shastri P. Shukla ◽  
Abhishek Sharma

A successful assessment of genetic diversity is consider vital for formulating conservation of the rare, endangered and threatened (RET) species. Our aim was to assess the genetic diversity in Shorea tumbuggaia Roxb. population (Dipterocarpaceae family)—a critically endangered medicinal plant of conservative interest using Random Amplified Polymorphic DNA (RAPD) technique. According to International Union for Conservation of Nature (IUCN) and Conservation Assessment and Management Planning (CAMP) S. tumbuggaia is considered as an endemic and globally endangered tree species. RAPD markers were used to estimate the genetic variability and similarity among the S. tumbuggaia. Eighteen (18) primers of OPU (1-20) series generated a total of 137 polymorphic bands out of 735 total bands (19.86% polymorphism), with an average of 40.83 amplified bands per primers and 598 bands showed monomorphic banding pattern with an average of 33.22 per primers. Genetic similarity coefficients calculated from RAPD data ranged from 0.82 to 0.95, with the highest value of 0.95 in ST10 and ST11 and the lowest value of 0.82 between accessions ST2 and ST9, ST3 and ST4, ST4 and ST10 and ST5 and ST8.


Sign in / Sign up

Export Citation Format

Share Document