scholarly journals Unique Phenotypes of Endothelial Cells in Developing Arteries: A Lesson from the Ductus Arteriosus

10.5772/66501 ◽  
2017 ◽  
Author(s):  
Norika Mengchia Liu ◽  
Susumu Minamisawa
Keyword(s):  
Endothelial Cells ◽  
Ductus Arteriosus

Ibuprofen and indomethacin differentially regulate vascular endothelial growth factor and its receptors in ductus arteriosus endothelial cells

2017 ◽  
Vol 28 (3) ◽  
pp. 432-437 ◽  
Author(s):  
Hannes Sallmon ◽  
Sandra Akanbi ◽  
Sven C. Weber ◽  
Alexander Gratopp ◽  
Cornelia Rheinländer ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Ductus Arteriosus ◽  
Growth Factor Receptor ◽  
Vascular Endothelial ◽  
Aortic Endothelial Cells ◽  
Expression Levels ◽  
Ductal Closure ◽  
Endothelial Growth Factor

AbstractBackgroundCyclooxygenase inhibitors are widely applied to facilitate ductal closure in preterm infants. The mechanisms that lead to patent ductus arteriosus closure are incompletely understood. Vascular endothelial growth factor plays pivotal roles during ductal closure and remodelling.AimThe aim of this study was to investigate the effects of ibuprofen and indomethacin on the expression of vascular endothelial growth factor and its receptors in a primary rat ductus arteriosus endothelial cell culture.MethodsProtein expression of vascular endothelial growth factor and vascular endothelial growth factor receptor 1 and 2 was confirmed in rat ductus arteriosus and aorta by immunofluorescence staining. Fetal rat endothelial cells were isolated from ductus arteriosus and aorta using immunomagnetic cell sorting and treated with ibuprofen or indomethacin. mRNA expression levels were assessed by quantitative polymerase chain reaction analysis.ResultsIn ductal endothelial cells, ibuprofen significantly induced vascular endothelial growth factor and its receptor 2, but not receptor 1, whereas indomethacin did not alter the expression levels of the vascular endothelial growth factor system. In contrast, ibuprofen significantly induced vascular endothelial growth factor and its receptors 1 and 2 in aortic endothelial cells, whereas indomethacin only induced vascular endothelial growth factor receptor 2.ConclusionOur results indicate differential effects of ibuprofen and indomethacin on the expression levels of the vascular endothelial growth factor system in ductus arteriosus endothelial cells. In addition, vessel-specific differences between ductal and aortic endothelial cells were found. Further in vivo studies are needed to elucidate the biological significance of these findings.

scholarly journals Correction: Transcription Profiles of Endothelial Cells in the Rat Ductus Arteriosus during a Perinatal Period

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
Author(s):  
Norika Mengchia Liu ◽  
Tomohiro Yokota ◽  
Shun Maekawa ◽  
Ping Lü ◽  
Inbun Tei ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ductus Arteriosus ◽  
Perinatal Period ◽  
Transcription Profiles

scholarly journals The endothelial cells of ductus arteriosus have a unique gene profile to control vascular morphology

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Norika Liu ◽  
Tomohiro Yokota ◽  
Shun Maekawa ◽  
Utako Yokoyama ◽  
Takashi Kato ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ductus Arteriosus ◽  
Gene Profile ◽  
Unique Gene ◽  
Vascular Morphology

Altered plasma proteins released from platelets and endothelial cells are associated with human patent ductus arteriosus

2018 ◽  
Vol 234 (5) ◽  
pp. 6842-6853 ◽  
Author(s):  
Hai-Tao Hou ◽  
Xi-Zhang ◽  
Jun Wang ◽  
Li-Xin Liu ◽  
Jian-Feng Zhang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Patent Ductus Arteriosus ◽  
Plasma Proteins ◽  
Ductus Arteriosus ◽  
Patent Ductus

scholarly journals Aortic intimal resident macrophages are essential for maintenance of the non-thrombogenic intravascular state

2022 ◽  
Vol 1 (1) ◽  
pp. 67-84
Author(s):  
Gloria E. Hernandez ◽  
Feiyang Ma ◽  
Guadalupe Martinez ◽  
Nadia B. Firozabadi ◽  
Jocelynda Salvador ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Flow ◽  
Ductus Arteriosus ◽  
Cell Renewal ◽  
Aortic Endothelial Cells ◽  
Anatomical Changes ◽  
Direct Cell ◽  
Patent Ductus ◽  
Tunica Intima

AbstractLeukocytes and endothelial cells frequently cooperate to resolve inflammatory events. In most cases, these interactions are transient in nature and triggered by immunological insults. Here, we report that, in areas of disturbed blood flow, aortic endothelial cells permanently and intimately associate with a population of specialized macrophages. These macrophages are recruited at birth from the closing ductus arteriosus and share the luminal surface with the endothelium, becoming interwoven in the tunica intima. Anatomical changes that affect hemodynamics, such as in patent ductus arteriosus, alter macrophage seeding to coincide with regions of disturbed flow. Aortic resident macrophages expand in situ via direct cell renewal. Induced depletion of intimal macrophages leads to thrombin-mediated endothelial cell contraction, progressive fibrin accumulation and formation of microthrombi that, once dislodged, cause blockade of vessels in several organs. Together the findings reveal that intravascular resident macrophages are essential to regulate thrombin activity and clear fibrin deposits in regions of disturbed blood flow.

scholarly journals Transcription Profiles of Endothelial Cells in the Rat Ductus Arteriosus during a Perinatal Period

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73685 ◽  
Author(s):  
Norika Mengchia Liu ◽  
Tomohiro Yokota ◽  
Shun Maekawa ◽  
Ping Lü ◽  
Inbun Tei ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ductus Arteriosus ◽  
Perinatal Period ◽  
Transcription Profiles

scholarly journals Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus

2011 ◽  
Vol 70 (3) ◽  
pp. 236-241 ◽  
Author(s):  
Sven C Weber ◽  
Alexander Gratopp ◽  
Sandra Akanbi ◽  
Cornelia Rheinlaender ◽  
Hannes Sallmon ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Ductus Arteriosus ◽  
Fetal Rat

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

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