scholarly journals Guidelines for Successful Quantitative Gene Expression in Real- Time qPCR Assays

10.5772/65850 ◽  
2016 ◽  
Author(s):  
António José Rocha ◽  
Rafael de Souza Miranda ◽  
Antonio Juscelino Sudário Sousa ◽  
André Luis Coelho da Silva
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Quantitative Gene Expression ◽  
Real Time Qpcr

scholarly journals Quantitative Gene Expression Profiles in Real Time From Expressed Sequence Tag Databases

2010 ◽  
Vol 14 (6) ◽  
pp. 321-336 ◽  
Author(s):  
VINCENT A. FUNARI ◽  
KONSTANTIN VOEVODSKI ◽  
DIMITRY LEYFER ◽  
LAURA YERKES ◽  
DONALD CRAMER ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expressed Sequence Tag ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Quantitative Gene Expression ◽  
Expressed Sequence

scholarly journals Selection and validation of reference genes for quantitative gene expression studies by real-time PCR in eggplant (Solanum melongena L)

2013 ◽  
Vol 6 (1) ◽  
pp. 312 ◽  
Author(s):  
Nagavara Gantasala ◽  
Pradeep Papolu ◽  
Prasoon Thakur ◽  
Divya Kamaraju ◽  
Rohini Sreevathsa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Solanum Melongena ◽  
Quantitative Gene Expression ◽  
Expression Studies ◽  
Gene Expression Studies

scholarly journals A Panel of Stably Expressed Reference Genes for Real-Time qPCR Gene Expression Studies of Mallards (Anas platyrhynchos)

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0149454 ◽  
Author(s):  
Joanne R. Chapman ◽  
Anu S. Helin ◽  
Michelle Wille ◽  
Clara Atterby ◽  
Josef D. Järhult ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Reference Genes ◽  
Anas Platyrhynchos ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Real Time Qpcr

scholarly journals The expressions of tooth eruption relevant genes are different in incisors and molars dental follicle cells in rat: an in vitro study

2019 ◽  
Author(s):  
Mengting He ◽  
Xiaomeng Dong ◽  
Peiqi Wang ◽  
Zichao Xiang ◽  
Jiangyue Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expression Patterns ◽  
In Vitro Study ◽  
Tooth Eruption ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Real Time Qpcr

Abstract Background The incisors and molars showed different patterns of tooth eruption in rodents and the dental follicle cells play key roles in tooth eruption. Little is known about the differences in incisors and molars dental follicle cells during tooth eruption in rodents. The purpose of this study was to investigate the differences between incisor dental follicle cells and molar dental follicle cells during tooth eruption in rat.Methods Incisor dental follicle cells and molar dental follicle cells were obtained as previously described. Immunofluorescence was used to identify the cells. Gene expression was measured by real-time qPCR and western blot.Results Compared with molar dental follicle cells, the incisor dental follicle cells showed higher expression of OPG, BMP-2 and BMP-3. The molar dental follicle cells showed higher expression of MCP-1 and RANKL.Conclusions The expression patterns of genes related to tooth eruption were different in incisors and molars dental follicle cells in rat.

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