scholarly journals A Real-Time PCR-Based Diagnostic Test for Organisms in Respiratory Tract Infection

10.5772/65740 ◽  
2016 ◽  
Author(s):  
Takashi Hirama
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Real Time ◽  
Diagnostic Test ◽  
Real Time Pcr

scholarly journals Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR

2016 ◽  
Vol 64 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Bassant Meligy ◽  
Amal Sayed ◽  
Dalia Kadry Ismail ◽  
Dina Kamal ◽  
Walaa Abdel-Latif ◽  
...  
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Real Time ◽  
Lower Respiratory Tract Infection ◽  
Real Time Pcr ◽  
Lower Respiratory Tract

scholarly journals Evaluation of a Rapid Immunochromatographic ODK0501 Assay for Detecting Streptococcus pneumoniae Antigen in Sputum Samples from Patients with Lower Respiratory Tract Infection

2009 ◽  
Vol 16 (5) ◽  
pp. 672-678 ◽  
Author(s):  
Koichi Izumikawa ◽  
Suguru Akamatsu ◽  
Akiko Kageyama ◽  
Kiyomi Okada ◽  
Yukumasa Kazuyama ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Real Time ◽  
Lower Respiratory Tract Infection ◽  
Real Time Pcr ◽  
Lower Respiratory Tract ◽  
Bacterial Culture ◽  
Antigen Test

ABSTRACT A novel, rapid, and noninvasive test (ODK0501) to detect Streptococcus pneumoniae antigen was evaluated in a Japanese multicenter study. ODK0501 uses polyclonal antibodies to detect C polysaccharide of S. pneumoniae from sputum samples by an immunochromatographic assay. The utility of ODK0501 was evaluated for 161 adult patients with lower respiratory tract infection between March 2006 and March 2007. Bacterial culture and identification, real-time PCR, and ODK0501 assays were performed on sputum samples, and the Binax Now Streptococcus pneumoniae antigen test was performed using urine samples obtained from the same patients. The performances of all tests were compared based on the results of bacterial culture and identification. The sensitivity and specificity of ODK0501 were 89.1% (49/55 samples) and 95.3% (101/106 samples), respectively. We then compared the Binax Now Streptococcus pneumoniae antigen test with ODK0501 using samples from 142 patients. The sensitivities of ODK0501 and the Binax Now S. pneumoniae antigen test were 90.0% (45/50 samples) and 62.0% (31/50 samples), respectively (P = 0.002). The relative quantity of S. pneumoniae in expectorated sputum was calculated using real-time PCR and indicated that the possibility of false-positive results for ODK0501 due to indigenous S. pneumoniae was low. The positive and negative concordance rates of ODK0501 and Binax Now were 96.8% (30/31 samples) and 21.1% (4/19 samples), respectively. Binax Now was less capable of detecting S. pneumoniae antigen among patients with underlying chronic obstructive pulmonary disease. In conclusion, ODK0501 is noninvasive, rapid, and an accurate tool for diagnosing respiratory infection caused by S. pneumoniae.

scholarly journals Development of loop-mediated isothermal amplification coupled with nanoparticle-based lateral flow biosensor assay for Mycoplasma pneumoniae detection

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yacui Wang ◽  
Yi Wang ◽  
Weiwei Jiao ◽  
Jieqiong Li ◽  
Shuting Quan ◽  
...  
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Dna Templates ◽  
Loop Mediated Isothermal Amplification

AbstractMycoplasma pneumoniae (MP) is one of the most common pathogens causing respiratory tract infection, especially for community-acquired pneumonia (CAP) in school-age children. There was considerable amount of studies on loop-mediated isothermal amplification (LAMP) assay for MP detection. However, the result interpretation of these developed LAMP assays was sophisticated and subjective. Therefore, we developed and evaluated a LAMP coupled with nanoparticle-based lateral flow biosensor (LFB) assay (LAMP-LFB) for simple, reliable, and objective identification of MP (MP-LAMP-LFB). Six primers specific to P1 gene of MP were designed, and the preferred temperature for this assay was confirmed to be 65 °C. The amplification products could be visually interpreted by LFB within 2 min. The MP-LAMP-LFB assay specifically identified DNA templates of MP, and no cross-reactivity with other pathogens was obtained. The limit of the detection for this assay was 600 fg of DNA templates in pure cultures, which was in complete accordance with colorimetric indicator detection and agarose gel electrophoresis analysis. This assay was applied to 209 oropharyngeal swab specimens collected from children with acute respiratory tract infection for clinical evaluation, and compared to real-time PCR detection. Using the LAMP-LFB and real-time PCR assay, the positive rates of MP were 47.8% and 31.6%, respectively. Results suggested that the LAMP-LFB assay displayed high sensitivity compared to real-time PCR method. In summary, LAMP-LFB assay established here was a simple, objective, and sensitive assay for MP detection, which can be widely applied in clinical settings, especially in rural areas.

Association of the CTvalues of real-time PCR of viral upper respiratory tract infection with clinical severity, Kenya

2013 ◽  
Vol 85 (5) ◽  
pp. 924-932 ◽  
Author(s):  
James A. Fuller ◽  
M. Kariuki Njenga ◽  
Godfrey Bigogo ◽  
Barrack Aura ◽  
Maurice O. Ope ◽  
...  
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Real Time ◽  
Real Time Pcr ◽  
Upper Respiratory Tract Infection ◽  
Clinical Severity ◽  
Upper Respiratory Tract ◽  
Upper Respiratory
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