Ideas and Applications Toward Sample Preparation for Food and Beverage Analysis

10.5772/65587 ◽  
2017 ◽  
Keyword(s):  
Sample Preparation ◽  
Food And Beverage ◽  
Beverage Analysis

scholarly journals Rapid Method Using Two Microbial Enzymes for Detection of l-Abrine in Food as a Marker for the Toxic Protein Abrin

2014 ◽  
Vol 81 (5) ◽  
pp. 1610-1615 ◽  
Author(s):  
Anthony G. Dodge ◽  
Kelvin Carrasquillo ◽  
Luis Rivera ◽  
Lei Xu ◽  
Lawrence P. Wackett ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Visual Detection ◽  
Food Samples ◽  
Content Type ◽  
Toxic Protein ◽  
Beverage Samples ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Complex Sample ◽  
Food And Beverage

ABSTRACTAbrin is a toxic protein produced by the ornamental plantAbrus precatorius, and it is of concern as a biothreat agent. The small coextracting moleculeN-methyl-l-tryptophan (l-abrine) is specific to members of the genusAbrusand thus can be used as a marker for the presence or ingestion of abrin. Current methods for the detection of abrin orl-abrine in foods and other matrices require complex sample preparation and expensive instrumentation. To develop a fast and portable method for the detection ofl-abrine in beverages and foods, theEscherichia coliproteinsN-methyltryptophan oxidase (MTOX) and tryptophanase were expressed and purified. The two enzymes jointly degradedl-abrine to products that included ammonia and indole, and colorimetric assays for the detection of those analytes in beverage and food samples were evaluated. An indole assay using a modified version of Ehrlich's/Kovac's reagent was more sensitive and less subject to negative interferences from components in the samples than the Berthelot ammonia assay. The two enzymes were added into food and beverage samples spiked withl-abrine, and indole was detected as a degradation product, with the visual lower detection limit being 2.5 to 10.0 μM (∼0.6 to 2.2 ppm)l-abrine in the samples tested. Results could be obtained in as little as 15 min. Sample preparation was limited to pH adjustment of some samples. Visual detection was found to be about as sensitive as detection with a spectrophotometer, especially in milk-based matrices.

scholarly journals Application of Rapid, Electrochemical Flash Titration™ to Total Acidity and Alkalinity Determinations in Buffers, Foods, and Beverages

2004 ◽  
Vol 87 (5) ◽  
pp. 1208-1217 ◽  
Author(s):  
Xiaowen Wen ◽  
Janet Herdan ◽  
Steven West ◽  
Denise Kinkade ◽  
Nadejda Vilissova ◽  
...  
Keyword(s):  
Ph Sensor ◽  
Food Products ◽  
Metal Electrode ◽  
Total Alkalinity ◽  
Total Acidity ◽  
Titration Method ◽  
Time Saving ◽  
End Point ◽  
Food And Beverage ◽  
Beverage Analysis

Abstract A new technique (Flash Titration™) for the determination of total acidity and total alkalinity was applied to food and beverage analysis. Requiring no liquid titrants, Flash Titration is a technique made possible through microfabrication of electrochemical components on a silicon chip. Acidic or basic titrant was generated electrochemically at a noble-metal electrode that intimately surrounded an ion-selective, field-effect transistor pH sensor. As acid or base was generated through electrolysis, sample alkalinity or acidity, respectively, was neutralized in the immediate vicinity of the electrode. Through diffusion, a zone comprising a gradient of partially to totally neutralized sample expanded from the generating electrode into the volume element sensed by the nearby pH sensor. An analysis of the pH signal versus time revealed an end point inflection at an elapsed time related to the total alkalinity or acidity of the sample. End point times were typically a few seconds. In this paper, Flash Titration was applied to the analysis of a variety of samples, including juices, soft drinks, wines, and food products. The differences between the results obtained by the Flash Titration method and a commonly used conventional volumetric method were less than 2% in most food products tested. Analysis costs were reduced both through time saving and reduction or elimination of hazardous liquid titrant disposal, by the use of the Flash Titration method.

scholarly journals Cover, Content, and Editorial Note from Borneo J Med Lab Tech Vol. 3 No. 2 April 2021

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Chief Editor Of Borneo J Med Lab Tech
Keyword(s):  
Technology Development ◽  
Clinical Chemistry ◽  
Scientific Journal ◽  
Medical Laboratory ◽  
Editorial Note ◽  
Editorial Boards ◽  
Food And Beverage ◽  
Laboratory Technology ◽  
Beverage Analysis

Assalamu’alaikum Wr. Wb.Alhamdulillahirabbil ‘alamin. After a long wait for almost 12 years from the first stand, finally the scientific journal of the Department of Analyst Health (Medical Laboratory Technology) Universitas Muhammadiyah Palangkaraya can be published. BJMLT published every 6 months (2 issues/year) every October and April. The aim of this journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field but not limited to Blood transfusion, Clinical Chemistry, Hematology, Histopathology, Immunology, Microbiology, Parasitology, Toxicology, Food and Beverage Analysis, Molecular Biology, Medical Biology, and related fields that are of interest to medical laboratory technologies, such as epidemiology, health promotion, professional ethics, and laboratory management, It was first published online in October 2018 using Open Journal System (OJS) 3.1 platform with e-ISSN 2622-6111 by URL http://journal.umpalangkaraya.ac.id/index.php/bjmlt.This edition contains 7 articles consisting of Hematology, Microbiology, Clinical Chemistry, and epidemiology topics. Editorial boards are fully aware that there is still room for improvement in this edition, hence with all humility willing to accept constructive suggestions and feedback for improvements to the publication for the next editions.The publication of BJMLT certainly participates in disseminating the results of research and review of science and technology development conducted by lecturers and researchers from UM Palangkaraya and other universities.The editorial board would like to thank the University, all editors and reviewers, and contributors of the scientific articles who have provided the repertoire in this issue. We hope that all parties, especially the contributors of the articles, could re-participate for the success of the publication in the next edition in October 2021. Wassalamu’alaikum Wr. Wb.

scholarly journals Cover, Content, and Editorial Note from Borneo J Med Lab Tech Vol. 2 No. 2 April 2020

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Chief Editor Of Borneo J Med Lab Tech
Keyword(s):  
Technology Development ◽  
Clinical Chemistry ◽  
Scientific Journal ◽  
Medical Laboratory ◽  
Editorial Note ◽  
Editorial Boards ◽  
Food And Beverage ◽  
Laboratory Technology ◽  
Beverage Analysis

Assalamu�alaikum Wr. Wb.Alhamdulillahirabbil �alamin. After a long wait for almost 12 years from the first stand, finally the scientific journal of the Department of Analyst Health (Medical Laboratory Technology) Universitas Muhammadiyah Palangkaraya can be published. BJMLT published every 6 months (2 issues/year) every October and April. The aim of this journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field but not limited to Blood transfusion, Clinical Chemistry, Hematology, Histopathology, Immunology, Microbiology, Parasitology, Toxicology, Food and Beverage Analysis, Molecular Biology, Medical Biology, and related fields that are of interest to medical laboratory technologies, such as epidemiology, health promotion, professional ethics, and laboratory management, It was first published online in October 2018 usingOpen Journal System (OJS) 3.1 platform with e-ISSN 2622-6111 by url http://journal.umpalangkaraya.ac.id/index.php/bjmlt.This edition contains 6 articles consisting of Hematology, Microbiology, Clinical Chemistry and epidemiology topics. Editorial boards are fully aware that there are still room for improvement in this edition, hence with all humility willing to accept constructive suggestions and feedback for improvements to the publication for the next editions.The publication of BJMLTcertainly participate in disseminating the results of research and review of science and technology development conducted by lecturers and researchers from UM Palangkaraya and other universities.The editorial board would like to thank the University, all editors and reviewers, and contributors of the scientific articles who have provided the repetoire in this issue. We hope that all parties, especially the contributors of the articles, could re-participate for the success of the publication in the next edition on october 2020.Wassalamu�alaikum Wr. Wb.Palangka Raya, April 2020Editor-in-Chief

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