scholarly journals Umbilical Cord Blood Hematopoietic Stem and Progenitor Cell Expansion for Therapeutic Use

10.5772/64851 ◽  
2017 ◽  
Author(s):  
Suzanne M. Watt ◽  
Peng Hua
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cell Expansion ◽  
Progenitor Cell ◽  
Therapeutic Use ◽  
Hematopoietic Stem

A modified CD34+ hematopoietic stem and progenitor cell isolation strategy from cryopreserved human umbilical cord blood

Transfusion ◽  
2019 ◽  
Vol 59 (12) ◽  
pp. 3560-3569
Author(s):  
Marcia F. Mata ◽  
Diana Hernandez ◽  
Evangelia Rologi ◽  
Davide Grandolfo ◽  
Enas Hassan ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Progenitor Cell ◽  
Cell Isolation ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Hematopoietic Stem

A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on immunoaffinity aqueous two-phase partitioning

2011 ◽  
Vol 33 (12) ◽  
pp. 2373-2377 ◽  
Author(s):  
António F. Sousa ◽  
Pedro Z. Andrade ◽  
Roksana M. Pirzgalska ◽  
Teresa M. Galhoz ◽  
Ana M. Azevedo ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Progenitor Cell ◽  
Cell Isolation ◽  
Two Phase ◽  
Hematopoietic Stem ◽  
Phase Partitioning ◽  
Novel Method

Fucosylated umbilical cord blood hematopoietic stem cell expansion on selectin‐coated scaffolds

2019 ◽  
Vol 234 (12) ◽  
pp. 22593-22603 ◽  
Author(s):  
Maryam Islami ◽  
Zahra Payandeh ◽  
Elaheh Dalir Abdolahinia ◽  
Ehsan Saburi ◽  
Fatemeh Soleimanifar ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Hematopoietic Stem Cell ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cell Expansion ◽  
Hematopoietic Stem ◽  
Stem Cell Expansion ◽  
Hematopoietic Stem Cell Expansion

Protocols for Hematopoietic Stem Cell Expansion from Umbilical Cord Blood

2009 ◽  
Vol 18 (10-11) ◽  
pp. 1059-1068 ◽  
Author(s):  
Sonja Koestenbauer ◽  
Andreas Zisch ◽  
Gottfried Dohr ◽  
Nicolas H. Zech
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Hematopoietic Stem Cell ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cell Expansion ◽  
Hematopoietic Stem ◽  
Stem Cell Expansion ◽  
Hematopoietic Stem Cell Expansion

scholarly journals Positive Correlation between Very Small Embryonic Stem Cell, Hematopoietic Stem Cell, and Endothelial Progenitor Cell in Umbilical Cord Blood Unit

2018 ◽  
Vol 10 (3) ◽  
pp. 231-5
Author(s):  
Angliana Chouw ◽  
Bayu Winata Putera ◽  
Cynthia Retna Sartika ◽  
Ajeng Diantini
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Cell Number ◽  
Total Cell ◽  
Endothelial Progenitor ◽  
Hematopoietic Stem

BACKGROUND: Umbilical Cord Blood (UCB) has been widely use as regenerative medicine due to the content of undifferentiated cell which have capability to do self-renewal and differentiation into various type of cell called stem cells. Recent studies show that UCB contains not only hematopoietic stem cell (HSC) but also others stem cell and progenitor cell such as endothelial progenitor cell (EPC) and very small embryonic-like stem cell (VSEL). It is beliefs that HSC and EPC shared the same progenitor. In this study, correlation between the cell number of HSC, EPC and VSEL is analyzed in umbilical cord blood as the source of stem cell for clinical application.METHODS: The cell number of HSC, EPC and VSEL is counted from cryopreserved UCB collected from 22 women delivered via cesarean section which already stored for more than 2 years in this study. Sample were incubated with antibodies such as cluster of differentiation (CD)34-phycoerythrin (PE)/CD45-fluorescein isothiocyanate (FITC), CD133/1 (anti-CD (AC)133)-antigen-presenting cell (APC) and, CD184 (C-X-C chemokine receptor (CXCR)4)-PE.Vio770 to detect the present of HSC, EPC and VSEL in UCB. Sample were analyze using flowcytometer BD FACS Canto II.RESULTS: The cell population of HSC and late-EPC is 0.009% and 0.01% of total cell in UCB. VSEL only represented 0.001% from total cell in UCB, showing the lowest number of cell population in UCB. The correlation between the cell number of HSC and EPC is r=0,483*, p=0.023) and between HSC and VSEL is r=0.510*, p=0.015.CONCLUSION: In this study, both EPC and VSEL have a significant positive correlation with HSC.KEYWORDS: stem cell, umbilical cord blood, endothelial progenitor, flowcytometry

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