scholarly journals Biosensors for Rapid Detection of Avian Influenza

10.5772/64353 ◽  
2016 ◽  
Author(s):  
Ronghui Wang ◽  
Yanbin Li
Keyword(s):  
Avian Influenza ◽  
Rapid Detection

Development of an antigen-ELISA and a colloidal gold–based immunochromatographic strip based on monoclonal antibodies for detection of avian influenza A(H5) viruses

2021 ◽  
pp. 104063872110275
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Linfang Cheng ◽  
Hangping Yao ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Disease Virus ◽  
Colloidal Gold ◽  
Rapid Detection ◽  
Influenza A ◽  
Field Investigation ◽  
Cross Reactivity ◽  
Detection Methods ◽  
Immunochromatographic Strip ◽  
Antigen Elisa

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold–based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e.g., other avian IAVs, infectious bursal disease virus, Newcastle disease virus, infectious bronchitis virus, avian paramyxovirus). The ag-ELISA was sensitive down to 0.5 hemagglutinin (HA) units/100 µL of avian A(H5) viruses and 7.5 ng/mL of purified H5 HA proteins. The immunochromatographic strip was sensitive down to 1 HA unit/100 µL of avian A(H5) viruses. Both detection methods exhibited good reproducibility with CVs < 10%. For 200 random poultry samples, the sensitivity and specificity of the ag-ELISA were 92.6% and 98.8%, respectively, and for test strips were 88.9% and 98.3%, respectively. Both detection methods displayed high specificity, sensitivity, and stability, making them suitable for rapid detection and field investigation of avian A(H5) viruses.

scholarly journals Development of an Immunochromatographic Strip for Rapid Detection of H9 Subtype Avian Influenza Viruses

2008 ◽  
Vol 15 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Fuhu Peng ◽  
Zheng Wang ◽  
Shuhui Zhang ◽  
Renwei Wu ◽  
Sishun Hu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Rapid Detection ◽  
Clinical Symptoms ◽  
High Specificity ◽  
Cloacal Swab ◽  
Influenza Viruses ◽  
Control Line ◽  
Avian Influenza Viruses ◽  
Immunochromatographic Strip

ABSTRACT An immunochromatographic strip was developed for the detection of the H9 subtype of avian influenza viruses (H9AIVs) in poultry, using two monoclonal antibodies (MAb), 4C4 for H9AIV hemagglutinin (HA) and 4D4 for nucleoprotein. The 4C4 MAb was labeled with colloidal gold as the detection reagent, and the 4D4 MAb was blotted on the test line while a goat anti-mouse antibody was used on the control line of the nitrocellulose membrane. In comparison with the HA and HA inhibition (HI) tests, the strip was specific for the detection of H9AIV, with a sensitivity at 0.25 HA units within 10 min. Storage of the strips at room temperature for 6 months or at 4°C for 12 months did not change their sensitivity and specificity. Evaluation of the strip with experimental tracheal and cloacal swab samples collected from H9N2-infected chickens revealed that the strip detected the H9N2 viruses on day 3 postinoculation, earlier than the appearance of clinical symptoms. Application of the strip for the analysis of 157 tracheal or cloacal samples from potentially infected chickens on five poultry farms showed that four farms had chickens that were infected with H9AIV. Further characterization of 10 positive and 30 negative randomly selected samples showed that no single sample was false positive or negative, as determined by the standard virus isolation and HI assays. Therefore, the immunochromatographic strip for the detection of H9AIVs has high specificity, sensitivity, and stability. This finding, together with the advantages of rapid detection and easy operation and without the requirement for special skills and equipment, makes the strip suitable for onsite detection and the differentiation of H9AIVs from other viruses in poultry.

Development of a TaqMan MGB RT-PCR for the rapid detection of H3 subtype avian influenza virus circulating in China

2015 ◽  
Vol 217 ◽  
pp. 64-69 ◽  
Author(s):  
Qiaoyang Teng ◽  
Weixia Shen ◽  
Dawei Yan ◽  
Liping Yan ◽  
Xuesong Li ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Rapid Detection ◽  
Rt Pcr ◽  
H3 Subtype

Rapid Detection of Avian Influenza Virus Using Immunomagnetic Separation and Impedance Measurement

2012 ◽  
Vol 239-240 ◽  
pp. 367-371
Author(s):  
Xiao Fei Yan ◽  
Mao Hua Wang ◽  
Xin Hua Wen ◽  
Dong An
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Rapid Detection ◽  
Impedance Measurement ◽  
Immunomagnetic Separation ◽  
Impedance Change ◽  
Equivalent Circuit Analysis ◽  
Medium Resistance ◽  
Impedance Magnitude

An interdigitated array microelectrodes (IDAMs) based impedance biosensor in combination with immunomagnetic separation was developed for rapid detection of avian influenza virus (AIV) subtype H5. Streptavidin-coated magnetic nanobeads were immobilized onto the biotin-labeled anti-H5 monoclonal antibodies to capture AIV H5 (e.g., H5N1) from sample solutions by the specific immunoreaction and form antibodies coated nanobeads-AIV complexes. Then these complexes were separated and concentrated by a magnetic field and the impedance magnitude was measured by IDAMs in a frequency range from 20 Hz to 1 MHz. The sensitivity and specificity of this biosensor were investigated. The biosensor could detect as few as 2-1 HA unit/50 μl of inactivated AIV H5N1. A linear relationship between the change of impedance magnitude and the logarithmic value of AIV H5N1 concentration was found in the range of 2-1 to 24 HA unit/50 μl. Non-target viruses, such as AIV subtype H1 and Newcastle disease virus, could not induce detectable signals. Equivalent circuit analysis showed that the medium resistance was responsible for the impedance change caused by the presence of AIV H5N1. The whole detection process from sampling to impedance measurement was able to be completed within 1.5 h.

Sign in / Sign up

Export Citation Format

Share Document