scholarly journals New and Improved Tissue Engineering Techniques: Production of Exogenous Material-Free Stroma by the Self-Assembly Technique

10.5772/62588 ◽  
2016 ◽  
Author(s):  
Ingrid Saba ◽  
Weronika Jakubowska ◽  
Stéphane Chabaud ◽  
Stéphane Bolduc
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
The Self ◽  
Assembly Technique

scholarly journals Review: The Self-Assembly Approach for Organ Reconstruction by Tissue Engineering

2000 ◽  
Vol 1 (5) ◽  
pp. 75-86 ◽  
Author(s):  
F.A. Auger ◽  
M. Rémy-Zolghadri ◽  
G. Grenier ◽  
L. Germain
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
The Self

Surface Modification of Polydimethylsiloxane Using Low Pressure Chemical Vapour Deposition of Poly-Chloro-p-Xylene

2012 ◽  
Vol 20 ◽  
pp. 129-142 ◽  
Author(s):  
Paul Emile Poleni ◽  
Nazare Pereira-Rodrigues ◽  
Denis Guimard ◽  
Yasuhiko Arakawa ◽  
Yasuyuki Sakai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Vapour Deposition ◽  
Type I Collagen ◽  
Chemical Vapour ◽  
The Self ◽  
Type I ◽  
Liver Tissue Engineering ◽  
Pressure Chemical ◽  
Huvec Cells

The capability to understand and modulate accurately the self-assembly of the extracellular matrix (ECM) components still one of the major fundamental objectives in the field of liver tissue engineering. In the present study, we put in evidence the suitability of poly-chloro-p-xylene (Parylene-C, ParC) for modulating the self-assembly of ECM (type-I collagen) microenvironment and cellular topography of human hepatocarcinoma (HepG2) and Human umbilical vascular endothelial (HUVEC) cells while coated on a polydimethylsiloxane (PDMS) substratum. Our findings demonstrated that the wettability of PDMS and ParC/PDMS were identical, while ParC/PDMS was significantly rougher than PDMS before and after collagen coating. However, the roughness and the wettability of ParC/PDMS were comparable to those of polystyrene (PS), a substratum commonly used for in vitro biological-related investigations. Type-I collagen adsorbed on ParC/PDMS and PS exhibited a dense network of microstructures around ~1 nm high and ~30-50 nm wide, whereas collagen adsorbed on PDMS had a low surface density of elongated fibrils that were ~2 nm thick and ~200 nm wide. This disparity in ECM microarchitecture leaded to distinct culture topographies of HepG2 cells (3D and 2D for PDMS and ParC/PDMS, respectively) and viability of HUVEC (2D viable HUVEC cells and non attached dead cells on ParC/PDMS and PDMS, respectively). To conclude, the observed changes in cell morphology and viability between ParC/PDMS and PDMS alone were directly related to the nature of the material which may impact the supramolecular organization of adsorbed ECM. We strongly believe that Low Pressure Chemical Vapour deposition (LPCVD) of ParC will offer promising insights into how microscale ECM modifications directly impact cell morphology and activity, leading to the development of advanced micro/nanosized tissue-engineered ParC/PDMS patterns with applications for liver tissue engineering.

scholarly journals Human Organ-Specific 3D Cancer Models Produced by the Stromal Self-Assembly Method of Tissue Engineering for the Study of Solid Tumors

2020 ◽  
Vol 2020 ◽  
pp. 1-23 ◽  
Author(s):  
Vincent Roy ◽  
Brice Magne ◽  
Maude Vaillancourt-Audet ◽  
Mathieu Blais ◽  
Stéphane Chabaud ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tumor Microenvironment ◽  
Self Assembly ◽  
The Self ◽  
Human Organ ◽  
Assembly Method ◽  
Cancer Models ◽  
Organ Specific

Cancer research has considerably progressed with the improvement of in vitro study models, helping to understand the key role of the tumor microenvironment in cancer development and progression. Over the last few years, complex 3D human cell culture systems have gained much popularity over in vivo models, as they accurately mimic the tumor microenvironment and allow high-throughput drug screening. Of particular interest, in vitrohuman 3D tissue constructs, produced by the self-assembly method of tissue engineering, have been successfully used to model the tumor microenvironment and now represent a very promising approach to further develop diverse cancer models. In this review, we describe the importance of the tumor microenvironment and present the existing in vitro cancer models generated through the self-assembly method of tissue engineering. Lastly, we highlight the relevance of this approach to mimic various and complex tumors, including basal cell carcinoma, cutaneous neurofibroma, skin melanoma, bladder cancer, and uveal melanoma.

Photocatalytic Activity of Pd Doped Tin Dioxide Inverse Opal Films

2012 ◽  
Vol 534 ◽  
pp. 135-140 ◽  
Author(s):  
Yang Liu ◽  
Bo Li ◽  
You Wei Yao ◽  
Jing Jing Gao ◽  
Zhen Dong Liu ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photocatalytic Activity ◽  
Self Assembly ◽  
Tin Dioxide ◽  
Uv Light ◽  
Sol Gel ◽  
The Self ◽  
Inverse Opal ◽  
Polystyrene Microsphere ◽  
Assembly Technique

Inverse opal films (IOFs) of SnO2 doped with Pd were prepared by the self-assembly technique using polystyrene microsphere (PS sphere) as template in combination with a sol-gel method. The photocatalysis properties of SnO2 IOFs were estimated through measuring the rate of the degradation of methylene blue (MB). The result shows that SnO2 IOFs have good photocatalytic activity, the solution of MB was degradated over 60% in 4 hours when it was dipped in SnO2 IOFs and exposed in the UV light. The addition of Pd in SnO2 IOFs improved the photocatalytic activity of the films and the degradation of MB can exceed 80% with the same condition. This sort of SnO2 IOFs doped with Pd indicated a potential application in photocatalysis field.

Preparation of parent polyaniline thin films using the self-assembly technique

1999 ◽  
Vol 101 (1-3) ◽  
pp. 726
Author(s):  
R.S. Pontes ◽  
A. Dhanabalan ◽  
C.S. Camilo ◽  
M. Ferreira ◽  
O.N. Oliveira
Keyword(s):  
Thin Films ◽  
Self Assembly ◽  
The Self ◽  
Assembly Technique

scholarly journals Engineering Tissues without the Use of a Synthetic Scaffold: A Twenty-Year History of the Self-Assembly Method

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Ingrid Saba ◽  
Weronika Jakubowska ◽  
Stéphane Bolduc ◽  
Stéphane Chabaud
Keyword(s):  
Extracellular Matrix ◽  
Self Assembly ◽  
Large Scale ◽  
Dermal Fibroblasts ◽  
The Self ◽  
Production Costs ◽  
Scale Production ◽  
Assembly Method ◽  
Assembly Technique

Twenty years ago, Dr. François A. Auger, the founder of the Laboratory of Experimental Organogenesis (LOEX), introduced the self-assembly technique. This innovative technique relies on the ability of dermal fibroblasts to produce and assemble their own extracellular matrix, differing from all other tissue-engineering techniques that use preformed synthetic scaffolds. Nevertheless, the use of the self-assembly technique was limited for a long time due to its main drawbacks: time and cost. Recent scientific breakthroughs have addressed these limitations. New protocol modifications that aim at increasing the rate of extracellular matrix formation have been proposed to reduce the production costs and laboratory handling time of engineered tissues. Moreover, the introduction of vascularization strategies in vitro permits the formation of capillary-like networks within reconstructed tissues. These optimization strategies enable the large-scale production of inexpensive native-like substitutes using the self-assembly technique. These substitutes can be used to reconstruct three-dimensional models free of exogenous materials for clinical and fundamental applications.

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