scholarly journals Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication

10.5772/62580 ◽  
2016 ◽  
Vol 5 ◽  
pp. 3 ◽  
Author(s):  
Anne Louise Schacht Revenfeld ◽  
Evo Kristina Lindersson Søndergaard ◽  
Allan Stensballe ◽  
Rikke Bæk ◽  
Malene Møller Jørgensen ◽  
...  
Keyword(s):  
Extracellular Vesicle ◽  
Cell Culturing ◽  
A Cell

scholarly journals Quantitative characterization of extracellular vesicle uptake and content delivery within mammalian cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Emeline Bonsergent ◽  
Eleonora Grisard ◽  
Julian Buchrieser ◽  
Olivier Schwartz ◽  
Clotilde Théry ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Fluorescent Protein ◽  
Extracellular Vesicle ◽  
Content Delivery ◽  
Bafilomycin A1 ◽  
Quantitative Characterization ◽  
Endosomal Acidification ◽  
A Cell ◽  
Dose Responses

AbstractExtracellular vesicles (EVs), including exosomes, are thought to mediate intercellular communication through the transfer of cargoes from donor to acceptor cells. Occurrence of EV-content delivery within acceptor cells has not been unambiguously demonstrated, let alone quantified, and remains debated. Here, we developed a cell-based assay in which EVs containing luciferase- or fluorescent-protein tagged cytosolic cargoes are loaded on unlabeled acceptor cells. Results from dose-responses, kinetics, and temperature-block experiments suggest that EV uptake is a low yield process (~1% spontaneous rate at 1 h). Further characterization of this limited EV uptake, through fractionation of membranes and cytosol, revealed cytosolic release (~30% of the uptaken EVs) in acceptor cells. This release is inhibited by bafilomycin A1 and overexpression of IFITM proteins, which prevent virus entry and fusion. Our results show that EV content release requires endosomal acidification and suggest the involvement of membrane fusion.

scholarly journals Characterization of a cell mutant specifically defective in the synthesis of anticoagulantly active heparan sulfate.

1994 ◽  
Vol 269 (40) ◽  
pp. 24953-24958 ◽  
Author(s):  
S. Colliec-Jouault ◽  
N.W. Shworak ◽  
J. Liu ◽  
A.I. de Agostini ◽  
R.D. Rosenberg
Keyword(s):  
Heparan Sulfate ◽  
Cell Mutant ◽  
A Cell

Characterization of a Cell Line Derived From a Human Giant Cell Tumor That Stimulates Osteoclastic Bone Resorption

1993 ◽  
Vol &NA; (296) ◽  
pp. 229???241 ◽  
Author(s):  
RICHARD O. C. OREFFO ◽  
G. JUNE MARSHALL ◽  
MARY KIRCHEN ◽  
CARLOS GARCIA ◽  
WOLF E. GALLWITZ ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Giant Cell Tumor ◽  
Cell Line ◽  
Giant Cell ◽  
Cell Tumor ◽  
Osteoclastic Bone Resorption ◽  
A Cell

Isolation and characterization of a cell-associated protein of Bacillus pumilus PH-01

2001 ◽  
Vol 56 (3-4) ◽  
pp. 402-405 ◽  
Author(s):  
H.-B. Hong ◽  
Y.-S. Chang ◽  
S.-D. Choi ◽  
I.-H. Nam ◽  
Y.-E. Lee
Keyword(s):  
Bacillus Pumilus ◽  
Isolation And Characterization ◽  
A Cell
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