scholarly journals Role of Organic Sources of Nutrients in Rice (Oryza sativa) Based on High Value Cropping Sequence

10.5772/61841 ◽  
2016 ◽  
Author(s):  
Sanjay Kumar Yadav ◽  
Subhash Babu ◽  
Gulab Singh Yadav ◽  
Raghavendra Singh ◽  
Manoj Kumar Yadav
Keyword(s):  
Oryza Sativa ◽  
Cropping Sequence

scholarly journals Role of Microbiome: Insights into the Kin Recognition Process in Oryza Sativa by Proteomic and Metabolomic Studies

2017 ◽  
pp. 133-153
Author(s):  
Anjana Devi Tangutur ◽  
Kommalapati Vamsi Krishna ◽  
Amrita Dutta Chowdhury ◽  
Neelamraju Sarla
Keyword(s):  
Oryza Sativa ◽  
Kin Recognition ◽  
Recognition Process

scholarly journals OsmiR535, a Potential Genetic Editing Target for Drought and Salinity Stress Tolerance in Oryza sativa

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1337
Author(s):  
Erkui Yue ◽  
Huan Cao ◽  
Bohan Liu
Keyword(s):  
Oryza Sativa ◽  
Stress Response ◽  
Molecular Breeding ◽  
Mirna Family ◽  
Plant Responses ◽  
Practical Application ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Post Stress

OsmiR535 belongs to the miR156/miR529/miR535 superfamily, a highly conserved miRNA family in plants. OsmiR535 is involved in regulating the cold-stress response, modulating plant development, and determining panicle architecture and grain length. However, the role that OsmiR535 plays in plant responses to drought and salinity are elusive. In the current study, molecular and genetic engineering techniques were used to elucidate the possible role of OsmiR535 in response to NaCl, PEG(Poly ethylene glycol), ABA(Abscisic acid), and dehydration stresses. Our results showed that OsmiR535 is induced under stressed conditions as compared to control. With transgenic and CRISPR/Cas9 knockout system techniques, our results verified that either inhibition or knockout of OsmiR535 in rice could enhance the tolerance of plants to NaCl, ABA, dehydration and PEG stresses. In addition, the overexpression of OsmiR535 significantly reduced the survival rate of rice seedlings during PEG and dehydration post-stress recovery. Our results demonstrated that OsmiR535 negatively regulates the stress response in rice. Moreover, our practical application of CRISPR/Cas9 mediated genome editing created a homozygous 5 bp deletion in the coding sequence of OsmiR535, demonstrating that OsmiR535 could be a useful genetic editing target for drought and salinity tolerance and a new marker for molecular breeding of Oryza sativa.

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