scholarly journals Cultural, Physiological, and Biochemical Identification of Actinobacteria

Author(s):  
Qinyuan Li ◽  
Xiu Chen ◽  
Yi Jiang ◽  
Chenglin Jiang
2021 ◽  
Vol 251 ◽  
pp. 02037
Author(s):  
Junjiao Zhang ◽  
Wenjing Li

In recent years, biological desulfurization technology has attracted more and more attention from domestic and foreign researchers due to its mild reaction conditions, high efficiency, and environmental protection. In this study, bacteria were collected from oil depots, sewage treatment plants and sewage, and using a screening medium with DBT as the only sulfur source. Three strains that can degrade DBT were isolated, numbered 1, 2, and 3. Through the identification of morphological characteristics, physiological and biochemical identification and molecular identification, it is preliminarily determined that the bacteria No. 1 belongs to the genus Kinetococcus, the bacteria No. 2 is Micrococcus mutans, the bacteria No. 3 is Micrococcus luteus, and the desulfurization capacity of the three bacteria is No. 3. Bacteria > Bacteria No.1 > Bacteria No.2.


2013 ◽  
Vol 316-317 ◽  
pp. 594-598 ◽  
Author(s):  
Wen Jing Hao ◽  
Jun Mu ◽  
Yuan Li

In this experiment, 25 oil degrading bacteria strains were isolated from the beach near Dalian Bay. The top 4 high efficient oil-degrading bacteria(SQ、SY 、SW、SC) were selected to be studied. Using Physiological and biochemical identification, electron microscopy, and 16S rDNA sequence analysis, strain SQ was identified as Advenella kashmirensis,SY was identified as Achromobacter xylosoxidans, SW was identified as Acinetobacter venetianus,SC was identified as Alcaligenes xylosoxidans subsp.xylosoxidans. For the first time this experiment provided reference to application and degradation characteristics of Advenella kashmirensis in crude oil treatment. These four marine oil degrading bacteria were selected to form bacterial consortium. The most efficient mixed strains were constructed by using these four strains with proportional combination. The oil removal ratio of the optimal mixed strains increased to 70.33%, nearly 25% higher than the highest degradation rate of single bacterium, showing significant synergism in the bacterial consortium. Advenella kashmirensis enhanced oil degradation obviously, playing a crucial role in the bacterial consortium.


2012 ◽  
Vol 518-523 ◽  
pp. 2030-2033 ◽  
Author(s):  
Jia Niu ◽  
Ji Hua Wang ◽  
Di Cui ◽  
Xiang Liu ◽  
Hui Guang

A Gram positive bacterium strain 12-3 for degrading DDT effectively was isolated from the DDT contaminated site of the shipyard in Guangzhou by enrichment culture, which could utilize DDT as the sole carbon source for growth. This strain was identified as Pseudomonas species. Based on the phenotype, physiological and biochemical identification, and fatty acids identification. Testing theirs DDT degradation rate with HPLC, the results showed that in a shaky flask containing 20 mg/L DDT, this strain could degrade DDT with degradation efficiency of 51.6% in 8 days at 30°C, pH 8.0.


2013 ◽  
Vol 65 (1) ◽  
pp. 133-150 ◽  
Author(s):  
Mady A. Ismail ◽  
Sobhy I. I. Abdel-Hafez ◽  
Nemmat A. Hussein ◽  
Nevein A. Abdel-Hameed

2017 ◽  
Vol 52 (6) ◽  
pp. 1129-1138 ◽  
Author(s):  
K.A. Petrov ◽  
◽  
A.A. Perk ◽  
V.A. Chepalov ◽  
V.E. Sofronova ◽  
...  

2007 ◽  
Vol 43 (1) ◽  
pp. 95-105
Author(s):  
O. G. Zinkovskiy ◽  
V. D. Solomatina ◽  
A. S. Potrokhov ◽  
N. A. Mogilevich

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