scholarly journals Transmission Electron Microscopy of Biological Samples

10.5772/60680 ◽  
2015 ◽  
Author(s):  
Łukasz Mielańczyk ◽  
Natalia Matysiak ◽  
Olesya Klymenko ◽  
Romuald Wojnicz
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Biological Samples ◽  
Transmission Electron

scholarly journals Direct Observation of Wet Biological Samples by Graphene Liquid Cell Transmission Electron Microscopy

Nano Letters ◽  
2015 ◽  
Vol 15 (7) ◽  
pp. 4737-4744 ◽  
Author(s):  
Jungwon Park ◽  
Hyesung Park ◽  
Peter Ercius ◽  
Adrian F. Pegoraro ◽  
Chen Xu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Direct Observation ◽  
Biological Samples ◽  
Transmission Electron ◽  
Cell Transmission ◽  
Liquid Cell

Soft X-ray contact microscopy and transmission electron microscopy: Comparative study of biological samples

2003 ◽  
Vol 104 ◽  
pp. 345-348 ◽  
Author(s):  
T. Limongi ◽  
L. Palladino ◽  
E. Bernieri ◽  
G. Tomassetti ◽  
L. Reale ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Comparative Study ◽  
Biological Samples ◽  
X Ray ◽  
Transmission Electron

scholarly journals Energy-filtered transmission electron microscopy of biological samples on highly transparent carbon nanomembranes

2011 ◽  
Vol 111 (5) ◽  
pp. 342-349 ◽  
Author(s):  
Daniel Rhinow ◽  
Matthias Büenfeld ◽  
Nils-Eike Weber ◽  
André Beyer ◽  
Armin Gölzhäuser ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Biological Samples ◽  
Transmission Electron

Preparation Methods for EM Studies of Polymers

2012 ◽  
Vol 20 (2) ◽  
pp. 46-49
Author(s):  
Helen Hassander
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Biological Samples ◽  
Organic Material ◽  
Organic Materials ◽  
Saturated Hydrocarbon ◽  
Preparation Methods ◽  
Latex Particles ◽  
Transmission Electron ◽  
Reactive Groups

Polymers are organic materials, and most of the preparation methods for transmission electron microscopy (TEM) are much the same as for biological specimens. Bulk samples are cut in the ultramicrotome, and latex particles are dropped onto a Formvar-coated grid. However, the staining possibilities of polymers are limited. Polymers mainly consist of saturated hydrocarbon, and unlike biomaterial there are few reactive groups for staining. This has two consequences for the microscopist: it is difficult to obtain contrast, and the sample is very beam-sensitive. Biological samples are also organic material and sensitive to the beam, but the staining process converts them into materials that are usually stable to the beam.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

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