scholarly journals Whole Tooth Regeneration Using a Bioengineered Tooth

10.5772/58908 ◽  
2014 ◽  
Author(s):  
Masamitsu Oshima ◽  
Takashi Tsuji
Keyword(s):  
Tooth Regeneration ◽  
Bioengineered Tooth

scholarly journals Functional Tooth Regeneration Using a Bioengineered Tooth Unit as a Mature Organ Replacement Regenerative Therapy

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e21531 ◽  
Author(s):  
Masamitsu Oshima ◽  
Mitsumasa Mizuno ◽  
Aya Imamura ◽  
Miho Ogawa ◽  
Masato Yasukawa ◽  
...  
Keyword(s):  
Regenerative Therapy ◽  
Tooth Regeneration ◽  
Functional Tooth ◽  
Organ Replacement Regenerative Therapy ◽  
Bioengineered Tooth ◽  
Organ Replacement

scholarly journals Anti–USAG-1 therapy for tooth regeneration through enhanced BMP signaling

2021 ◽  
Vol 7 (7) ◽  
pp. eabf1798
Author(s):  
A. Murashima-Suginami ◽  
H. Kiso ◽  
Y. Tokita ◽  
E. Mihara ◽  
Y. Nambu ◽  
...  
Keyword(s):  
Tooth Development ◽  
Bmp Signaling ◽  
Tooth Agenesis ◽  
Tooth Regeneration ◽  
Missing Teeth ◽  
Morphogenetic Protein ◽  
Genetic Abnormalities ◽  
Direct Binding ◽  
Tooth Germs ◽  
Lipoprotein Receptor Related Protein

Uterine sensitization–associated gene-1 (USAG-1) deficiency leads to enhanced bone morphogenetic protein (BMP) signaling, leading to supernumerary teeth formation. Furthermore, antibodies interfering with binding of USAG-1 to BMP, but not lipoprotein receptor–related protein 5/6 (LRP5/6), accelerate tooth development. Since USAG-1 inhibits Wnt and BMP signals, the essential factors for tooth development, via direct binding to BMP and Wnt coreceptor LRP5/6, we hypothesized that USAG-1 plays key regulatory roles in suppressing tooth development. However, the involvement of USAG-1 in various types of congenital tooth agenesis remains unknown. Here, we show that blocking USAG-1 function through USAG-1 knockout or anti–USAG-1 antibody administration relieves congenital tooth agenesis caused by various genetic abnormalities in mice. Our results demonstrate that USAG-1 controls the number of teeth by inhibiting development of potential tooth germs in wild-type or mutant mice missing teeth. Anti–USAG-1 antibody administration is, therefore, a promising approach for tooth regeneration therapy.

Nanotechnology For Tooth Regeneration

2014 ◽  
Vol 5 (1) ◽  
pp. 32-37
Author(s):  
Waqar Ahmed ◽  
St John Crean
Keyword(s):  
Tooth Regeneration

scholarly journals Therapeutic potential of HERS spheroids in tooth regeneration

Theranostics ◽  
2020 ◽  
Vol 10 (16) ◽  
pp. 7409-7421
Author(s):  
Yufeng Duan ◽  
Xuebing Li ◽  
Sicheng Zhang ◽  
Shikai Wang ◽  
Tao Wang ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Tooth Regeneration

Bioengineered Teeth from Cultured Rat Tooth Bud Cells

2004 ◽  
Vol 83 (7) ◽  
pp. 523-528 ◽  
Author(s):  
M.T. Duailibi ◽  
S.E. Duailibi ◽  
C.S. Young ◽  
J.D. Bartlett ◽  
J.P. Vacanti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Engineering Applications ◽  
Adult Rat ◽  
Dental Tissues ◽  
Biodegradable Scaffolds ◽  
Bioengineered Tooth ◽  
Tooth Tissue

The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.

scholarly journals The alternative regenerative strategy of bearded dragon unveils the key processes underlying vertebrate tooth renewal

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Lotta Salomies ◽  
Julia Eymann ◽  
Imran Khan ◽  
Nicolas Di-Poï
Keyword(s):  
Deep Understanding ◽  
Functional Specialization ◽  
Tooth Regeneration ◽  
Vertebrate Species ◽  
Directional Growth ◽  
Dental Lamina ◽  
Bearded Dragon ◽  
Conventional Models ◽  
Slow Cycling ◽  
Gene Expression Levels

Deep understanding of tooth regeneration is hampered by the lack of lifelong replacing oral dentition in most conventional models. Here, we show that the bearded dragon, one of the rare vertebrate species with both polyphyodont and monophyodont teeth, constitutes a key model for filling this gap, allowing direct comparison of extreme dentition types. Our developmental and high-throughput transcriptomic data of microdissected dental cells unveils the critical importance of successional dental lamina patterning, in addition to maintenance, for vertebrate tooth renewal. This patterning process happens at various levels, including directional growth but also gene expression levels, dynamics, and regionalization, and involves a large number of yet uncharacterized dental genes. Furthermore, the alternative renewal mechanism of bearded dragon dentition, with dual location of slow-cycling cells, demonstrates the importance of cell migration and functional specialization of putative epithelial stem/progenitor niches in tissue regeneration, while expanding the diversity of dental replacement strategies in vertebrates.

scholarly journals Antagonistic interaction between Ezh2 and Arid1a coordinates root patterning and development via Cdkn2a in mouse molars

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Junjun Jing ◽  
Jifan Feng ◽  
Jingyuan Li ◽  
Xia Han ◽  
Jinzhi He ◽  
...  
Keyword(s):  
Epigenetic Regulation ◽  
Root Development ◽  
Physiological Function ◽  
Tooth Root ◽  
Tooth Regeneration ◽  
Jaw Bones ◽  
Antagonistic Interaction ◽  
Tooth Type ◽  
Epigenetic Regulators

Patterning is a critical step during organogenesis and is closely associated with the physiological function of organs. Tooth root shapes are finely tuned to provide precise occlusal support to facilitate the function of each tooth type. However, the mechanism regulating tooth root patterning and development is largely unknown. In this study, we provide the first in vivo evidence demonstrating that Ezh2 in the dental mesenchyme determines patterning and furcation formation during dental root development in mouse molars. Mechanistically, an antagonistic interaction between epigenetic regulators Ezh2 and Arid1a controls Cdkn2a expression in the dental mesenchyme to regulate dental root patterning and development. These findings indicate the importance of balanced epigenetic regulation in determining the tooth root pattern and the integration of roots with the jaw bones to achieve physiological function. Collectively, our study provides important clues about the regulation of organogenesis and has general implications for tooth regeneration in the future.

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