scholarly journals Bone and Periodontal Tissue Regeneration by the Secretomes from Bone Marrow Derived Mesenchymal Stem Cells

10.5772/58907 ◽  
2014 ◽  
Author(s):  
Wataru Katagiri
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Periodontal Tissue ◽  
Periodontal Tissue Regeneration

Experimental Formation of Periodontal Structure Around Titanium Implants Utilizing Bone Marrow Mesenchymal Stem Cells: A Pilot Study

2009 ◽  
Vol 35 (3) ◽  
pp. 106-129 ◽  
Author(s):  
Mona K. Marei ◽  
Manal M. Saad ◽  
Adham M. El-Ashwah ◽  
Rania M. El-Backly ◽  
Mohammed A. Al-Khodary
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Dental Implants ◽  
Tissue Regeneration ◽  
Titanium Implants ◽  
Autogenous Bone ◽  
Periodontal Tissue ◽  
Periodontal Tissue Regeneration ◽  
Experimental Side

Abstract Tissue engineering in the head and neck area, presents numerous advantages. One of the most remarkable advantages is that regeneration of only a small amount of tissue can be highly beneficial to the patient, particularly in the field of periodontal tissue regeneration. For decades, successful osseointegration has provided thousands of restorations that maintain normal function. With the increasing need to utilize dental implants for growing patients and enhance their function to simulate normal tooth physiology and proprioception, there appears to be an urgent need for the concept of periodontal tissue regeneration around dental implants. In the present work, 5 goats were used for immediate implant placement post canine teeth extraction. Each goat received 2 implant fixtures; the control side received a porous hollow root-form poly (DL-Lactide-co-Glycolide) scaffold around the titanium fixture, and the experimental side received the same scaffold but seeded with autogenous bone marrow–derived mesenchymal stem cells. One animal was killed 10 days postoperatively, and the others were killed after 1 month. The results showed that on the experimental side, periodontal-like tissue with newly formed bone was demonstrated both at 10 days and after 1 month, while the control specimens showed early signs of connective tissue regeneration around the titanium fixture at 10 days, but was not shown in the 1 month specimens. It can be concluded that undifferentiated mesenchymal stem cells were capable of differentiating to provide the 3 critical tissues required for periodontal tissue regeneration: cementum, bone, and periodontal ligament. This work may provide a new approach for periodontal tissue regeneration.

Enhancement of Periodontal Tissue Regeneration by Transplantation of Bone Marrow Mesenchymal Stem Cells

2004 ◽  
Vol 75 (9) ◽  
pp. 1281-1287 ◽  
Author(s):  
Hiroyuki Kawaguchi ◽  
Akio Hirachi ◽  
Naohiko Hasegawa ◽  
Tomoyuki Iwata ◽  
Hidenori Hamaguchi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Periodontal Tissue ◽  
Periodontal Tissue Regeneration ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Lin Liu ◽  
Kun Liu ◽  
Yanzhe Yan ◽  
Zhuangzhuang Chu ◽  
Yi Tang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
Periodontal Tissue ◽  
Alizarin Red ◽  
Differentiation Potential ◽  
Alp Activity ◽  
Periodontal Tissue Regeneration

Objectives. Enhanced migration and osteogenic differentiation of mesenchymal stem cells (MSCs) are beneficial for MSC-mediated periodontal tissue regeneration, a promising method for periodontitis treatment. FBXO5, a member of the F-box protein family, is involved in the osteogenic differentiation of MSCs. Here, we investigated the effect of FBXO5 on human periodontal ligament stem cells (hPDLSCs). Materials and Methods. hPDLSCs were isolated from periodontal ligament tissue. Lentivirus FBXO5 shRNA was used to silence FBXO5 expression. Two transcripts of FBXO5 were overexpressed and transduced into hPDLSCs via retroviral infection. Migration and osteogenic differentiation of hPDLSCs were evaluated using the scratch migration assay, alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, western blotting, and real-time polymerase chain reaction. Results. The expression of FBXO5 was upregulated after osteogenic induction in hPDLSCs. FBXO5 knockdown attenuated migration, inhibited ALP activity and mineralization, and decreased RUNX2, OSX, and OCN expression, while the overexpression of two transcript isoforms significantly accelerated migration, enhanced ALP activity and mineralization, and increased RUNX2, OSX, and OCN expression in hPDLSCs. Conclusions. Both isoforms of FBXO5 promoted the migration and osteogenic differentiation potential of hPDLSCs, which identified a potential target for improving periodontal tissue regeneration.

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