scholarly journals Adipose Derived Stem Cells: Current State of the Art and Prospective Role in Regenerative Medicine and Tissue Engineering

10.5772/55924 ◽  
2013 ◽  
Author(s):  
Vincenzo Vindigni ◽  
Giorgio Giatsidis ◽  
Francesco Reho ◽  
Erica Dalla Venezia ◽  
Marco Mammana ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
State Of The Art ◽  
Adipose Derived Stem Cells ◽  
Current State

scholarly journals Stem Cells in Equine Veterinary Practice—Current Trends, Risks, and Perspectives

2019 ◽  
Vol 8 (5) ◽  
pp. 675
Author(s):  
Katarzyna Kornicka ◽  
Florian Geburek ◽  
Michael Röcken ◽  
Krzysztof Marycz
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Clinical Application ◽  
Scientific Community ◽  
Adipose Derived Stem Cells ◽  
Special Issue ◽  
Veterinary Practice ◽  
Current Trends

With this Editorial, we introduce the Special Issue “Adipose-Derived Stem Cells and Their Extracellular Microvesicles (ExMVs) for Tissue Engineering and Regenerative Medicine Applications” to the scientific community. In this issue, we focus on regenerative medicine, stem cells, and their clinical application.

scholarly journals Generation of an azide-modified extracellular matrix by adipose-derived stem cells using metabolic glycoengineering

2019 ◽  
Vol 5 (1) ◽  
pp. 393-395 ◽  
Author(s):  
Svenja Nellinger ◽  
Silke Keller ◽  
Alexander Southan ◽  
Valentin Wittmann ◽  
Ann-Cathrin Volz ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Functional Groups ◽  
Click Reaction ◽  
Fluorescent Dye ◽  
Adipose Derived Stem Cells ◽  
Metabolic Glycoengineering

AbstractNatural extracellular matrix (ECM) represents an ideal biomaterial for tissue engineering and regenerative medicine approaches. For further functionalization, there is a need for specific addressable functional groups within this biomaterial. Metabolic glycoengineering (MGE) provides a technique to incorporate modified monosaccharide derivatives into the ECM during their assembly, which was shown by us earlier for the production of a modified fibroblast-derived dermal ECM. In this study, adipose-derived stem cells (ASCs) were treated with the azide-modified monosaccharide derivate 1,3,4,6-tetra-O-acetyl-N-azidoacetylgalactosamine (Ac4GalNAz). Toxicity and viability assays after 24 h and 72 h incubation revealed high biocompatibility of Ac4GalNAz in contact with ASCs. The successful incorporation of the functional azide groups into the glycocalyx and the ECM of the ASCs was proven by conjugation with a fluorescent dye via a copper-catalyzed click reaction. Thus, Ac4GalNAz in combination with ASCs was confirmed to achieve an azidemodified ECM as a multifunctional biomaterial for further applications.

The role of adipose derived stem cells, smooth muscle cells and low intensity laser irradiation (LILI) in tissue engineering and regenerative medicine

2013 ◽  
Vol 8 (4) ◽  
pp. 331-336
Author(s):  
Bernard Mvula ◽  
Heidi Abrahamse
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Regenerative Medicine ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells ◽  
Low Intensity ◽  
Intensity Laser

AbstractTissue engineering and regenerative medicine has become the treatment of choice for several degenerative diseases. It involves the repairing or replacing of diseased or damaged cells or tissues. Stem cells have a key role to play in this multidisciplinary science because of their capacity to differentiate into several lineages. Adipose derived stem cells (ADSCs) are adult mesenchymal stem cells that are easily harvested and have the capacity to differentiate into cartilage, bone, smooth muscle, fat, liver and nerve cells. ADSCs have been found to differentiate into smooth muscle cells which play major roles in diseases such as asthma, hypertension, cancer and arteriosclerosis. Low Intensity Laser Irradiation (LILI), which involves the application of monochromatic light, has been found to increase viability, proliferation and differentiation in several types of cells including ADSCs. This review discusses the role of ADSCs, smooth muscle cells and LILI in the science of tissue engineering and regenerative medicine.

scholarly journals Adipose-Derived Stem Cells for Tissue Engineering and Regenerative Medicine Applications

2016 ◽  
Vol 2016 ◽  
pp. 1-19 ◽  
Author(s):  
Ru Dai ◽  
Zongjie Wang ◽  
Roya Samanipour ◽  
Kyo-in Koo ◽  
Keekyoung Kim
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells ◽  
Tissue Engineering Scaffolds ◽  
Stem Cell Source ◽  
Proliferation And Differentiation ◽  
Mesenchymal Stem Cell Source

Adipose-derived stem cells (ASCs) are a mesenchymal stem cell source with properties of self-renewal and multipotential differentiation. Compared to bone marrow-derived stem cells (BMSCs), ASCs can be derived from more sources and are harvested more easily. Three-dimensional (3D) tissue engineering scaffolds are better able to mimic thein vivocellular microenvironment, which benefits the localization, attachment, proliferation, and differentiation of ASCs. Therefore, tissue-engineered ASCs are recognized as an attractive substitute for tissue and organ transplantation. In this paper, we review the characteristics of ASCs, as well as the biomaterials and tissue engineering methods used to proliferate and differentiate ASCs in a 3D environment. Clinical applications of tissue-engineered ASCs are also discussed to reveal the potential and feasibility of using tissue-engineered ASCs in regenerative medicine.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

Tendon Tissue Engineering: Mechanism and Effects of Human Tenocyte Coculture With Adipose-Derived Stem Cells

2018 ◽  
Vol 43 (2) ◽  
pp. 183.e1-183.e9 ◽  
Author(s):  
Chao Long ◽  
Zhen Wang ◽  
Anais Legrand ◽  
Arhana Chattopadhyay ◽  
James Chang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering

scholarly journals Epithelial differentiation of adipose-derived stem cells for laryngeal tissue engineering

2009 ◽  
Vol 120 (1) ◽  
pp. 125-131 ◽  
Author(s):  
Jennifer L. Long ◽  
Patricia Zuk ◽  
Gerald S. Berke ◽  
Dinesh K. Chhetri
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Epithelial Differentiation ◽  
Adipose Derived Stem Cells

Regenerative Medicine: Applications and Development in Urology

2007 ◽  
Vol 74 (4) ◽  
pp. 197-205
Author(s):  
F. Pinto ◽  
A. Calarco ◽  
A. Brescia ◽  
E. Sacco ◽  
A. D'addessi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Regenerative Medicine ◽  
Cell Transplantation ◽  
Materials Science ◽  
Experimental Studies ◽  
Embryonic Stem ◽  
Human Embryos ◽  
Engineering Applications

Purpose Congenital abnormalities and acquired disorders can lead to organ damage and loss. Nowadays, transplantation represents the only effective treatment option. However, there is a marked decrease in the number of organ donors, which is even yearly worsening due to the population aging. The regenerative medicine represents a realistic option that allows to restore and maintain the normal functions of tissues and organs. This article reviews the principles of regenerative medicine and the recent advances with regard to its application to the genitourinary tract. Recent findings The field of regenerative medicine involves different areas of technology, such as tissue engineering, stem cells and cloning. Tissue engineering involves the field of cell transplantation, materials science and engineering in order to create functional replacement tissues. Stem cells and cloning permit the extraction of pluripotent, embryonic stem cells offering a potentially limitless source of cells for tissue engineering applications. Most current strategies for tissue engineering depend upon a sample of autologous cells from the patient's diseased organ. Biopsies from patients with extensive end-stage organ failure, however, may not yield enough normal cells. In these situations, stem cells are envisaged as being an alternative source. Stem cells can be derived from discarded human embryos (human embryonic stem cells), from fetal tissue or from adult sources (bone marrow, fat, skin). Therapeutic cloning offers a potentially limitless source of cells for tissue engineering applications. Regenerative medicine and tissue engineering scientists have increasingly applied the principles of cell transplantation, materials science and bioengineering to construct biological substitutes that will restore and maintain normal function in urological diseased and injured tissues such as kidney, ureter, bladder, urethra and penis. Conclusions Regenerative medicine offers several applications in acquired and congenital genitourinary diseases. Tissue engineering, stem cells and, mostly, cloning have been applied in experimental studies with excellent results. Few preliminary human applications have been developed with promising results.

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