scholarly journals Microbial Hydrocarbon Degradation: Efforts to Understand Biodegradation in Petroleum Reservoirs

10.5772/55920 ◽  
2013 ◽  
Author(s):  
Isabel Natalia ◽  
Valria Maia de Oliveir
Keyword(s):  
Petroleum Reservoirs ◽  
Hydrocarbon Degradation

Hydrocarbon Degradation in Petroleum Reservoirs

2010 ◽  
pp. 3097-3109 ◽  
Author(s):  
I. M. Head ◽  
S. R. Larter ◽  
N. D. Gray ◽  
A. Sherry ◽  
J. J. Adams ◽  
...  
Keyword(s):  
Petroleum Reservoirs ◽  
Hydrocarbon Degradation

scholarly journals Identify the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in oil and aqueous phases of petroleum reservoirs with different temperatures from China

2019 ◽  
Author(s):  
Zhichao Zhou ◽  
Bo Liang ◽  
Li-Ying Wang ◽  
Jin-Feng Liu ◽  
Bo-Zhong Mu ◽  
...  
Keyword(s):  
Oil Recovery ◽  
Petroleum Reservoirs ◽  
Environmental Benefits ◽  
Hydrocarbon Degradation ◽  
Water Phase ◽  
Temperature Phase ◽  
Fermentative Bacteria ◽  
Bacterial Microbiome ◽  
Two Phases ◽  
Microbial Groups

Abstract. Microorganisms in petroleum reservoirs mediate hydrocarbon degradation coupling with methanogenesis process as the terminal step, they also contribute to microbial enhanced oil recovery (MEOR) worldwide with great economic and environmental benefits. Here, a molecular investigation, using the 16S rRNA and mcrA gene profiles based on MiSeq sequencing and clone library construction method, was conducted on oil and water (aqueous) phases of samples with high, moderate and low temperatures from seven petroleum reservoirs in China. A core bacterial microbiome with a small proportion of shared OUT, but a high proportion of sequences among all reservoirs was discovered, including aerobic degraders, sulfate/nitrate reducing bacteria, fermentative bacteria and sulfur cycling bacteria distributed mainly in Proteobacteria, Bacteroidetes, Deferribacteres, Deinococcus-Thermus, Firmicutes, Spirochaetae and Thermotogae. Their prevalence in the reported petroleum reservoirs and successive enrichment cultures suggest their common roles and functions involved in aliphatic and aromatic hydrocarbon degradation. Dominant methanogenesis process generally shifts from hydrogenotrophic pathway in water phase to acetoclastic pathway in oil phase in high temperature reservoirs, but the opposite is true for low temperature reservoirs. No differences were detected between two phases in moderate temperature reservoirs. Physiochemical factors, including pH, temperature, phase conditions, and nitrate, Mn2+, and Mg2+ concentrations are the main ones correlating to the microbial compositional and functional profiles significantly. LEfSe analysis shows distribution differences of microbial groups towards pH, temperature, and oil/water. Tax4Fun functional profiling indicates major functional metabolism differences between the two phases, including amino acids, hydrocarbons in the oil phase, and carbohydrates in the water phase.

scholarly journals New Hydrocarbon Degradation Pathways in the Microbial Metagenome from Brazilian Petroleum Reservoirs

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e90087 ◽  
Author(s):  
Isabel Natalia Sierra-García ◽  
Javier Correa Alvarez ◽  
Suzan Pantaroto de Vasconcellos ◽  
Anete Pereira de Souza ◽  
Eugenio Vaz dos Santos Neto ◽  
...  
Keyword(s):  
Petroleum Reservoirs ◽  
Hydrocarbon Degradation ◽  
Degradation Pathways

scholarly journals Identifying the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in the oil and aqueous phases of petroleum reservoirs of different temperatures from China

2019 ◽  
Vol 16 (21) ◽  
pp. 4229-4241
Author(s):  
Zhichao Zhou ◽  
Bo Liang ◽  
Li-Ying Wang ◽  
Jin-Feng Liu ◽  
Bo-Zhong Mu ◽  
...  
Keyword(s):  
Aqueous Phase ◽  
Oil Recovery ◽  
Petroleum Reservoirs ◽  
Environmental Benefits ◽  
Hydrocarbon Degradation ◽  
Temperature Phase ◽  
Linear Discriminant ◽  
Fermentative Bacteria ◽  
Bacterial Microbiome ◽  
Two Phases

Abstract. Microorganisms in petroleum reservoirs play significant roles in hydrocarbon degradation, and through the terminal electron-accepting process of methanogenesis, they also contribute to microbially enhanced oil recovery (MEOR) worldwide, with great economic and environmental benefits. Here, a molecular investigation, using the 16S rRNA and mcrA gene profiles based on MiSeq sequencing and clone library construction methods, was conducted on oil and water (aqueous) phases of samples of high (82–88 ∘C), moderate (45–63 ∘C), and low temperatures (21–32 ∘C) from seven petroleum reservoirs in China. A core bacterial microbiome with a small proportion of shared operational taxonomic unit (OTU) values, but a high proportion of sequences among all reservoirs was discovered, including aerobic degraders, sulfate- and nitrate-reducing bacteria, fermentative bacteria, and sulfur-oxidizing bacteria distributed mainly in Proteobacteria, Bacteroidetes, Deferribacteres, Deinococcus–Thermus, Firmicutes, Spirochaetes, and Thermotogae. Their prevalence in the previously reported petroleum reservoirs and successive enrichment cultures suggests their common roles and functions involved in aliphatic and aromatic hydrocarbon degradation. The methanogenic process generally shifts from the dominant hydrogenotrophic pathway in the aqueous phase to the acetoclastic pathway in the oil phase in high-temperature reservoirs, but the opposite was true for low-temperature reservoirs. No difference was detected between the two phases in moderate temperature reservoirs. Physicochemical factors, including pH; temperature; phase conditions; and nitrate, Mn2+, and Mg2+ concentrations were the main factors correlated to the microbial compositional and functional profiles significantly. Linear discriminant analysis (LDA) effect size (LEfSe) analysis shows distribution differences of microbial groups towards pH, temperature, and the oil and aqueous phases. Using the software Tax4Fun for functional profiling indicated functional metabolism differences between the two phases, including amino acids, hydrocarbons in the oil phase, and carbohydrates in the aqueous phase.

NUMERICAL FLOW SIMULATION IN NATURALLY FRACTURED PETROLEUM RESERVOIRS USING A MPFA-STREAMLINE FORMULATION

2019 ◽  
Author(s):  
Kelly Cristinne Leite Angelim ◽  
Túlio Cavalcante ◽  
Jonathan Teixeira ◽  
Paulo Roberto Maciel Lyra ◽  
DARLAN KARLO ELISIÁRIO DE CARVALHO
Keyword(s):  
Flow Simulation ◽  
Petroleum Reservoirs ◽  
Naturally Fractured ◽  
Numerical Flow Simulation
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