scholarly journals The Role of Immature Granulocyte Count and Immature Myeloid Information in the Diagnosis of Neonatal Sepsis

10.5772/54531 ◽  
2013 ◽  
Author(s):  
Christina Cimenti ◽  
Wolfgang Erwa ◽  
Wilhelm Muller ◽  
Bernhard Resch
Keyword(s):  
Neonatal Sepsis ◽  
Granulocyte Count ◽  
Immature Granulocyte

scholarly journals Role of Manual Immature to Total Neutrophil (I/T) Ratio and Automated Immature Granulocyte Count (IGC) and Percentage (IG%) in the Early Diagnosis of Neonatal Sepsis

2021 ◽  
Author(s):  
Nalika Jayasekara ◽  
Chandima Kulathilake ◽  
Saraji Wijesekara ◽  
Indira Wijesiriwardena
Keyword(s):  
Neonatal Sepsis ◽  
Cross Sectional Study ◽  
Reference Intervals ◽  
Full Blood Count ◽  
Cross Sectional ◽  
Granulocyte Count ◽  
Reactive Protein ◽  
Immature Granulocyte ◽  
Low Sensitivity

Abstract Background: The diagnosis of neonatal sepsis is challenging due to non-specific and subtle clinical features, low sensitivity and delay in routine laboratory tests. Current study was conducted to evaluate the role of manual immature/total (I/T) neutrophil ratio and automated immature granulocyte count (IGC) and immature granulocyte percentage (IG%) in the diagnosis of neonatal sepsis. Materials and Methods: An analytical cross-sectional study was done during a period of 6 months with a sample of 55 neonates admitted to Colombo South Teaching Hospital, Sri Lanka. A combination of clinical and laboratory parameters including full blood count, C-reactive protein and blood culture were used to identify the neonates with probable sepsis. The population was subcategorized into five (5) groups and manual immature/total neutrophil (I/T) ratio, immature granulocyte count (IGC) and immature granulocyte%(IG%) were done in each neonate. Results: The sensitivity of manual I/T ratio was 93.75% and negative predictive value (NPV) was 95.24%. The sensitivity for lower cut off values, IGC of 0.03x103/µL and IG% of 0.5% was 80% and 73.33% respectively. The NPV for above cut-off values were 25% and 0.5% respectively. The NPV was improved with higher cut-off values with 70.90% for IGC 0.3 and 70.59% for IG 3%, but sensitivity remained low with 40% and 33.33% respectively.Conclusion: Manual I/T ratio remains as a useful diagnostic tool in diagnosing and excluding neonatal sepsis with a very good sensitivity and NPV. However, further studies and well defined reference intervals are required in automated IGC and IG%.

scholarly journals Performance of an Automated Immature Granulocyte Count as a Predictor of Neonatal Sepsis

2005 ◽  
Vol 123 (4) ◽  
pp. 618-624 ◽  
Author(s):  
Kelly G. Nigro ◽  
MaryAnn O’Riordan ◽  
Eleanor J. Molloy ◽  
Michele C. Walsh ◽  
Linda M. Sandhaus
Keyword(s):  
Neonatal Sepsis ◽  
Granulocyte Count ◽  
Immature Granulocyte

The predictive value of immature granulocyte count and immature myeloid information in the diagnosis of neonatal sepsis

2012 ◽  
Vol 50 (8) ◽  
Author(s):  
Christina Cimenti ◽  
Wolfgang Erwa ◽  
Kurt R. Herkner ◽  
David C. Kasper ◽  
Wilhelm Müller ◽  
...  
Keyword(s):  
Neonatal Sepsis ◽  
Predictive Value ◽  
Granulocyte Count ◽  
Immature Granulocyte

scholarly journals ROLE OF IMMATURE/TOTAL NEUTROPHIL RATIO, LEUKOCYTE COUNT AND PROCALCITONIN IN DIAGNOSING NEONATAL SEPSIS

2018 ◽  
Vol 24 (2) ◽  
pp. 169
Author(s):  
I A Putri Wirawati1 ◽  
Aryati Aryati ◽  
A A Wiradewi Lestari
Keyword(s):  
Likelihood Ratio ◽  
Neonatal Sepsis ◽  
Leukocyte Count ◽  
Systemic Disease ◽  
Clinical Syndrome ◽  
Cross Sectional ◽  
Bacteria Growth ◽  
Long Time ◽  
Cross Sectional Design

Neonatal sepsis is a clinical syndrome of systemic disease, accompanied by bacteremia that occurs during infants in the first month of life. A late diagnosis might increase mortality. The presence of bacteria growth in blood cultures is a definitive diagnosis. Unfortunately, culture results are usually obtained of a long time. The study aimed to analyze sensitivity and specificity of the manual I/T ratio, automatic I/T ratio, leukocyte count and procalcitonin (PCT) to diagnose neonatal sepsis. This study used a cross-sectional design, from the NICU room in Sanglah General Hospital, Denpasar. There were 59 patients who met the study criteria. Along with blood culture as the gold standard in determining diagnosis of sepsis and with I/T ratio cut-off of 0.2, the sensitivity of manual I/T ratio was 69.2%, specificity 83.9%, PPV 63.9%, NPV 87% and likelihood ratio was 3.06. While the sensitivity of automatic I/T ratio was 47.6%, specificity 85.8%, PPV 55.1%, NPV 81.4% and likelihood ratio was 2.25. Based on the normal range of leukocyte count (9.1 - 34 x 103/μl), sensitivity of leukocyte count was 59%, specificity 71.5%, PPV 46.7%, NPV 80.9% and likelihood ratio was 1.59. With PCT cut-off 0.5 ng/mL, the obtained sensitivity of PCT was 64.3%, specificity 85.8%, PPV 64.3%, NPV 85.8% and likelihood ratio was 3.13. 

scholarly journals Clinical significance of miR-129-5p in patients with neonatal sepsis and its regulatory role in the LPS-induced inflammatory response

2020 ◽  
Author(s):  
Meng Li ◽  
Xiaoyang Huang ◽  
Qingcui Zhuo ◽  
Jinghui Zhang ◽  
Xiuli Ju
Keyword(s):  
Inflammatory Response ◽  
Clinical Significance ◽  
Neonatal Sepsis ◽  
Close Association ◽  
Disease Diagnosis ◽  
Diagnostic Value ◽  
Regulatory Role ◽  
Value Analysis ◽  
Tnf Α

Neonatal sepsis (NS) occurs in neonates within 28 days, especially preterm infants. The dysregulation of miRNAs is widely detected in NS. The study investigated the expression changes and clinical significance of miR-129-5p in NS patients and further explored the regulatory role of miR-129-5p in the LPS-induced inflammatory response in monocytes. A total of 75 neonates with NS and 84 neonates without NS were recruited. qRT-PCR was used for the measurement of miR-129-5p expression. The receiver operating characteristic (ROC) curve was constructed for diagnostic value analysis. ELISA was used to detect the concentration of inflammatory cytokines. Monocytes were isolated from the blood of neonates to investigate the role of miR-129-5p in the LPS-induced inflammatory response in vitro. miR-129-5p was low expressed in the serum of NS cases compared with controls. Serum miR-129-5p had a diagnostic value for NS with a sensitivity of 82.7% and specificity of 79.8%. There was close association for serum miR-129-5p with TNF-α (r = -0.652, p < 0.001) and IL-8 (r = -0.700, p < 0.001) levels in NS patients. Overexpression of miR-129-5p reversed the increasing trend of TNF-α and IL-8 induced by LPS, whereas miR-129-5p downregulation aggravated the increase of TNF-α and IL-8 induced by LPS in monocytes. MiR-129-5p was downregulated in the serum of NS patients, and it might be a promising biomarker for disease diagnosis. Overexpression of miR-129-5p alleviated the inflammatory response of NS.

Role of routine lumbar puncture in neonatal sepsis

1995 ◽  
Vol 31 (1) ◽  
pp. 8-10 ◽  
Author(s):  
P. KUMAR ◽  
S. SARKAR ◽  
A. NARANG
Keyword(s):  
Lumbar Puncture ◽  
Neonatal Sepsis

Granulocyte Transfusions

PEDIATRICS ◽  
1988 ◽  
Vol 81 (6) ◽  
pp. 915-915
Author(s):  
MITCHELL S. CAIRO
Keyword(s):  
Supportive Care ◽  
Preterm Infants ◽  
Clinical Setting ◽  
Neonatal Sepsis ◽  
Statistical Significance ◽  
Buffy Coat ◽  
Granulocyte Transfusion

In Reply.— The comments raised by Baley et al are well taken and bring up one of the most important variables in the role of granulocyte transfusion in neonatal sepsis. Most of the studies to date have involved small numbers of patients in single institutions. This has prevented us from accumulating a large enough population of patients to adequately assess the role of either buffy coat transfusions or leukapheresed transfusions in this clinical setting. My comments in the commentary suggested that, with only eight patients in a group of preterm infants with presumed sepsis and only five patients with presumed sepsis with birth weights greater than 1,500 g, the numbers are essentially too small to determine any statistical significance between the role of buffy coat transfusions or supportive care.

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