scholarly journals Phase and Polarization Contrast Methods by Use of Digital Holographic Microscopy: Applications to Different Types of Biological Samples

Author(s):  
Francisco Palacios ◽  
Oneida Font ◽  
Guillermo Palacios ◽  
Jorge Ricardo ◽  
Miriela Escobedo ◽  
...  
2010 ◽  
Vol 37 (11) ◽  
pp. 2906-2911 ◽  
Author(s):  
赵洁 Zhao Jie ◽  
王大勇 Wang Dayong ◽  
李艳 Li Yan ◽  
王云新 Wang Yunxin ◽  
张亦卓 Zhang Yizhuo

Author(s):  
А.В. Белашов ◽  
А.А. Жихорева

A novel method for the quantitative characterization of fixed histological samples based on the statistical analysis of their phase images obtained using digital holographic microscopy is developed and presented. The proposed approach allows for fully automated processing of reconstructed phase images and obtaining quantitative data of morphological and optical characteristics of histological tissues structures. The method was validated on three histological samples of different types of tissues: ciliated columnar epithelium, elastic cartilage, and liver.


2021 ◽  
Vol 84 ◽  
pp. 200-207
Author(s):  
Jiansen Pan ◽  
Qingmei Peng ◽  
Guoliang Zhang ◽  
Qingyi Xie ◽  
Xiangjun Gong ◽  
...  

Photonics ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 276
Author(s):  
Yun Liu ◽  
Peihua Bu ◽  
Mingxing Jiao ◽  
Junhong Xing ◽  
Ke Kou ◽  
...  

The coherent noise always exists in digital holographic microscopy due to the laser source, degrading the image quality. A method of speckle suppression using the dynamic speckle illumination, produced by double-moving diffusers, is presented in digital holographic microscopy. The space–time correlation functions are theoretically analyzed from the statistics distribution in the doubly and singly scattered system, respectively. The configuration of double-moving diffusers is demonstrated to have better performance in speckle suppression compared with the single diffuser and moving-static double diffusers cases. The experiment results verify the feasibility of the approach. The presented approach only requires a single shot interferogram to realize the speckle reduction, accordingly it has the potential application in real-time measurement.


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