scholarly journals Cellular Uptake Mechanism of Non-Viral Gene Delivery and Means for Improving Transfection Efficiency

10.5772/53353 ◽  
2013 ◽  
Author(s):  
Shengnan Xiang ◽  
Xiaoling Zhang
Keyword(s):  
Gene Delivery ◽  
Cellular Uptake ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Uptake Mechanism ◽  
Viral Gene Delivery ◽  
Cellular Uptake Mechanism

scholarly journals Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system

2020 ◽  
Vol 11 ◽  
pp. 1685-1692
Author(s):  
Hiroaki Komuro ◽  
Masahiro Yamazoe ◽  
Kosuke Nozaki ◽  
Akiko Nagai ◽  
Tetsuo Sasano
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Cell Uptake ◽  
Viral Gene ◽  
Target Cells ◽  
Gene Delivery System ◽  
Uptake Mechanism ◽  
Delivery Vector ◽  
Low Cytotoxicity ◽  
Viral Gene Delivery

Gene therapy has been explored as a future alternative for treating heart disease. Among several gene delivery systems aimed at penetrating specific target cells, we focused on safe and non-viral gene delivery materials with a high transfection efficiency. Although various techniques have been developed, the mechanisms underlying the cellular uptake of gene delivery materials have not yet been sufficiently studied in cardiomyocytes. The aim of this study was to determine how hydroxyapatite (HAp) nanoparticles contribute to the delivery of plasmid DNA (pDNA) into cardiomyocytes. We fabricated HAp nanoparticles using the water-in-oil (W/O) emulsion method and used these nanoparticles as the delivery vector for transfecting cardiomyocyte-derived HL-1 cells. HAp exhibited particles on the nanoscale and with a low cytotoxicity in HL-1 cells. The transfection assay performed with several endocytosis inhibitors suggested that the HAp/pDNA complexes were internalized by HL-1 cells through macropinocytosis. Furthermore, this HL-1 cell uptake was generated in response to HAp stimulation. Thus, HAp is a positive regulator of macropinocytosis in HL-1 cells and a good system for gene delivery in cardiomyocytes.

scholarly journals Non-viral Gene Therapy for Osteoarthritis

2021 ◽  
Vol 8 ◽  
Author(s):  
Ilona Uzieliene ◽  
Ursule Kalvaityte ◽  
Eiva Bernotiene ◽  
Ali Mobasheri
Keyword(s):  
Gene Therapy ◽  
Gene Delivery ◽  
Articular Chondrocytes ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Synovial Joint ◽  
Advantages And Disadvantages ◽  
Viral Gene Therapy ◽  
Viral Gene Delivery ◽  
Significant Effort

Strategies for delivering nucleic acids into damaged and diseased tissues have been divided into two major areas: viral and non-viral gene therapy. In this mini-review article we discuss the application of gene therapy for the treatment of osteoarthritis (OA), one of the most common forms of arthritis. We focus primarily on non-viral gene therapy and cell therapy. We briefly discuss the advantages and disadvantages of viral and non-viral gene therapy and review the nucleic acid transfer systems that have been used for gene delivery into articular chondrocytes in cartilage from the synovial joint. Although viral gene delivery has been more popular due to its reported efficiency, significant effort has gone into enhancing the transfection efficiency of non-viral delivery, making non-viral approaches promising tools for further application in basic, translational and clinical studies on OA. Non-viral gene delivery technologies have the potential to transform the future development of disease-modifying therapeutics for OA and related osteoarticular disorders. However, further research is needed to optimize transfection efficiency, longevity and duration of gene expression.

Decaarginine-PEG-Artificial Lipid/DNA Complex for Gene Delivery: Nanostructure and Transfection Efficiency

2008 ◽  
Vol 8 (5) ◽  
pp. 2308-2315 ◽  
Author(s):  
Masahiko Furuhata ◽  
Radostin Danev ◽  
Kuniaki Nagayama ◽  
Yoshifumi Yamada ◽  
Hiroko Kawakami ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cellular Uptake ◽  
Plasmid Dna ◽  
Fluorescent Protein ◽  
Transfection Efficiency ◽  
Distribution Analysis ◽  
Lipid Concentration ◽  
Uptake Mechanism ◽  
Transmission Electron ◽  
Green Fluorescent

Oligoarginine conjugates are highly efficient vectors for the delivery of plasmid DNA into cells. Decaarginine-conjugated lipid (Arg10-PEG-lipid) was synthesized and the effects of Arg10-PEG-lipid concentration at a fixed DNA concentration on transfection efficiency and the structure of the complexes were studied below and above critical micelle concentration (CMC), and at the lipid nitrogen/DNA phosphate (N/P) ratio corresponding to transfection, respectively. Arg10-PEG-lipid at the concentration below CMC showed stronger interaction with DNA by fluorescence intensity distribution analysis, and significantly higher luciferase and green fluorescent protein expression than that above CMC. A phase-contrast cryo-transmission electron microscope (cryo-TEM) experiment showed that the morphology of the complexes depended on the N/P ratio. At a low N/P ratio corresponding to that in transfection at a lipid concentration below CMC, a net-like structure developed in which plasmid DNA was involved. A further increase in the N/P ratio, a large fibrous nanostructure of complexes, was also observed. Without DNA, these structures were not obtained. The cellular uptake mechanism of complexes using flow cytometry with inhibitors suggested that complexes with two different morphologies showed similar cellular uptake and uptake mechanism, macropinocytosis. Differences in transfection efficiency of the complexes may be explained by a large fibrous nanostructure inhibiting the cellular internalization of complexes or the release of DNA from macropinosomes into cytoplasm. Arg10-PEG-lipid/DNA complexes formed a favorable nanostructure for gene delivery, depending on the N/P ratio in water.

Highly Branched Poly(β-amino esters) for Non-Viral Gene Delivery: High Transfection Efficiency and Low Toxicity Achieved by Increasing Molecular Weight

2016 ◽  
Vol 17 (11) ◽  
pp. 3640-3647 ◽  
Author(s):  
Yongsheng Gao ◽  
Jian-Yuan Huang ◽  
Jonathan O’Keeffe Ahern ◽  
Lara Cutlar ◽  
Dezhong Zhou ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Amino Esters ◽  
High Transfection Efficiency ◽  
Low Toxicity ◽  
Viral Gene Delivery
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