scholarly journals Experimental Requirements for in vitro Studies Aimed to Evaluate the Biological Effects of Radiofrequency Radiation

10.5772/51421 ◽  
2012 ◽  
Author(s):  
Olga Zeni ◽  
Maria Rosaria
Keyword(s):  
Biological Effects ◽  
In Vitro Studies ◽  
Radiofrequency Radiation

In vitro studies of biological effects of cigarette smoke condensate

1985 ◽  
Vol 157 (2-3) ◽  
pp. 169-180 ◽  
Author(s):  
Margareta Curvall ◽  
Tommy Jansson ◽  
Bertil Pettersson ◽  
Annica Hedin ◽  
Curt R. Enzell
Keyword(s):  
Cigarette Smoke ◽  
Biological Effects ◽  
In Vitro Studies ◽  
Cigarette Smoke Condensate

In vitro studies of biological effects of cigarette smoke condensate

1986 ◽  
Vol 169 (3) ◽  
pp. 129-139 ◽  
Author(s):  
Tommy Jansson ◽  
Margareta Curvall ◽  
Annica Hedin ◽  
Curt R. Enzell
Keyword(s):  
Cigarette Smoke ◽  
Biological Effects ◽  
In Vitro Studies ◽  
Cigarette Smoke Condensate

Exosome-Transferred APOC3-Ncrna Complex Mediates Iron Regulation in Beta-Thalassemia Major

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 963-963
Author(s):  
Ching-Tien Peng
Keyword(s):  
Conflicts Of Interest ◽  
Ex Vivo ◽  
Biological Effects ◽  
Thalassemia Major ◽  
In Vitro Studies ◽  
Luciferase Assay ◽  
Iron Regulation ◽  
Beta Thalassemia ◽  
Western Blots

Background: Exosomes are small membrane vesicles (50-90 mm in diameter) containing bioactive proteins and genetic materials that may be transferred to accept cells, resulting in potent biological effects in the circulatory system. Exosome-transferred APOC3-ncRNA may be a crucial function in iron regulation. However, the role in β-thalassemia major is remains unclear. Aim: The aim of this study was to investigate how Exosome-transferred APOC3-ncRNA adapts to iron regulation in β-thalassemia major. Design: Using Proteomics, RNA-sequencing and lncRNA Q-PCR array, we demonstrated expression of exosomes-transferred APOC3-ncRNAs in plasma of β-thalassemia major patients (n = 40). Identification of exosome by Dynamic light scattering (DLS), Flow cytometry and western blots. Lentiviral infection, ncRNAs transfection and in vitro studies revealed that knockdown or overexpression obviously increased or ameliorated exosomes-induced iron regulation. Bioinformatics analysis, luciferase assay and in vitro studies revealed that ncRNAs functioned as a repressing mediator and formed a feedback loop with ncRNAs and target gene to mediate iron regulation. Results: We observed that ncRNA is differential expressed in the exosomes of β-thalassemia major. We analyzed the effects of ex vivo-derived exosomes for iron regulation by monocytes/hepatocytes model. In vitro model showed that exosomes were internalized by THP-1/HuH-7 cells, and regulates the IRP1/FPN/HAMP. In turn, Exosome-transferred APOC3-ncRNA mediates inflammatory cytokine expression and iron regulation via p38/pAKT/TRAF6/NF-kB pathway. Knockdown of APOC3-ncRNA reversed IRP1/FPN/HAMP expression. Conclusions: This is the first example of extracellular APOC3/ncRNA regulating gene expression via circulatory exosomes-transferred to accept cells. Increased Exosome-transferred APOC3-ncRNA expression is one of the key factors that might have contributed to abnormal iron regulation in β-thalassemia major patients. Disclosures No relevant conflicts of interest to declare.

scholarly journals Influence of Angiotensin II on cell viability and apoptosis in rat renal proximal tubular epithelial cells in in vitro studies

2020 ◽  
Vol 21 (3) ◽  
pp. 147032032094985
Author(s):  
Aleksandra Piotrowska ◽  
Magdalena Chmielewska ◽  
Waldemar Andrzejewski ◽  
Piotr Dziegiel ◽  
Marzenna Podhorska-Okolow
Keyword(s):  
Angiotensin Ii ◽  
Cell Viability ◽  
Cell Line ◽  
Biological Effects ◽  
In Vitro Studies ◽  
Ang Ii ◽  
Western Blot Method ◽  
Proximal Tubular Epithelial Cells ◽  
Mtt Tests

Introduction: Angiotensin II (Ang II) is multifunctional peptide that plays an important role in blood pressure regulation and maintenance electrolyte homeostasis. It shows biological effects by activating two main receptors: AT1 and AT2. The aim of the present work was to investigate the effect of Ang II on NRK-52E cells in in vitro studies. Furthermore, an attempt was made to determine the effectiveness of the AT1 and AT2 receptor blocker activity (respectively, losartan and PD123319). Methods: The study was carried out using adherent NRK-52E cell line. Immunofluorescence and Western Blot method were used to confirm the presence of AT1 and AT2 receptors in the cells. The SRB and MTT tests showed decrease in the viability of NRK-52E cells incubated with Ang II in comparison to the control (without Ang II). Results: The blockade of the AT1 receptor caused an increase in cell viability in comparison to cells incubated with Ang II only. The blockade of AT2 receptor also triggered statistically significant increase in cell viability in comparison with cells only exposed to Ang II. Combined administration of blockers for both receptors (losartan and PD123319) decreased Ang II cytotoxicity against NRK-52E cell line. The apoptosis was only observed in cells incubated with Ang II in comparison with control cells. However, simultaneous use of both blockers caused statistically significant decrease in apoptosis. Conclusions: The result of our study indicates that Ang II causes damaging effect on NRK-52E cells by directing them to programmed cell death. It seems that not only does the AT2 receptor itself play an important role in the induction of apoptosis, but also its interaction with AT1 receptor does as well.

Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

1972 ◽  
Vol 30 ◽  
pp. 350-351
Author(s):  
K. Shankar Narayan ◽  
Kailash C. Gupta ◽  
Tohru Okigaki
Keyword(s):  
Ultraviolet Light ◽  
Mammalian Cells ◽  
Biological Effects ◽  
Survival Rates ◽  
Chinese Hamster ◽  
Cell Types ◽  
Differential Sensitivity ◽  
Ultrastructural Changes ◽  
Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production
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