scholarly journals Molecular Detection and Genotyping of Toxoplasma gondii from Clinical Samples

10.5772/50830 ◽  
2012 ◽  
Author(s):  
Vladimir Ivovic ◽  
Marija Vujanic ◽  
Tijana Zivkovic ◽  
Ivana Klun ◽  
Olgica Djurkovic-Djakovic
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Clinical Samples

scholarly journals Molecular detection of Brucella spp. from broth culture of clinical samples in Nigeria: Its role in vaccine quality control

2009 ◽  
Vol 8 (21) ◽  
pp. 5661-5665 ◽  
Author(s):  
F Antiabong J ◽  
Yakubu B ◽  
A Owolodun O ◽  
Bertu W ◽  
A Ocholi R
Keyword(s):  
Quality Control ◽  
Molecular Detection ◽  
Broth Culture ◽  
Clinical Samples

scholarly journals Performance of 2 Polymerization Protocols Targeting Cloned Toxoplasma Parasites

2018 ◽  
Vol 6 (9) ◽  
pp. 1577-1580
Author(s):  
Nihal A. Hanafy ◽  
Mohamed S. Badr ◽  
Ghada M. Nasr
Keyword(s):  
Toxoplasma Gondii ◽  
Real Time ◽  
Parasitic Infection ◽  
Clinical Samples ◽  
Molecular Assays ◽  
Low Concentrations ◽  
Target Dna ◽  
Primer Sets ◽  
Amplification Failure ◽  
Reference Samples

BACKGROUND: Toxoplasma gondii is a common parasitic infection of humans. Infection is usually mild. Serious complications can occur in pregnant and immunocompromised patients. AIM: The present study aims to investigate the performance of 2 different PCR protocols; real-time quantitative molecular assays (qPCR) and conventional molecular assays (cPCR), using 2 different sets of primers and by using cloned purified Toxoplasma genomic substances to be evaluated as reference samples. METHODS: The target DNA was provided in 8 different quantities. RESULTS: Amplification failure was reported only with the cPCR in samples of low concentrations using both primer sets. Quantitative PCR detected the 8 different dilutions of the purified Toxoplasma gondii using the 2 sets of primers while cPCR was sensitive to detect only 6 different dilutions. CONCLUSION: Generally real-time quantitative molecular assays, is easy to use method compared to conventional PCR assay and produces more reliable results within only one hour time but still the possible application of qPCRs in routine diagnosis necessitates analysis of a large number of clinical samples in further studies to make the proper choice.

Molecular Detection and Genotyping of Toxoplasma gondii in Edward's Long-Tailed Rats (Leopoldamys edwardsi)

2019 ◽  
Vol 16 (8) ◽  
pp. 539-542
Author(s):  
Wen-Bin Zheng ◽  
Bin-Ze Gui ◽  
Hai-Bin Long ◽  
Yi-Wei Chen ◽  
Xing-Quan Zhu ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection

Molecular Detection of Toxoplasma gondii in the Slaughter Sheep and Goats from Shandong Province, Eastern China

2020 ◽  
Vol 20 (3) ◽  
pp. 193-196 ◽  
Author(s):  
Kang Ai ◽  
Cui-Qin Huang ◽  
Jing-Jing Guo ◽  
Hua Cong ◽  
Shen-Yi He ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Eastern China ◽  
Shandong Province ◽  
Sheep And Goats

scholarly journals Molecular detection of Toxoplasma gondii in house sparrow (Passer domesticus) by LAMP and PCR methods in Tehran, Iran

2015 ◽  
Vol 40 (4) ◽  
pp. 1317-1321 ◽  
Author(s):  
Amir Abdoli ◽  
Abdolhossein Dalimi ◽  
Haleh Soltanghoraee ◽  
Fatemeh Ghaffarifar
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
House Sparrow ◽  
Passer Domesticus

Molecular detection of Toxoplasma gondii in the slaughter sheep and goats from North India

2017 ◽  
Vol 241 ◽  
pp. 35-38 ◽  
Author(s):  
Deepali Kalambhe ◽  
J.P.S. Gill ◽  
Balbir Bagicha Singh
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
North India ◽  
Sheep And Goats
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