scholarly journals Exploring Surface Display Technology for Enhancement of Delivering Viable Lactic Acid Bacteria to Gastrointestinal Tract

10.5772/50783 ◽  
2013 ◽  
Author(s):  
Shirin Tarahomjoo
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Surface Display ◽  
Display Technology

scholarly journals A SH3_5 Cell Anchoring Domain for Non-recombinant Surface Display on Lactic Acid Bacteria

2021 ◽  
Vol 8 ◽  
Author(s):  
Pei Kun Richie Tay ◽  
Pei Yu Lim ◽  
Dave Siak-Wei Ow
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Surface Display ◽  
Bioactive Molecules ◽  
Gastric Digestion ◽  
Functional Protein ◽  
Fluorescent Reporter ◽  
Surface Conditions ◽  
Bacterial Surface

Lactic acid bacteria (LAB) are a group of gut commensals increasingly recognized for their potential to deliver bioactive molecules in vivo. The delivery of therapeutic proteins, in particular, can be achieved by anchoring them to the bacterial surface, and various anchoring domains have been described for this application. Here, we investigated a new cell anchoring domain (CAD4a) isolated from a Lactobacillus protein, containing repeats of a SH3_5 motif that binds non-covalently to peptidoglycan in the LAB cell wall. Using a fluorescent reporter, we showed that C-terminal CAD4a bound Lactobacillus fermentum selectively out of a panel of LAB strains, and cell anchoring was uniform across the cell surface. Conditions affecting CAD4a anchoring were studied, including temperature, pH, salt concentration, and bacterial growth phase. Quantitative analysis showed that CAD4a allowed display of 105 molecules of monomeric protein per cell. We demonstrated the surface display of a functional protein with superoxide dismutase (SOD), an antioxidant enzyme potentially useful for treating gut inflammation. SOD displayed on cells could be protected from gastric digestion using a polymer matrix. Taken together, our results show the feasibility of using CAD4a as a novel cell anchor for protein surface display on LAB.

scholarly journals Activity and viability of probiotic candidates consisting of lactic acid bacteria and yeast isolated from native poultry gastrointestinal tract

2018 ◽  
Author(s):  
Rima Shidqiyya Hidayati Martin ◽  
Erika Budiarti Laconi ◽  
Anuraga Jayanegara ◽  
Ahmad Sofyan ◽  
Lusty Istiqomah
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria

scholarly journals Isolation, identification and potential probiotic characterization of lactic acid bacteria from Thai traditional fermented food

2021 ◽  
Vol 7 (4) ◽  
pp. 431-446
Author(s):  
Sunisa Suwannaphan ◽  
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
High Surface ◽  
Surface Hydrophobicity ◽  
Fermented Food ◽  
Probiotic Properties ◽  
Two Samples ◽  
Traditional Fermented Food

<abstract> <p>The probiotic potential of lactic acid bacteria (LAB) isolated from Thai traditional fermented food was investigated. Forty-two samples were collected from four markets in Phra Nakhon Si Ayutthaya Province. Out of 50 isolated LAB, 6 (a3, f4, f8, K1, K4 and K9) obtained from pla-ra and bamboo shoot pickle samples showed high tolerance to gastrointestinal tract conditions. These isolates were selected to identify and characterize their probiotic properties. Isolate a3 was identified as <italic>Weissella thailandensis</italic>, isolates f4 and f8 were identified as belonging to <italic>Enterococcus thailandicus</italic> and isolates K1, K4 and K9 were determined as <italic>Limosilactobacillus fermentum</italic>. All six LAB exhibited high autoaggregation ability (93.40–95.01%), while <italic>W. thailandensis</italic> isolate a3 showed potential for coaggregation in almost all the pathogenic bacteria tested. Cell-free supernatant (CFS) obtained from all isolates did not inhibit <italic>Staphylococcus aureus</italic>. CFS derived from <italic>L. fermentum</italic> isolate K4 showed the most efficient antimicrobial activity, in particular against Gram-negative bacteria, while <italic>L. fermentum</italic> isolate K4 presented high surface hydrophobicity in the presence of xylene and n-hexane. All LAB isolates were found to be resistant to clindamycin and nalidixic acid, whereas <italic>E. thailandicus</italic> isolate f8 exhibited resistance to most of the antibiotics tested. <italic>L. fermentum</italic> isolate K4 showed promise as a suitable probiotic candidate for future applications in the food industry due to tolerance to gastrointestinal tract conditions with high surface hydrophobicity and inhibited most of the pathogens tested.</p> </abstract>

scholarly journals Isolation and characterization of lactic acid bacteria from fecal pellets, coelomic fluid, and gastrointestinal tract of Nypa worm (Namalycastis rhodochorde) from West Kalimantan, Indonesia

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
Ari Hepi Yanti ◽  
Tri Rima Setyawati ◽  
Rikhsan Kurniatuhadi
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Coelomic Fluid ◽  
Phenol Red ◽  
Cellulolytic Activity ◽  
Fecal Pellets ◽  
West Kalimantan ◽  
Isolation And Characterization

Abstract. Yanti AH, Setyawati TR, Kurniatuhadi R. 2020. Isolation and characterization of lactic acid bacteria from fecal pellets, coelomic fluid, and gastrointestinal tract of Nypa worm (Namalycastis rhodochorde) from West Kalimantan, Indonesia. Biodiversitas 21: 4726-4731. Lactic acid bacteria isolated from the intestinal tract as probiotic could be beneficial because they could trigger the growth of the host by enhancing digestibility, increasing their immune system, and inhibiting pathogenic bacteria through the enzymatic process or metabolites production. The purposes of this study were to identify and characterize lactic acid bacteria from fecal pellets, coelomic fluid, and gastrointestinal tract of Nypa worm (Namalycastis rhodochorde). Bacterial isolation was carried out by the pour plate method on de Mann Rogosa Sharp Agar (MRSA). Lactic Acid Bacteria (LAB) isolates were purified and then physiologically characterized by some biochemical tests. Cellulolytic activities were carried out by detecting a clear zone formation on CMC-congo Red Agar. There were 20 isolates of lactic acid bacteria from fecal pellets, coelom fluid, and gastrointestinal tract of nypa worm. Ten isolates (50%) had cellulolytic activity on CMC-phenol red agar. Eight isolates were closely related to the genus Lactobacillus, while two isolates were closely related to the genus Bacillus. These cellulolytic bacteria could be developed further as probiotic in Nypa worm feed.

scholarly journals Isolation of Bifidobacterium from Infant’s Feces and Its Antimicrobial Activity

2020 ◽  
Vol 2 ◽  
pp. 00002
Author(s):  
Dyah Fitri Kusharyati ◽  
Pancrasia Maria Hendrati ◽  
Dini Ryandini ◽  
Tsani Abu Manshur ◽  
Meilany Ariati Dewi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Inhibition Activity ◽  
Antimicrobial Substance ◽  
Inhibitory Zone ◽  
E Coli

<p class="Abstract"><i>Bifidobacterium</i> is a group of Lactic Acid Bacteria (LAB) that commonly found in the gastrointestinal tract and vagina. LAB has many health benefits, such as produce an antimicrobial substance against a pathogen. This research aims to isolate <i>Bifidobacterium</i> from an infant’s feces and know its antimicrobial activity against <i>Escherichia coli</i> and <i>Candida albicans.</i> A total of 5 isolates <i>Bifidobacterium</i> spp. were isolated from the sample. <span lang="EN">The largest inhibitory activity against <i>E. coli</i> was shown by isolate Bb3F, with the inhibitory zone of 10.80 mm. While the largest inhibition activity against <i>C. albicans</i> was shown by isolate Bb1B and Bb3F with the inhibitory zone of 9.70 mm.</span><o:p></o:p></p>

Emergence of Probiotics in Therapeutic Applications

2009 ◽  
Vol 2 (1) ◽  
pp. 383-389
Author(s):  
Barun K Bhattacharyya
Keyword(s):  
Health Care ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Functional Foods ◽  
Saccharomyces Boulardii ◽  
Lactobacillus Species ◽  
Therapeutic Applications ◽  
Probiotic Strains ◽  
Good Number

The gastrointestinal tract of human body is considered as an ecosystem which had been developed physiologically and microbiologically. The probiotic organisms (lactic acid bacteria) are one of the most important groups present in the gastrointestinal tract. A good number of Lactobacillus species, Bifidobacterium species, Saccharomyces boulardii and some other microbes are now used as probiotic strains. These probiotic organisms are marketed as health care products, functional foods or food supplements. The details of clinical, therapeutic applications and safety aspects of probiotics are discussed.

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