scholarly journals Plant Defense Enzymes Activated in Bean Plants by Aqueous Extract from Pycnoporus sanguineus Fruiting Body

Plant Science ◽  
10.5772/50686 ◽  
2012 ◽  
Author(s):  
Jose Renato ◽  
Clair Aparecida ◽  
Odair Jose ◽  
Katia Regina Freitas Schwan-Estrada ◽  
Lindomar Assi ◽  
...  
2020 ◽  
Vol 19 (3) ◽  
pp. 302-306
Author(s):  
Guilherme Valcorte ◽  
Elio José Santini ◽  
Maiara Talgatti ◽  
Laura Hoffmann de Oliveira ◽  
Amanda Grassmann da Silveira

The search for natural wood preservatives is becoming increasingly evident due to industrial preservatives presenting several environmental restrictions, such as soil, water and ecosystems contamination. Therefore, the objective of the present study is to evaluate the antifungal activity of Cinnamomum zeylanicum bark natural extract subjected to rotting fungi. In order to obtain the aqueous extract, two methods were performed with different concentrations, 50 and 100 g L-1, using Pycnoporus sanguineus e Gloeophyllum trabeum, mycelium. In the first, we kept the C. Zeylanicum bark with distilled water for 1 h in water bath and, in the second, the same concentrations were used, however, this was kept in a recipient for 24 h. Then, the extract was subjected to sterilization along with the BDA medium in autoclave at 120ºC for 20 min, they were then put in petri dishes for later evaluation of the mycelium root growth compared with the control. The fungi Pycnoporus sanguineus presented higher fungal activity, obtaining total inhibition of all treatments and extract concentrations, however, for the fungi Gloeophyllum trabeum, the 100 g L-1 concentration warmed in water bath obtained a better result than the others. Therefore, it can be concluded that the Cinnamomum zeylanicum aqueous extract presented inhibitory potential. Considering this, we suggest that more studies with this thematic should be carried out, seeking to discover new alternatives for wood preservatives that are less damaging to the environment and to mankind.


2018 ◽  
Vol 44 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Gilmar Franzener ◽  
Kátia Regina Freitas Schwan-Estrada ◽  
Gabriela Silva Moura ◽  
Odair José Kuhn ◽  
José Renato Stangarlin

ABSTRACT Secondary compounds of medicinal plants can activate defense mechanisms in plants against pathogens. The aqueous extract (AE) of Corymbia citriodora has shown that activity, but there is scarce information about the involved mechanisms of action. Therefore, this study aimed to evaluate the effect of AE on the induction of defense enzymes and protection of cucumber from Colletotrichum lagenarium. Thus, the AE, autoclaved or not, was evaluated for its capability of protecting and inducing peroxidases, polyphenoloxidases, chitinases and β-1.3-glucanases, and phenylalanine ammonia-lyase. Distilled water and acibenzolar-S-methyl (50 mg a.i. L-1) were used as controls. The effect of AE at the concentrations of 1, 5, 10, 15 and 20% was also evaluated, as well as its local and systemic effect, and the effect of one or two applications. Pathogen inoculation or sample collection for determination of enzyme activity was performed at 72 hours after treatments. The AE reduced the severity of C. lagenarium and induced peroxidases and β-1.3-glucanases by 37.6, 67.2 and 122.7%, respectively. There was a reduction in the disease severity and an increase in peroxidases from the concentration of 5% AE. The latter showed only local effect and greater reduction in severity when two applications were performed. These results suggest the effect of AE in inducing resistance in cucumber and inducing peroxidases and β-1.3-glucanases.


Author(s):  
Ayami Nishimura ◽  
Anna Yoshioka ◽  
Keisuke Kariya ◽  
Naoki Ube ◽  
Kotomi Ueno ◽  
...  

ABSTRACT Plant defense responses are activated by various exogenous stimuli. We found that an aqueous extract of spent mushroom substrate used for the cultivation of Hypsizygus marmoreus induced defense responses in rice. Fractionation of the spent mushroom substrate extract indicated that the compounds responsible for this induction were neutral and hydrophilic molecules with molecular weights lower than 3 kDa. Compounds with these characteristics, namely glucose, fructose, and sucrose, were detected in the extract at concentrations of 17.4, 3.3, and 1.6 mM, respectively, and the treatment of rice leaves with these sugars induced defense responses. Furthermore, microarray analysis indicated that the genes involved in defense responses were commonly activated by the treatment of leaves with spent mushroom substrate extract and glucose. These findings indicate that the induction of defense responses by treatment with spent mushroom substrate extract is, at least in part, attributable to the sugar constituents of the extract.


2006 ◽  
Vol 52 (5) ◽  
pp. 363-368 ◽  
Author(s):  
Meeta Lavania ◽  
Puneet Singh Chauhan ◽  
S.V.S. Chauhan ◽  
Harikesh Bahadur Singh ◽  
Chandra Shekhar Nautiyal

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