scholarly journals Sensitive detection of cereal fractions that are toxic to celiac disease patients by using monoclonal antibodies to a main immunogenic gluten peptide

10.5772/47909 ◽  
2012 ◽  
Author(s):  
Carolina Sousa ◽  
Ana Real ◽  
M De Lourdes Moreno ◽  
Isabel Comino
Keyword(s):  
Monoclonal Antibodies ◽  
Celiac Disease ◽  
Sensitive Detection ◽  
Gluten Peptide

scholarly journals Sensitive detection of cereal fractions that are toxic to celiac disease patients by using monoclonal antibodies to a main immunogenic wheat peptide

2008 ◽  
Vol 87 (2) ◽  
pp. 405-414 ◽  
Author(s):  
Belén Morón ◽  
Ángel Cebolla ◽  
Hamid Manyani ◽  
Moisés Álvarez-Maqueda ◽  
Manuel Megías ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Celiac Disease ◽  
Sensitive Detection

scholarly journals Use of Monoclonal Antibodies in the Sensitive Detection and Neutralization of Botulinum Neurotoxin Serotype B

Toxins ◽  
2015 ◽  
Vol 7 (12) ◽  
pp. 5068-5078 ◽  
Author(s):  
Luisa Cheng ◽  
Thomas Henderson ◽  
Tina Lam ◽  
Larry Stanker
Keyword(s):  
Monoclonal Antibodies ◽  
Botulinum Neurotoxin ◽  
Sensitive Detection ◽  
Serotype B

scholarly journals Plasma Cells Are the Most Abundant Gluten Peptide MHC-expressing Cells in Inflamed Intestinal Tissues From Patients With Celiac Disease

2019 ◽  
Vol 156 (5) ◽  
pp. 1428-1439.e10 ◽  
Author(s):  
Lene Støkken Høydahl ◽  
Lisa Richter ◽  
Rahel Frick ◽  
Omri Snir ◽  
Kristin Støen Gunnarsen ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Plasma Cells ◽  
Gluten Peptide

Development of monoclonal antibodies for the sensitive detection and quantification of specific DNA lesions

1995 ◽  
Vol 121 (S1) ◽  
pp. A26-A26
Author(s):  
C. Lutze ◽  
K. -H. Glüsenkamp ◽  
J. Thomale ◽  
P. Nehls ◽  
M. F. Rajewsky
Keyword(s):  
Monoclonal Antibodies ◽  
Dna Lesions ◽  
Sensitive Detection ◽  
Detection And Quantification

scholarly journals Fine specificity of monoclonal antibodies against celiac disease–inducing peptides in the gluteome

2008 ◽  
Vol 88 (4) ◽  
pp. 1057-1066 ◽  
Author(s):  
Cristina Mitea ◽  
Yvonne Kooy-Winkelaar ◽  
Peter van Veelen ◽  
Arnoud de Ru ◽  
Jan W Drijfhout ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Celiac Disease ◽  
Fine Specificity

scholarly journals A200 HUMANIZED CELIAC-PRONE EPITHELIUM IN VITRO EXPRESS MHC-II AND CO-STIMULATORY MOLECULES NECESSARY FOR GLUTEN PEPTIDE PRESENTATION

2020 ◽  
Vol 3 (Supplement_1) ◽  
pp. 73-74
Author(s):  
S Rahmani ◽  
H J Galipeau ◽  
H Su ◽  
F G Chirdo ◽  
T F Didar ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Antigen Presentation ◽  
Mhc Class Ii ◽  
Inflammatory Responses ◽  
Mhc Ii ◽  
Gluten Peptide ◽  
Peptide Presentation ◽  
Stimulation Of

Abstract Background The role intestinal epithelial cells (IECs) play in the breakdown of tolerance to gluten at an early stage in celiac disease (CeD) is unclear. Epithelial stress is a feature of CeD, and although the triggers are largely unknown, it is accompanied by expression of several markers that could be involved in initiation of inflammatory responses. IECs have been shown to express MHC class II (MHC-II) molecules and participate in antigen presentation in several models. Whether IECs can participate in gluten peptide presentation, the major environmental trigger in celiac disease, is unknown. To study this, a model expressing human MHC-II, HLA DQ8 or HLA-DQ2, would be required. Aims To develop organoid monolayers from transgenic mice expressing human celiac risk genes: HLA-DQ8 and -DQ2. To investigate conditions leading to the induction of epithelial MHC-II and its main co-stimulatory molecules, CD80, CD86 and CD40, that could enable early gluten peptide presentation. Methods In order to show pathophysiological significance of the model, we used two approaches, either induction of inflammation in vivo through gluten sensitization, or direct stimulation of the monolayers using pro-inflammatory cytokines relevant in CeD, such as IFNγ. Mice were sensitized with Pepsin-Trypsin digested gliadin and cholera toxin (CT) once a week for 3 weeks, followed by a challenge phase in which they only received gliadin. Control mice received CT only. We then developed organoid monolayers from the duodenum followed by stimulation with 10 ng/ml IFNγ. Finally, markers necessary for gluten peptide presentation, the expression of MHC-II and its co-stimulatory molecules, were evaluated using flow cytometry. Results Both in vivo gluten sensitization and in vitro stimulation of the organoid derived monolayer with IFNγ induced a proinflammatory response, that independently primed the epithelium to express MHC-II molecules (p =0.02 and <0.0001, respectively). When in vivo sensitization and in vitro IFNγ stimulation were combined, epithelial MHC-II expression was further upregulated (p <0.0001). Lastly, only the combination of gluten sensitization and in vitro IFNγ induced expression of MHC-II co-stimulatory molecules, which are necessary for antigen presentation. Conclusions Our findings support that gluten induced-inflammation in vivo as well as independent stimuli that release IFNγ enhance the capacity of the IECs to express MHC-II molecules. However, co-stimulatory molecules are only expressed by the epithelium when both gluten tolerance is broken by in vivo sensitization and the organoid monolayers is further exposed to IFNγ. The results support the hypothesis that the epithelium participates in gluten peptide presentation and that this pathway is stimulated by both gluten-dependent and independent inflammation. Funding Agencies CIHRSupported by CIHR and a Farncombe Family Grant to EFV and TFD.

scholarly journals Cyclic and dimeric gluten peptide analogues inhibiting DQ2-mediated antigen presentation in celiac disease

2007 ◽  
Vol 15 (20) ◽  
pp. 6565-6573 ◽  
Author(s):  
Jiang Xia ◽  
Elin Bergseng ◽  
Burkhard Fleckenstein ◽  
Matthew Siegel ◽  
Chu-Young Kim ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Antigen Presentation ◽  
Gluten Peptide ◽  
Peptide Analogues
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