scholarly journals Porous Titanium by Powder Metallurgy for Biomedical Application: Characterization, Cell Citotoxity and in vivo Tests of Osseointegration

10.5772/47816 ◽  
2012 ◽  
Author(s):  
Luana Marotta Reis de Vasconcellos ◽  
Yasmin Rodarte ◽  
Renata Falchete do Prado ◽  
Luis Gustavo Oliveira de Vasconcellos ◽  
Mrio Lima de Alencastro Graa ◽  
...  
Keyword(s):  
Powder Metallurgy ◽  
Biomedical Application ◽  
Porous Titanium ◽  
In Vivo Tests

Antitumor Effect of Zwitterions of Imidazolium Derived from L-methionine in BALB/c Mice with Lymphoma L5178Y

2020 ◽  
Vol 17 (1) ◽  
pp. 33-39
Author(s):  
Karen C. Vargas-Castro ◽  
Ana M. Puebla Pérez ◽  
Irma I. Rangel-Salas ◽  
Jorge I. Delgado-Saucedo ◽  
José B. Pelayo-Vázquez ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Antitumor Activity ◽  
Antitumor Agent ◽  
Inhibitory Effect ◽  
Stabilizing Agent ◽  
Biological Compatibility ◽  
In Vivo Tests ◽  
Antitumoral Agent ◽  
Group 1

Background: In the therapy of cancer, several treatments have been designed using nanomaterials, among which gold nanoparticles (AuNPs) have been featured as a promising antitumoral agent. Our research group has developed the synthesis of gold nanoparticles L-AuNPs and D-AuNPs stabilized with zwitterions of imidazolium (L-1 and D-1) derived from L-methionine and D-methionine. Because the stabilizer agent is chiral, we observed through circular dichroism that AuNPs also present chirality; such chirality as well as the fact that the stabilizing agent contains fragments of methionine and imidazolium that are commonly involved in biological processes, opens up the possibility that this system may have biological compatibility. Additionally, the presence of methionine in the stabilizing agent opens the application of this system as a possible antitumor agent because methionine is involved in methylation processes of molecules such as DNA. Objective: The aim of this research is the evaluation of the antitumor activity of gold nanoparticles stabilized with zwitterions of imidazolium (L-AuNPs) derived from L-methionine in the model of BALB/c mice with lymphoma L5178Y. Methods: Taking as a parameter cell density, the evaluation of the inhibitory effect of L-AuNPs was carried out with a series of in vivo tests in BALB/c type mice; three groups of five mice each were formed (Groups 1, 2 and 3); all mice were i.p. inoculated with the lymphoblast murine L5178Y. Group 1 consisted of mice without treatment. In the Groups 2 and 3 the mice were treated with L-AuNPs at 0.3 mg/Kg on days 1, 7 and 14 by orally and intraperitonally respectively. Results: These results show low antitumor activity of these gold nanoparticles (L-NPsAu) but interestingly, the imidazolium stabilizing agent of gold nanoparticle (L-1) displayed promising antitumor activity. On the other hand, the enantiomer of L-1, (D-1) as well as asymmetric imidazole derivate from L-methionine (L-2), do not exhibit the same activity as L-1. Conclusion: The imidazolium stabilizing agent (L-1) displayed promising antitumor activity. Modifications in the structure of L-1 showed that, the stereochemistry (like D-1) and the presence of methionine fragments (like L-2) are determinants in the antitumor activity of this compound.

In Vitro Methods as a Tool in Neurotoxicity Studies

1995 ◽  
Vol 23 (4) ◽  
pp. 491-496
Author(s):  
Hanna Tähti ◽  
Leila Vaalavirta ◽  
Tarja Toimela
Keyword(s):  
Risk Evaluation ◽  
Toxicity Testing ◽  
In Vitro Models ◽  
In Vitro Tests ◽  
Industrial Chemicals ◽  
Mercury Compounds ◽  
Toxicological Data ◽  
In Vivo Tests

— There are several hundred industrial chemicals with neurotoxic potential. The neurotoxic risks of most of these chemicals are unknown. Additional methods are needed to assess the risks more effectively and to elucidate the mechanisms of neurotoxicity more accurately than is possible with the conventional methods. This paper deals with general tasks concerning the use of in vitro models in the evaluation of neurotoxic risks. It is based on our previous studies with various in vitro models and on recent literature. The induction of glial fibrillary acidic protein in astrocyte cultures after treatment with known neurotoxicants (mercury compounds and aluminium) is discussed in more detail as an important response which can be detected in vitro. When used appropriately with in vivo tests and with previous toxicological data, in vitro neurotoxicity testing considerably improves risk assessment. The incorporation of in vitro tests into the early stages of risk evaluation can reduce the number of animals used in routine toxicity testing, by identifying chemicals with high neurotoxic potential.

scholarly journals Role of in vitro and in vivo tests of hypersensitivity in beryllium workers.

1977 ◽  
Vol 30 (1) ◽  
pp. 24-28 ◽  
Author(s):  
C D Price ◽  
W J Williams ◽  
A Pugh ◽  
D H Joynson
Keyword(s):  
In Vivo Tests

Evaluation of immunomodulatory activity of two potential probiotic Lactobacillus strains by in vivo tests

Anaerobe ◽  
2015 ◽  
Vol 35 ◽  
pp. 22-27 ◽  
Author(s):  
Dayong Ren ◽  
Chang Li ◽  
Yanqing Qin ◽  
Ronglan Yin ◽  
Shouwen Du ◽  
...  
Keyword(s):  
Immunomodulatory Activity ◽  
Probiotic Lactobacillus ◽  
In Vivo Tests

Osteoconductivity of Superhydrophilic Ti- and Zr-Alloy for Biomedical Application

2016 ◽  
Vol 879 ◽  
pp. 2524-2527
Author(s):  
Masazumi Okido ◽  
Kensuke Kuroda
Keyword(s):  
Contact Angle ◽  
Water Contact Angle ◽  
Strong Influence ◽  
Biomedical Application ◽  
Hydrophobic Surface ◽  
Hydrophilic Surface ◽  
Water Contact ◽  
Zr Alloy ◽  
Zr Alloys

Surface hydrophilicity is considered to have a strong influence on the biological reactions of bone-substituting materials. However, the influence of a hydrophilic or hydrophobic surface on the osteoconductivity is not completely clear. In this study, we produced super-hydrophilic and hydrophobic surface on Ti-and Zr-alloys. Hydrothermal treatment at 180 oC for 180 min. in the distilled water and immersion in x5 PBS(-) brought the super-hydrophilic surface (water contact angle < 10 (deg.)) and heat treatment of as-hydrothermaled the hydrophobic surface. The osteoconductivity of the surface treated samples with several water contact angle was evaluated by in vivo testing. The surface properties, especially water contact angle, strongly affected the osteoconductivity and protein adsorbability, and not the surface substance.

Mechanical Performance and In Vivo Tests of an Acrylic Bone Cement Filled with Bioactive Sepia Officinalis Cuttlebone

2010 ◽  
Vol 21 (1) ◽  
pp. 113-125 ◽  
Author(s):  
S. García-Enriquez ◽  
H. E. R. Guadarrama ◽  
I. Reyes-González ◽  
E. Mendizábal ◽  
C. F. Jasso-Gastinel ◽  
...  
Keyword(s):  
Bone Cement ◽  
Mechanical Performance ◽  
Sepia Officinalis ◽  
Acrylic Bone Cement ◽  
In Vivo Tests

Report of the IWGT working group on strategies and interpretation of regulatory in vivo tests

2007 ◽  
Vol 627 (1) ◽  
pp. 78-91 ◽  
Author(s):  
D.J. Tweats ◽  
D. Blakey ◽  
R.H. Heflich ◽  
A. Jacobs ◽  
S.D. Jacobsen ◽  
...  
Keyword(s):  
Working Group ◽  
In Vivo Tests

A brief overview on role of graphene based material in therapeutic management of inflammatory response signalling cascades

2020 ◽  
Vol 21 ◽  
pp. 25-36
Author(s):  
Ruchira Das ◽  
◽  
Priyanka Sow ◽  
Sudatta Dey ◽  
Asmita Samadder ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Water Solubility ◽  
Biomedical Application ◽  
Rapid Development ◽  
Graphene Quantum Dots ◽  
Biological Response ◽  
Vital Role ◽  
Therapeutic Management ◽  
In Vivo Models

Graphene is a novel, sp2 carbon atoms bonded, two-dimensional nano-material. Due to their favorable electronic, thermal, optical, and mechanical property, graphene and its derivatives, like graphene oxide (GO) and graphene quantum dots (GQDs) are used in widespread applications. The outstanding potentials of these compounds in the field of nanoelectronics, composite materials, sensors, energy technology etc helped in the rapid development in their functionalization, modulatory effects on various systems of our body. GQDs has been suggested as a new nanomaterial with improved biocompatibility, biodegradability, water solubility and considerably low cytotoxic effects in in vivo models, and are applicable for altering immune responses based on quantum confinement and edge effect properties. The review particularly elucidates the mechanistic approach by which graphene and/ or its derivatives and/ or their nano-compound aid in therapeutic management against myriads of immunological perspectives. GQDs have unique physiochemical properties with carbon sheets showcases out-standing biological response against immunological interventions by altering the activities of t-cell lymphocytes. On the contrary GO plays a vital role in eliciting inflammatory signaling factors by controlling proinflammation and an anti-inflammatory response. Therefore, this review shall help the readers to have an overview of the biomedical application of graphene and its derivatives to design target specific drugs to regulate the immune response based prognosis andcure.

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