scholarly journals Endosomal Escape Pathways for Non-Viral Nucleic Acid Delivery Systems

10.5772/46006 ◽  
2012 ◽  
Author(s):  
Wanling Liang ◽  
Jenny K. W. Lam
Keyword(s):  
Nucleic Acid ◽  
Delivery Systems ◽  
Endosomal Escape ◽  
Nucleic Acid Delivery ◽  
Viral Nucleic Acid

scholarly journals Viral Mimicry as a Design Template for Nucleic Acid Nanocarriers

2021 ◽  
Vol 9 ◽  
Author(s):  
Ina F. de la Fuente ◽  
Shraddha S. Sawant ◽  
Mark Q. Tolentino ◽  
Patrick M. Corrigan ◽  
Jessica L. Rouge
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Molecular Mechanisms ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Endosomal Escape ◽  
Nucleic Acid Delivery ◽  
Metastable Structure ◽  
Oligonucleotide Delivery ◽  
Genome Protection

Therapeutic nucleic acids hold immense potential in combating undruggable, gene-based diseases owing to their high programmability and relative ease of synthesis. While the delivery of this class of therapeutics has successfully entered the clinical setting, extrahepatic targeting, endosomal escape efficiency, and subcellular localization. On the other hand, viruses serve as natural carriers of nucleic acids and have acquired a plethora of structures and mechanisms that confer remarkable transfection efficiency. Thus, understanding the structure and mechanism of viruses can guide the design of synthetic nucleic acid vectors. This review revisits relevant structural and mechanistic features of viruses as design considerations for efficient nucleic acid delivery systems. This article explores how viral ligand display and a metastable structure are central to the molecular mechanisms of attachment, entry, and viral genome release. For comparison, accounted for are details on the design and intracellular fate of existing nucleic acid carriers and nanostructures that share similar and essential features to viruses. The review, thus, highlights unifying themes of viruses and nucleic acid delivery systems such as genome protection, target specificity, and controlled release. Sophisticated viral mechanisms that are yet to be exploited in oligonucleotide delivery are also identified as they could further the development of next-generation nonviral nucleic acid vectors.

Non-Viral Nucleic Acid Delivery: Key Challenges and Future Directions

2011 ◽  
Vol 8 (3) ◽  
pp. 235-244 ◽  
Author(s):  
Mahmoud Elsabahy ◽  
Adil Nazarali ◽  
Marianna Foldvari
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Delivery ◽  
Viral Nucleic Acid ◽  
Future Directions

Reduction-Responsive Nucleic Acid Delivery Systems To Prevent In-Stent Restenosis in Rabbits

2019 ◽  
Vol 11 (31) ◽  
pp. 28307-28316 ◽  
Author(s):  
Weijie Ye ◽  
Yiming Chen ◽  
Wenxiong Tang ◽  
Na Zhang ◽  
Zhonghao Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Delivery Systems ◽  
Nucleic Acid Delivery ◽  
Stent Restenosis ◽  
In Stent Restenosis

scholarly journals The Impact of Alkyl‐Chain Purity on Lipid‐Based Nucleic Acid Delivery Systems – Is the Utilization of Lipid Components with Technical Grade Justified?

ChemPhysChem ◽  
2019 ◽  
Vol 20 (16) ◽  
pp. 2110-2121
Author(s):  
Dorota Pawlowska ◽  
Christopher Janich ◽  
Andreas Langner ◽  
Bodo Dobner ◽  
Christian Wölk ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Alkyl Chain ◽  
Delivery Systems ◽  
Nucleic Acid Delivery ◽  
Technical Grade ◽  
The Impact ◽  
Lipid Components

scholarly journals Peptide-Assisted Nucleic Acid Delivery Systems on the Rise

2021 ◽  
Vol 22 (16) ◽  
pp. 9092
Author(s):  
Shabnam Tarvirdipour ◽  
Michal Skowicki ◽  
Cora-Ann Schoenenberger ◽  
Cornelia G. Palivan
Keyword(s):  
Nucleic Acid ◽  
Side Effects ◽  
Nucleic Acids ◽  
Delivery Systems ◽  
Inorganic Nanoparticles ◽  
Water Soluble ◽  
Nucleic Acid Delivery ◽  
Subcellular Organelles ◽  
Cell Internalization ◽  
Site Of Action

Concerns associated with nanocarriers’ therapeutic efficacy and side effects have led to the development of strategies to advance them into targeted and responsive delivery systems. Owing to their bioactivity and biocompatibility, peptides play a key role in these strategies and, thus, have been extensively studied in nanomedicine. Peptide-based nanocarriers, in particular, have burgeoned with advances in purely peptidic structures and in combinations of peptides, both native and modified, with polymers, lipids, and inorganic nanoparticles. In this review, we summarize advances on peptides promoting gene delivery systems. The efficacy of nucleic acid therapies largely depends on cell internalization and the delivery to subcellular organelles. Hence, the review focuses on nanocarriers where peptides are pivotal in ferrying nucleic acids to their site of action, with a special emphasis on peptides that assist anionic, water-soluble nucleic acids in crossing the membrane barriers they encounter on their way to efficient function. In a second part, we address how peptides advance nanoassembly delivery tools, such that they navigate delivery barriers and release their nucleic acid cargo at specific sites in a controlled fashion.

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