scholarly journals Mechanisms of Hepatocellular Dysfunction and Regeneration: Enzyme Inhibition by Nitroimidazole and Human Liver Regeneration

10.5772/39114 ◽  
2012 ◽  
Author(s):  
Rakesh Sharma
Keyword(s):  
Liver Regeneration ◽  
Enzyme Inhibition ◽  
Human Liver

scholarly journals Amnion-Derived Mesenchymal Stromal/Stem Cell Paracrine Signals Potentiate Human Liver Organoid Differentiation: Translational Implications for Liver Regeneration

2021 ◽  
Vol 8 ◽  
Author(s):  
Antonio Lo Nigro ◽  
Alessia Gallo ◽  
Matteo Bulati ◽  
Giampiero Vitale ◽  
Diego Sebastian Paini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Liver Regeneration ◽  
Conditioned Medium ◽  
Liver Diseases ◽  
Human Liver ◽  
Progenitor Cell ◽  
Liver Repair ◽  
Liver Organoids ◽  
End Stage

The prevalence of end-stage liver diseases has reached very high levels globally. The election treatment for affected patients is orthotopic liver transplantation, which is a very complex procedure, and due to the limited number of suitable organ donors, considerable research is being done on alternative therapeutic options. For instance, the use of cell therapy, such as the transplantation of hepatocytes to promote liver repair/regeneration, has been explored, but standardized protocols to produce suitable human hepatocytes are still limited. On the other hand, liver progenitor and multipotent stem cells offer potential cell sources that could be used clinically. Different studies have reported regarding the therapeutic effects of transplanted mesenchymal stromal/stem cells (MSCs) on end-stage liver diseases. Moreover, it has been shown that delivery of MSC-derived conditioned medium (MSC-CM) can reduce cell death and enhance liver proliferation in fulminant hepatic failure. Therefore, it is believed that MSC-CM contains many factors that probably support liver regeneration. In our work, we used an in vitro model of human liver organoids to study if the paracrine components secreted by human amnion-derived MSCs (hAMSCs) affected liver stem/progenitor cell differentiation. In particular, we differentiated liver organoids derived from bipotent EpCAM+ human liver cells and tested the effects of hAMSC secretome, derived from both two-dimensional (2D) and three-dimensional (3D) hAMSC cultures, on that model. Our analysis showed that conditioned medium (CM) produced by 3D hAMSCs was able to induce an over-expression of mature hepatocyte markers, such as ALB, NTCP, and CYP3A4, compared with both 2D hAMSC cultures and the conventional differentiation medium (DM). These data were confirmed by the over-production of ALB protein and over-activity of CYP3A4 observed in organoids grown in 3D hAMSC-CM. Liver repair dysfunction plays a role in the development of liver diseases, and effective repair likely requires the normal functioning of liver stem/progenitor cells. Herein, we showed that hAMSC-CM produced mainly by 3D cultures had the potential to increase hepatic stem/progenitor cell differentiation, demonstrating that soluble factors secreted by those cells are potentially responsible for the reaction. This work shows a potential approach to improve liver repair/regeneration also in a transplantation setting.

Expression of augmenter of liver regeneration (ALR) in human liver cirrhosis and carcinoma

2005 ◽  
Vol 47 (1) ◽  
pp. 57-66 ◽  
Author(s):  
W E Thasler ◽  
T Schlott ◽  
P Thelen ◽  
C Hellerbrand ◽  
F Bataille ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Liver Regeneration ◽  
Human Liver ◽  
Augmenter Of Liver Regeneration ◽  
Human Liver Cirrhosis

scholarly journals Erratum to “Human liver regeneration in advanced cirrhosis is organized by the portal tree” [J Hepatol 66 (2017) 778–786]

2017 ◽  
Vol 67 (2) ◽  
pp. 430-436
Author(s):  
Katalin Dezső ◽  
András Rókusz ◽  
Edina Bugyik ◽  
Armanda Szücs ◽  
András Szuák ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Human Liver ◽  
Advanced Cirrhosis

scholarly journals Adult-Derived Human Liver Stem/Progenitor Cells Infused 3 Days Postsurgery Improve Liver Regeneration in a Mouse Model of Extended Hepatectomy

2017 ◽  
Vol 26 (2) ◽  
pp. 351-364 ◽  
Author(s):  
Astrid Herrero ◽  
Julie Prigent ◽  
Catherine Lombard ◽  
Valérie Rosseels ◽  
Martine Daujat-Chavanieu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Cell Therapy ◽  
Liver Regeneration ◽  
Progenitor Cells ◽  
Human Liver ◽  
Liver Weight ◽  
Vehicle Group ◽  
Hepatic Tissue ◽  
Extended Hepatectomy ◽  
Extensive Hepatectomy

There is growing evidence that cell therapy constitutes a promising strategy for liver regenerative medicine. In the setting of hepatic cancer treatments, cell therapy could prove a useful therapeutic approach for managing the acute liver failure that occurs following extended hepatectomy. In this study, we examined the influence of delivering adult-derived human liver stem/progenitor cells (ADHLSCs) at two different early time points in an immunodeficient mouse model ( Rag2−/-IL2Rg -/-) that had undergone a 70% hepatectomy procedure. The hepatic mesenchymal cells were intrasplenically infused either immediately after surgery ( n = 26) or following a critical 3-day period ( n = 26). We evaluated the cells' capacity to engraft at day 1 and day 7 following transplantation by means of human Alu qPCR quantification, along with histological assessment of human albumin and α-smooth muscle actin. In addition, cell proliferation (anti-mouse and human Ki-67 staining) and murine liver weight were measured in order to evaluate liver regeneration. At day 1 posttransplantation, the ratio of human to mouse cells was similar in both groups, whereas 1 week posttransplantation this ratio was significantly improved ( p < 0.016) in mice receiving ADHLSC injection at day 3 posthepatectomy (1.7%), compared to those injected at the time of surgery (1%). On the basis of liver weight, mouse liver regeneration was more extensive 1 week posttransplantation in mice transplanted with ADHLSCs (+65.3%) compared to that of mice from the sham vehicle group (+42.7%). In conclusion, infusing ADHLSCs 3 days after extensive hepatectomy improves the cell engraftment and murine hepatic tissue regeneration, thereby confirming that ADHLSCs could be a promising cell source for liver cell therapy and hepatic tissue repair.

Glucuronidation of 3′-azido-3′-deoxythymidine in human liver microsomes: enzyme inhibition by drugs and steroid hormones

1992 ◽  
Vol 1139 (1-2) ◽  
pp. 20-24 ◽  
Author(s):  
Regine Herber ◽  
Jacques Magdalou ◽  
Marc Haumont ◽  
Roselyne Bidault ◽  
Helmuth van Es ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Enzyme Inhibition ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes

scholarly journals Human Liver Regeneration After Major Hepatectomy

1991 ◽  
Vol 213 (3) ◽  
pp. 227-229 ◽  
Author(s):  
MIIN-FU CHEN ◽  
TSANN-LONG HWANG ◽  
CHIEN-FU HUNG
Keyword(s):  
Liver Regeneration ◽  
Human Liver ◽  
Major Hepatectomy
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