scholarly journals Methods for Sperm Selection for In Vitro Fertilization

10.5772/39049 ◽  
2012 ◽  
Author(s):  
Nicols M. ◽  
Pablo Bosch
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Selection ◽  
Vitro Fertilization ◽  
Selection For

scholarly journals Methods of Sperm Selection for In-Vitro Fertilization

2021 ◽  
Author(s):  
Abimibola Nanna
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Cell ◽  
Sperm Concentration ◽  
Cell Selection ◽  
Sperm Selection ◽  
Vitro Fertilization ◽  
Sperm Dna Damage ◽  
Microfluidic Separation ◽  
Infertile Men

50–60% of infertility cases are as a result of male infertility and infertile men semen sample is characterize with poor motility, abnormal morphology, low sperm concentration, azoospermic and increased levels of sperm DNA damage. As a result of this heterogeneity of the ejaculate, sperm selection has become a necessary step to carry out prior to in vitro fertilization. Furthermore, the choice of sperm cell selection techniques depend on sperm concentration and sperm biology and the recovery of highly functional sperm cell population depend on the combination of more than one technique in some cases. The regular sperm cell selection methods in ART laboratory are swim up, density gradient, simple wash and other advanced and emerging sperm selection techniques which include hyaluronic acid mediated sperm binding, Zeta potential, hypoosmotic swelling test, magnetic activated cell sorting and microfluidic separation of sperm cells. The various methods have its own advantages and disadvantages which may be applicable to the individual need of infertile men and its effect on ART outcome.

scholarly journals EFFECT OF THE USE OF TWO SPERM SELECTION TECHNIQUES FOR IN VITRO PRODUCTION OF ALPACA EMBRYOS

SPERMOVA ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 67-72
Author(s):  
Mijail Contreras Huamani ◽  
◽  
Mary Naveros ◽  
Cesar Olaguivel
Keyword(s):  
In Vitro Fertilization ◽  
Cumulus Cells ◽  
Sperm Selection ◽  
Percoll Gradient ◽  
In Vitro Production ◽  
Vitro Fertilization ◽  
Maturation Medium ◽  
Blastocyst Rate ◽  
Vitro Production

The objective of this research was to evaluate the effect of the use of two sperm selection techniques for in vitro production of alpaca embryos. The ovaries and testis were collected from the local slaughterhouse and transport to 37 ° C in saline solution (0.9%) supplemented with gentamicin. Quality I, II and II oocytes were incubated in a maturation medium for 32 h at 38.5 ° C and 5% O2 and 5% CO2. For in vitro fertilization, sperm from the epididymis were selected using the Percoll gradient and Swim up technique. 18h after the oocytes were incubated with the sperm, these were denuded from the cumulus cells and cultured in SOFaa culture medium for 7 days. Morula and blastocyst rate and their morphological quality are evaluated at day 7 of culture. From a total of 370 ovaries, 1,137 oocytes were recovered, making an average of 3.6 oocytes / ovary. After the maturation and fertilization process and in vitro culture, the blastocyst rate was 8.43 ± 6.04% and 3.89 ± 1.75%, for oocytes fertilized with sperm selected with Percoll gradient and Swim up, respectively, not finding significant statistical differences (p> 0.05), between the groups. In conclusion, the in vitro fertilization of alpaca oocytes with spermatozoa selected with two selection techniques (percoll and swim up) did not significantly influence the quantity and quality of morulae and blastocysts at day 7 of embryo culture.

scholarly journals Is it ever morally permissible to select for deafness in one’s child?

2019 ◽  
Vol 23 (1) ◽  
pp. 3-15 ◽  
Author(s):  
Jacqueline Mae Wallis
Keyword(s):  
In Vitro Fertilization ◽  
Cultural Identity ◽  
Deaf Child ◽  
Genetic Diagnosis ◽  
Neutral Model ◽  
Vitro Fertilization ◽  
Genetic Deafness ◽  
Genetic Technologies ◽  
Selection For

Abstract As reproductive genetic technologies advance, families have more options to choose what sort of child they want to have. Using preimplantation genetic diagnosis (PGD), for example, allows parents to evaluate several existing embryos before selecting which to implant via in vitro fertilization (IVF). One of the traits PGD can identify is genetic deafness, and hearing embryos are now preferentially selected around the globe using this method. Importantly, some Deaf families desire a deaf child, and PGD–IVF is also an option for them. Selection for genetic deafness, however, encounters widespread disapproval in the hearing community, including mainstream philosophy and bioethics. In this paper I apply Elizabeth Barnes’ value-neutral model of disability as mere-difference to the case of selecting for deafness. I draw on evidence from Deaf Studies and Disability Studies to build an understanding of deafness, the Deaf community, and the circumstances relevant to reproductive choices that may obtain for some Deaf families. Selection for deafness, with deafness understood as mere-difference and valued for its cultural identity, need not necessitate impermissible moral harms. I thus advocate that it is sometimes morally permissible to select for deafness in one’s child.

P002: Soluble CD146, an innovat ive and non-invasive biomarker of embryo selection for in-vitro fertilization

2019 ◽  
Vol 175 ◽  
pp. S7
Author(s):  
S. Bouvier ◽  
O. Paulmyer-Lacroix ◽  
E. Kaspi ◽  
A. Bertaud ◽  
A. Leroyer ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Selection ◽  
Vitro Fertilization ◽  
Non Invasive ◽  
Selection For ◽  
Soluble Cd146

scholarly journals Soluble CD146, an innovative and non-invasive biomarker of embryo selection for in vitro fertilization

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173724 ◽  
Author(s):  
Sylvie Bouvier ◽  
Odile Paulmyer-Lacroix ◽  
Nicolas Molinari ◽  
Alexandrine Bertaud ◽  
Marine Paci ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Selection ◽  
Vitro Fertilization ◽  
Non Invasive ◽  
Selection For ◽  
Soluble Cd146
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