scholarly journals Two-Dimensional Gel Electrophoresis Reveals Differential Protein Expression Between Individual Daphnia

10.5772/38847 ◽  
2012 ◽  
Author(s):  
Darren J. ◽  
Gary B. ◽  
W. Kelley
Keyword(s):  
Protein Expression ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Differential Protein Expression ◽  
Two Dimensional Gel Electrophoresis ◽  
Differential Protein

scholarly journals Comparison ofHelicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-16 ◽  
Author(s):  
Arinze S. Okoli ◽  
Mark J. Raftery ◽  
George L. Mendz
Keyword(s):  
Protein Expression ◽  
Gel Electrophoresis ◽  
Developed Countries ◽  
Protein Isoforms ◽  
Two Dimensional ◽  
Differential Protein Expression ◽  
Two Dimensional Gel Electrophoresis ◽  
Differential Protein ◽  
Huh7 Cells ◽  
The Developed Countries

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. Hepatitis B or C infections are the main causes of HCC with hepatitis C being the major risk factor for liver cancer in the developed countries. Recently, complications with bacteria of the genusHelicobacterhave been associated with HCV-induced HCC. To further understand the mechanisms leading to the development of HCC in the presence of HCV and/orHelicobacterspp., investigation of the differential protein expression in Huh7 cells harbouring HCV-replicon, and replicon cured-Huh7 cells cocultured withH. biliswas done employing two-dimensional gel electrophoresis and mass spectrometry. In the transfected-Huh7 cells exposed to sublethal inoculum densities ofH. bilis, 53 different proteins were identified comprising of 28 upregulated and 16 downregulated proteins including 9 potential protein isoforms; in the cured Huh7 cells, 45 different proteins were identified including 33 upregulated, 8 downregulated and, 9 potential protein isoforms.H. bilisaffected the modulation of proteins involved in different pathways of Huh7-derived cells physiology including proteins involved in the progression from dysplasia to neoplasm. The result also indicated that the response of the Huh7-derived cells to the presence ofH. bilisdepended on whether or not HCV replicon was present.

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