scholarly journals What Flow Cytometry can Tell Us About Marine Micro-Organisms – Current Status and Future Applications

10.5772/38616 ◽  
2012 ◽  
Author(s):  
A. Manti ◽  
S. Papa ◽  
P. Boi
Keyword(s):  
Flow Cytometry ◽  
Current Status ◽  
Micro Organisms

Rapid detection of microbial contamination in grape juice by flow cytometry

OENO One ◽  
1999 ◽  
Vol 33 (1) ◽  
pp. 31 ◽  
Author(s):  
Marielle Bouix ◽  
Agnès Grabowski ◽  
Monique Charpentier ◽  
Jean-Yves Leveau ◽  
Bruno Duteurtre
Keyword(s):  
Flow Cytometry ◽  
Reference Method ◽  
Grape Juice ◽  
Plate Count ◽  
Contamination Level ◽  
Cell Counts ◽  
Direct Cell ◽  
Micro Organisms ◽  
High Degree

<p style="text-align: justify;">This study presents an application of flow cytometry to evaluate rapidly the viable micro-organisms in grape juice. In this method, viable cells are firstly specitically labelled with a fluorescent reagent. The sample is then injected into the flow cytometer where the labelled micro-organisms are individually illuminated by a laser beam. The emission of fluorescence is measured. The system counts the number of fluorescent events and prints out a histogram of the fluorescence intensity which is characteristic of the micro-organism being analysed. In laboratory conditions, preliminary trials have been undertaken with an artificially inoculated grape juice with pure yeast and bacteria cultures. This method succeeded in counting simultaneously yeasts and bacteria within 15 minutes, with a high degree of sensitivity, 5.10<sup>3</sup> yeasts perml and 5.10<sup>4</sup> bacteria per ml. This technique can also be applied to the detection of mould contamination and the test has been done with <em>Botrytis</em> spores. The method makes direct cell counts possible and is capable of analysing 30 samples per hour. It can be automatised and easily used in industrial laboratory. During the last harvest, more than a thousand of must samples were controled using this technique. The results let to determine the yeast contamination level of a grape juice tank even before unloading. The results obtained by flow cytometry were compared to the plate count reference method. The correlation between cytometry and count by plate culture was 99 p. cent for the threshold of 5.1 0<sup>4</sup> yeasts/ml which seemed to point out a high contamination. By using this flow cytometry method during the harvest period, the results were supplied in real time. This allowed a rapid selection of the musts, depending upon the scale of their contamination and improved the quality of the wine by corrective actions.</p>

Semen Sexing in Bovine: Current Status and the Need to Develop Alternative Techniques

2021 ◽  
Vol 1 (1) ◽  
pp. 17-31
Author(s):  
Himani Joshi ◽  
Manya Mathur ◽  
AK Mohanty ◽  
Sudarshan Kumar ◽  
JK Kaushik ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Review Paper ◽  
Current Status ◽  
Sperm Cells ◽  
Non Invasive ◽  
Bovine Sperm ◽  
The World ◽  
Alternative Techniques ◽  
Sperm Sorting ◽  
Cost Efficient

Livestock industry is one of the important pillars for country economy. Sperm sexing has the potential of significantly improving the quality and quantity of production in the livestock industries. Over years several attempts, involving several different parameters of sperm cells, have been made to develop an efficient sperm sorting procedure. Till date, the only commercially available method for bovine sperm is flow cytometry. The disadvantages associated with this approach, however, points towards the need for developing a more non-invasive and cost efficient approach for separation of X and Y chromosome bearing spermatozoa. In this review paper, we attempt to highlight all the previously developed approaches, as well as other emerging immunological and proteomics based methods that might have the potential of being developed as a promising breakthrough approach for semen sexing. Furthermore, we discuss the advantages and applications of semen sexing in India and around the world.

Current status of CD34+ cell analysis by flow cytometry: The ISHAGE guidelines

1997 ◽  
Vol 17 (2-3) ◽  
pp. 21-29 ◽  
Author(s):  
Ian Chin-Yee ◽  
Michael Keeney ◽  
Lori Anderson ◽  
Rakesh Nayar ◽  
D. Robert Sutherland
Keyword(s):  
Flow Cytometry ◽  
Current Status ◽  
Cell Analysis

scholarly journals Current status of sexing mammalian spermatozoa

Reproduction ◽  
2002 ◽  
pp. 733-743 ◽  
Author(s):  
◽  
DL Garner
Keyword(s):  
Flow Cytometry ◽  
X Chromosome ◽  
Artificial Insemination ◽  
Cell Sorting ◽  
Genetic Diseases ◽  
Current Status ◽  
Optimal Conditions ◽  
Theoretical Limit ◽  
In Series ◽  
Mammalian Spermatozoa

Thousands of offspring have now been produced via artificial insemination with spermatozoa sexed by flow cytometry and cell sorting. We are unaware of any other practical approach to sexing spermatozoa that maintains fertility. Accuracy of sexing usually is 85-95% in most species, but somewhat lower with human spermatozoa. Spermatozoa are sexed in series, one at a time, at routine rates of about 3000 live spermatozoa of each sex per second for most species, and nearly twice that rate under optimal conditions for some species. Owing to various constraints and statistical considerations, there appears to be an upper theoretical limit to sexing spermatozoa of about 10,000 live spermatozoa of each sex per second with current methodology. About a quarter of the spermatozoa processed are sexed; the rest are discarded in the process or lost due to logistical constraints. Spermatozoa undergo some damage during sorting, although much less in terms of viability than with routine cryopreservation; fertility is lower with sexed than control spermatozoa. Offspring from sexed spermatozoa appear to have no more abnormalities than do controls, and both groups grow and thrive similarly. Despite high costs and complex procedures, sexing spermatozoa, usually followed by cryopreservation, is being used commercially for cattle and horse production in several countries, and is used to produce girls to avoid X-chromosome-linked genetic diseases.

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