scholarly journals Flow Cytometry Analysis of Intracellular Protein

10.5772/38400 ◽  
2012 ◽  
Author(s):  
Irena Koutna ◽  
Pavel Simara ◽  
Petra Ondrackova ◽  
Lenka Tesarrov
Keyword(s):  
Flow Cytometry ◽  
Flow Cytometry Analysis ◽  
Intracellular Protein

scholarly journals A Synergic Potential of Antimicrobial Peptides against Pseudomonas syringae pv. actinidiae

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1461
Author(s):  
Nuno Mariz-Ponte ◽  
Laura Regalado ◽  
Emil Gimranov ◽  
Natália Tassi ◽  
Luísa Moura ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antimicrobial Peptides ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
High Efficiency ◽  
Bacterial Canker ◽  
Membrane Permeation ◽  
Flow Cytometry Analysis ◽  
Pathogenic Agent ◽  
Peptide Mixtures

Pseudomonas syringae pv. actinidiae (Psa) is the pathogenic agent responsible for the bacterial canker of kiwifruit (BCK) leading to major losses in kiwifruit productions. No effective treatments and measures have yet been found to control this disease. Despite antimicrobial peptides (AMPs) having been successfully used for the control of several pathogenic bacteria, few studies have focused on the use of AMPs against Psa. In this study, the potential of six AMPs (BP100, RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) to control Psa was investigated. The minimal inhibitory and bactericidal concentrations (MIC and MBC) were determined and membrane damaging capacity was evaluated by flow cytometry analysis. Among the tested AMPs, the higher inhibitory and bactericidal capacity was observed for BP100 and CA-M with MIC of 3.4 and 3.4–6.2 µM, respectively and MBC 3.4–10 µM for both. Flow cytometry assays suggested a faster membrane permeation for peptide 3.1, in comparison with the other AMPs studied. Peptide mixtures were also tested, disclosing the high efficiency of BP100:3.1 at low concentration to reduce Psa viability. These results highlight the potential interest of AMP mixtures against Psa, and 3.1 as an antimicrobial molecule that can improve other treatments in synergic action.

scholarly journals Flow cytometry analysis of anti-polyethylene glycol antibodies in human plasma

2021 ◽  
Vol 8 ◽  
pp. 148-154
Author(s):  
Jia-Long Fang ◽  
Frederick A. Beland ◽  
Yangshun Tang ◽  
Steve R. Roffler
Keyword(s):  
Flow Cytometry ◽  
Polyethylene Glycol ◽  
Human Plasma ◽  
Flow Cytometry Analysis

Flow cytometry analysis of peripheral Tregs in patients with multiple myeloma under lenalidomide maintenance

2021 ◽  
Author(s):  
Kenji Nozaki ◽  
Yuki Fujioka ◽  
Daisuke Sugiyama ◽  
Jun Ishikawa ◽  
Masato Iida ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Flow Cytometry ◽  
Flow Cytometry Analysis

scholarly journals Flow cytometry analysis of recombinantSaccharomyces cerevisiae populations

Cytometry ◽  
1986 ◽  
Vol 7 (2) ◽  
pp. 132-141 ◽  
Author(s):  
Friedrich Srienc ◽  
Judith L. Campbell ◽  
James E. Bailey
Keyword(s):  
Flow Cytometry ◽  
Flow Cytometry Analysis
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