scholarly journals Denaturing Gradient Gel Electrophoresis (DGGE) in Microbial Ecology - Insights from Freshwaters

10.5772/38177 ◽  
2012 ◽  
Author(s):  
Sofia Duarte ◽  
Fernanda Cassio ◽  
Claudia Pascoal
Keyword(s):  
Microbial Ecology ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gradient Gel Electrophoresis

Section 3 update: Denaturing gradient gel electrophoresis (DGGE) in microbial ecology

2008 ◽  
pp. 2645-2671 ◽  
Author(s):  
Gerard Muyzer ◽  
Thorsten Brinkhoff ◽  
Ulrich NÜbel ◽  
Cecilia Santegoeds ◽  
Hendrik SchÄfer ◽  
...  
Keyword(s):  
Microbial Ecology ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gradient Gel Electrophoresis

Microbial ecology of meat slicers as determined by denaturing gradient gel electrophoresis

Food Control ◽  
2014 ◽  
Vol 42 ◽  
pp. 242-247 ◽  
Author(s):  
Alexandria W. Mertz ◽  
Ok Kyung Koo ◽  
Corliss A. O'Bryan ◽  
Rubén Morawicki ◽  
Sujata A. Sirsat ◽  
...  
Keyword(s):  
Microbial Ecology ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gradient Gel Electrophoresis

scholarly journals Fluorophore-Labeled Primers Improve the Sensitivity, Versatility, and Normalization of Denaturing Gradient Gel Electrophoresis

2005 ◽  
Vol 71 (8) ◽  
pp. 4893-4896 ◽  
Author(s):  
Josh D. Neufeld ◽  
William W. Mohn
Keyword(s):  
Microbial Ecology ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Band Migration ◽  
Dgge Band ◽  
Gradient Gel Electrophoresis ◽  
Dgge Fingerprint ◽  
Fingerprint Detection

ABSTRACT Denaturing gradient gel electrophoresis (DGGE) is widely used in microbial ecology. We tested the effect of fluorophore-labeled primers on DGGE band migration, sensitivity, and normalization. The fluorophores Cy5 and Cy3 did not visibly alter DGGE fingerprints; however, 6-carboxyfluorescein retarded band migration. Fluorophore modification improved the sensitivity of DGGE fingerprint detection and facilitated normalization of samples from multiple gels by the application of intralane standards.

Allelopathic and Medicinal plant. 26. Millettia speciosa

2020 ◽  
Vol 51 (2) ◽  
pp. 125-146
Author(s):  
Nasiruddin Nasiruddin ◽  
Yu Zhangxin ◽  
Ting Zhao Chen Guangying ◽  
Minghui Ji
Keyword(s):  
Community Structure ◽  
Medicinal Plant ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wiener Index ◽  
Pseudomonas Spp ◽  
Evenness Index ◽  
Gradient Gel Electrophoresis ◽  
Rhizosphere Pseudomonas

We grew cucumber in pots in greenhouse for 9-successive cropping cycles and analyzed the rhizosphere Pseudomonas spp. community structure and abundance by PCR-denaturing gradient gel electrophoresis and quantitative PCR. Results showed that continuous monocropping changed the cucumber rhizosphere Pseudomonas spp. community. The number of DGGE bands, Shannon-Wiener index and Evenness index decreased during the 3rd cropping and thereafter, increased up to the 7th cropping, however, however, afterwards they decreased again. The abundance of Pseudomonas spp. increased up to the 5th successive cropping and then decreased gradually. These findings indicated that the structure and abundance of Pseudomonas spp. community changed with long-term cucumber monocropping, which might be linked to soil sickness caused by its continuous monocropping.

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