scholarly journals Immunocytochemistry in Early Mammalian Embryos

10.5772/37391 ◽  
2012 ◽  
Author(s):  
Hesam Dehghani
Keyword(s):  
Mammalian Embryos

Immunoprobe localization in mammalian embryos

1990 ◽  
Vol 48 (3) ◽  
pp. 32-33
Author(s):  
Patricia G. Calarco ◽  
Margaret C. Siebert
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Thin Sections ◽  
Relative Lack ◽  
Large Size ◽  
Mammalian Embryos ◽  
Antigen Localization ◽  
Difficult Challenge ◽  
Lowicryl K4m ◽  
Fertilized Egg

Visualization of preimplantation mammalian embryos by electron microscopy is difficult due to the large size of the ircells, their relative lack of internal structure, and their highly hydrated cytoplasm. For example, the fertilized egg of the mouse is a single cell of approximately 75μ in diameter with little organized cytoskelet on and apaucity ofor ganelles such as endoplasmic reticulum (ER) and Golgi material. Thus, techniques that work well on tissues or cell lines are often not adaptable to embryos at either the LM or EM level.Over several years we have perfected techniques for visualization of mammalian embryos by LM and TEM, SEM and for the pre-embedding localization of antigens. Post-embedding antigenlocalization in thin sections of mouse oocytes and embryos has presented a more difficult challenge and has been explored in LR White, LR Gold, soft EPON (after etching of sections), and Lowicryl K4M. To date, antigen localization has only been achieved in Lowicryl-embedded material, although even with polymerization at-40°C, the small ER vesicles characteristic of embryos are unrecognizable.

Regulation of nuclear size in mammalian embryos

2014 ◽  
Author(s):  
Elina Tsichlaki ◽  
Greg Fitzharris
Keyword(s):  
Nuclear Size ◽  
Mammalian Embryos

scholarly journals 3D structured illumination microscopy of mammalian embryos and spermatozoa

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Jens Popken ◽  
Maik Dahlhoff ◽  
Tuna Guengoer ◽  
Eckhard Wolf ◽  
Valeri Zakhartchenko
Keyword(s):  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Mammalian Embryos

Some significant steps in the cryopreservation of mammalian embryos with a note on a vitrification procedure

1989 ◽  
Vol 19 (1-2) ◽  
pp. 117-129 ◽  
Author(s):  
A. Massip ◽  
P. Van Der Zwalmen ◽  
B. Scheffen ◽  
F. Ectors
Keyword(s):  
Mammalian Embryos

Experimental Demonstration of Tongue Muscle Origin in Chick Embryos

Development ◽  
1958 ◽  
Vol 6 (4) ◽  
pp. 527-529
Author(s):  
E. M. Deuchar
Keyword(s):  
Chick Embryos ◽  
Occipital Region ◽  
Experimental Demonstration ◽  
Tongue Muscle ◽  
Segmental Nerve ◽  
Reptile Species ◽  
Tongue Muscles ◽  
Mammalian Embryos ◽  
Muscle Origin

Since in all classes of vertebrates the tongue muscles are innervated by nerve XII, a segmental nerve of the occipital region, it is usually argued on this criterion alone that they originate from occipital myotome tissue. Descriptive evidence in support of this generalization is, however, far from adequate. The most complete accounts that exist refer to one amphibian and two reptile species. In the amphibian Necturus, Platt (1897) observed that ventral outgrowths of the 3rd and 4th occipital myotomes became tongue muscles, and Edgeworth (1935) has described the development of tongue muscles in the reptiles Sphenodon and Lacerta, from ventral parts of two occipital and two cervical myotomes, all innervated by nerve XII. In avian and mammalian embryos, however, early muscle rudiments are extremely difficult to recognize with any certainty histologically.

A programmed oxyradical burst causes hatching of mouse blastocysts

1997 ◽  
Vol 110 (14) ◽  
pp. 1597-1602
Author(s):  
M. Thomas ◽  
S. Jain ◽  
G.P. Kumar ◽  
M. Laloraya
Keyword(s):  
Zona Pellucida ◽  
High Quantum ◽  
Short Period ◽  
Mammalian Embryos

The emergence of the mammalian blastocysts from their thick glycoprotein investment known as the zona pellucida is an important, but poorly understood, event in embryogenesis. In this paper, we demonstrate that peri-hatching blastocysts generate a considerably high quantum of an active oxyradical species for an extremely short period of time when compared to the pre-hatching (unhatched) and post-hatching (hatched) blastocysts. Hatching could be induced in pre-hatching blastocysts by exposing them to superoxide artificially generated to match the observed peri-implantation stage specific levels of superoxide, without impairing their viability. These observations suggest the operation of a superoxide-dependent hatching initiation in developing mammalian embryos.

CULTURES OF GONADS OF MAMMALIAN EMBRYOS

1959 ◽  
Vol 14 (3) ◽  
pp. 450
Author(s):  
EDWARD A. HOLYOKE ◽  
BERNARD A. BEBER
Keyword(s):  
Mammalian Embryos
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