scholarly journals Computer-Assisted High Tibial and Double Level Osteotomies for Genu Varum Deformity

Author(s):  
Dominique Saragaglia ◽  
Sam Hakki
Keyword(s):  
2017 ◽  
Vol 65 (3) ◽  
pp. 133-138
Author(s):  
Dominique Saragaglia ◽  
René-Christopher Rouchy ◽  
Jérémy Cognault

2009 ◽  
Vol 34 (2) ◽  
pp. 185-190 ◽  
Author(s):  
Dominique Saragaglia ◽  
Numa Mercier ◽  
Pierre-Emmanuel Colle
Keyword(s):  

2011 ◽  
Vol 36 (5) ◽  
pp. 999-1003 ◽  
Author(s):  
Dominique Saragaglia ◽  
Marc Blaysat ◽  
Numa Mercier ◽  
Mathieu Grimaldi
Keyword(s):  

2006 ◽  
Vol 5 (4) ◽  
pp. 212-217
Author(s):  
Dominique Saragaglia ◽  
Jason Roberts ◽  
Brice Rubens-Duval
Keyword(s):  

2008 ◽  
Vol 16 (2) ◽  
pp. 91-96 ◽  
Author(s):  
Dominique Saragaglia ◽  
Charbel Nemer ◽  
Pierre-Emmanuel Colle
Keyword(s):  

Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.


Author(s):  
A.M. Jones ◽  
A. Max Fiskin

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.


Author(s):  
Beverly L. Giammara ◽  
Jennifer S. Stevenson ◽  
Peggy E. Yates ◽  
Robert H. Gunderson ◽  
Jacob S. Hanker

An 11mm length of sciatic nerve was removed from 10 anesthetized adult rats and replaced by a biodegradable polyester Vicryl™ mesh sleeve which was then injected with the basement membrane gel, Matrigel™. It was noted that leg sensation and movement were much improved after 30 to 45 days and upon sacrifice nerve reconnection was noted in all animals. Epoxy sections of the repaired nerves were compared with those of the excised segments by the use of a variation of the PAS reaction, the PATS reaction, developed in our laboratories for light and electron microscopy. This microwave-accelerated technique employs periodic acid, thiocarbohydrazide and silver methenamine. It stains basement membrane or Type IV collagen brown and type III collagen (reticulin), axons, Schwann cells, endoneurium and perineurium black. Epoxy sections of repaired and excised nerves were also compared by toluidine blue (tb) staining. Comparison of the sections of control and repaired nerves was done by computer-assisted microscopic image analysis using an Olympus CUE-2 Image Analysis System.


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