scholarly journals PCR-RFLP and Real-Time PCR Techniques in Molecular Cancer Investigations

10.5772/36866 ◽  
2012 ◽  
Author(s):  
Uzay Gormus ◽  
Nur Selvi ◽  
Ilhan Yaylim-Eralt
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Rflp ◽  
Pcr Techniques

scholarly journals Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattle

2013 ◽  
Vol 58 (No. 12) ◽  
pp. 605-608
Author(s):  
P. Kumar ◽  
BL Jangir ◽  
G. Saikumar ◽  
R. Somvanshi
Keyword(s):  
Dairy Cattle ◽  
Real Time ◽  
Real Time Pcr ◽  
Rice Grain ◽  
Bovine Papillomavirus ◽  
Viral Dna ◽  
Specific Primers ◽  
Copy Numbers ◽  
Pcr Techniques

The present study was carried out to investigate the involvement of different bovine papillomaviruses in the teat warts of cattle. A total of 11 teat wart samples showing rice grain-like and small, sessile elevated greyish or flesh-like growths were collected from dairy cattle. DNA was extracted from these teat wart samples and PCR and real time PCR techniques were applied using specific primers for BPV-1 and -10 to detect the presence of viral nucleic acid. PCR revealed the presence of viral DNA of BPV-1 and -10 in three and seven samples, respectively. Quantification using real time PCR revealed that the copy numbers of the viral DNA of BPV-1 and -10 DNA varied from 1.12E + 04 to 2.99E + 04 and 3.56E + 02 to 5.23E + 06, respectively. From the present study it can be concluded that BPV-1 and -10 are involved in production of rice grain-like and sessile elevated growths on the teats of cattle.

Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques

2012 ◽  
Vol 45 (2) ◽  
pp. 147-156 ◽  
Author(s):  
E. E. Andronov ◽  
S. N. Petrova ◽  
A. G. Pinaev ◽  
E. V. Pershina ◽  
S. Zh. Rakhimgalieva ◽  
...  
Keyword(s):  
Microbial Community ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Techniques

scholarly journals Detection of a new case harboring mitochondrial A3243G mutation of MELAS syndrome

2017 ◽  
Vol 33 (1S) ◽  
Author(s):  
Phung Bao Khanh ◽  
Nguyen Minh Hoang ◽  
Pham Van Anh ◽  
Le Ngoc Anh ◽  
Cao Vu Hung ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Lactic Acidosis ◽  
Real Time ◽  
Mutation Analysis ◽  
Real Time Pcr ◽  
Melas Syndrome ◽  
Mitochondrial Encephalopathy ◽  
Pcr Rflp ◽  
A3243g Mutation ◽  
Severity Of The Disease

Mitochondrial genome A3243G mutation in the tRNALeu(UUR)  encodinggene (MTTL)is the main cause of mitochondrial encephalopathy, lactic acidosis and stroke-like episodes (MELAS). This mutation exists in heteroplasmic form and severity of the disease is affected by many factors including heteroplasmy level. In this study, a pediatric proband (female, 8 years old) was found to carry A3243G mutation at 77.6% of heteroplasmy by using PCR-RFLP in combination with real-time PCR. The results of  the A3243G mutation analysis of the proband’s family showed that her mother without any symptoms of encephalopathyalso carried the mutation at 7.9% of heteroplasmy whereas the mutation was not found in the proband’s healthy father and healthy sister, indicating that the proband received the A3243G mutation from her mother and the expression of MELAS syndromes depended on the level of heteroplasmy.

scholarly journals Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

2016 ◽  
Vol 55 (2) ◽  
pp. 526-534 ◽  
Author(s):  
Ciro Martins Gomes ◽  
Mariana Vicente Cesetti ◽  
Natália Aparecida de Paula ◽  
Sebastián Vernal ◽  
Gaurav Gupta ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sybr Green ◽  
Conventional Pcr ◽  
Content Type ◽  
Tegumentary Leishmaniasis ◽  
Precise Diagnosis ◽  
American Tegumentary Leishmaniasis ◽  
Pcr Techniques ◽  
Composite Reference Standard

ABSTRACTThe precise diagnosis of American tegumentary leishmaniasis (ATL) is an essential task due to the disease's associated morbidity. A noninvasive, extremely sensitive, and highly specific exam is critical, particularly for mucosal leishmaniasis (ML), in which a low parasite quantity is expected. We aimed to compare the diagnostic accuracy of swab and biopsy sample analysis using SYBR Green- and TaqMan-based real-time PCR (qPCR) assays with that of a composite reference standard consisting of the Montenegro skin test, serology, histopathology, smears, culture, and conventional PCR. In total, 55 patients with ATL (ML, 18 patients; cutaneous leishmaniasis [CL], 37 patients) and 36 patients without ATL were studied. qPCR analysis of swabs was more accurate when using SYBR Green (87.88%; 95% confidence interval [CI], 77.86 to 93.73 patients) than when using TaqMan (78.79%; 95% CI, 67.49 to 86.92%) (P= 0.031). SYBR Green (84.72%; 95% CI, 74.68 to 91.25%) was also more accurate than TaqMan (73.61%; 95% CI, 62.42 to 82.41%) for biopsy samples (P= 0.008). All qPCR methods were 100% specific. Swabs and biopsy specimens had similar sensitivity when using the same chemistry (P= 0.125 for SYBR Green andP= 0.625 for TaqMan). Moreover, qPCR achieved better performance than most existing techniques used for the diagnosis of ATL and also detected theLeishmaniaparasite in a greater proportion of patients than the associated histopathology, smear, culture, and conventional PCR techniques did. Swabs therefore represent a useful diagnostic tool because they not only are noninvasive but also can achieve an accuracy similar to that of biopsy samples. The high accuracy of SYBR Green-based qPCR may also reduce the requirement for associated parasitological tests for ATL diagnosis.

scholarly journals Early determination of fetal sex in goat a comparison between real time PCR and ultrasonography

2022 ◽  
Vol 951 (1) ◽  
pp. 012059
Author(s):  
S A Hussein ◽  
K M Karam
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Maternal Blood ◽  
Fetal Sex ◽  
Blood Specimens ◽  
High Level ◽  
Pcr Techniques ◽  
Very High ◽  
Genital Tubercle

Abstract The point of the current study is to assess the productivity of the real time PCR and ultrasound techniques in early determination of fetal sex in Iraqi singleton pregnant goats. Our investigation has been led in Iraq, Al-Diwanya city from 10/8/2020 – 15/1/2021. The examination incorporates 45 singleton pregnant Iraqi goats, which initially inspected by ultrasound to affirm pregnancy and to decide the fetal sex depending on the restriction of the genital tubercle of the goat fetuses, after that, blood specimens had been gathered from the jugular vein of all examined does to detect fetal sex by discovery of AMLX and SRY genes in the circling cells free fetal DNA (ccffDNA) in these maternal blood specimens by utilizing real time PCR. Our outcomes showed an exceptionally high level of accuracy in real time PCR in contrast with the ultrasound strategy. The outcomes were affirmed by the true fetal sex after parturition in the inspected does. The complete symptomatic rate were 51.11% (23/45) and 97.78% (44/45) for ultrasound and PCR strategies separately. The exactness level of genuine analyzed female and male caprine kidding were 58.33% (7/12), 48.48% (16/33), and 100% (12/12), 96.97% (32/33) for ultrasound and real time PCR techniques separately. While the exactness rates of the two techniques utilized in this investigation for early caprine fetal sexing in respect to early pregnancies periods analyzed uncovered 100% (13/13), 96.3% (26/27), 100% (5/5), and 61.54% (8/13), 40.74% (11/27), 80% (4/5) in early pregnancy periods (58-62, 63-67, 68-73) days for real time PCR and ultrasound strategies individually. In conclusion our outcomes revealed a huge predominant exactness and productivity in fetal sexing in Iraqi singleton pregnant does in early development periods, with very high accuracy in real time PCR in compare to ultrasound techniques.

Epidemics of schistosomiasis in military staff assigned to endemic areas: standard diagnostic techniques and the development of real-time PCR techniques

2013 ◽  
Vol 23 (2) ◽  
pp. 226-227
Author(s):  
E. Biance-Valero ◽  
F. De Laval ◽  
M. Delerue ◽  
H. Savini ◽  
S. Cheinin ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Diagnostic Techniques ◽  
Endemic Areas ◽  
Pcr Techniques

Inter-laboratory and inter-assay comparison on two real-time PCR techniques for quantification of PCV2 nucleic acid extracted from field samples

2009 ◽  
Vol 133 (1-2) ◽  
pp. 172-178 ◽  
Author(s):  
C.K. Hjulsager ◽  
L. Grau-Roma ◽  
M. Sibila ◽  
C. Enøe ◽  
L. Larsen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Field Samples ◽  
Pcr Techniques

scholarly journals Sampling methods for rumen microbial counts by Real-Time PCR techniques

2007 ◽  
Vol 6 (sup2) ◽  
pp. 788-791
Author(s):  
A. Chiariotti ◽  
F. Grandoni ◽  
B. Ferri ◽  
S. Puppo
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sampling Methods ◽  
Microbial Counts ◽  
Pcr Techniques
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