scholarly journals Immunoassay - A Standard Method to Study the Concentration of Peptide Hormones in Reproductive Tissues in vitro

10.5772/36100 ◽  
2012 ◽  
Author(s):  
Agnieszka Rak-Mardya ◽  
Anna Ptak ◽  
Ewa ucja
Keyword(s):  
Standard Method ◽  
Peptide Hormones ◽  
Reproductive Tissues

INHIBITION OF LIPOLYTIC ACTIVITY OF SYNTHETIC β1–24 AND β1–39 CORTICOTROPHIN BY ANTI-ACTH ANTISERUM

1966 ◽  
Vol 51 (1) ◽  
pp. 88-94 ◽  
Author(s):  
A. Villanueva ◽  
S. J. H. Ashcroft ◽  
J. P. Felber
Keyword(s):  
Guinea Pig ◽  
Lipolytic Activity ◽  
Peptide Hormones ◽  
Fat Pad ◽  
Double Antibody ◽  
Epididymal Fat ◽  
Antibody Complex ◽  
Radio Immunoassay ◽  
Antigen Antibody

ABSTRACT The synthetic ACTH peptides β1–39 and β1–24 stimulated lipolysis as determined by the rat epididymal fat pad in vitro. The stimulating effect of these peptides was diminished by prior incubation of the peptides with antibodies produced by the guinea-pig against ACTH. The stimulating effect of these hormones was also diminished by the double antibody system used in the radio-immunoassay of ACTH and other peptide hormones, in which incubation with antiserum is followed by precipitation of the antigen-antibody complex by rabbit anti-guinea-pig-γ-globulin.

In vitro metabolism of glucose by bovine reproductive tissues obtained during the estrous cycle and after calving

1992 ◽  
Vol 70 (5) ◽  
pp. 1496-1508 ◽  
Author(s):  
C. C. Chase ◽  
R. P. Del Vecchio ◽  
S. B. Smith ◽  
R. D. Randel
Keyword(s):  
Estrous Cycle ◽  
In Vitro Metabolism ◽  
Reproductive Tissues

scholarly journals Comparative analyses of angiosperm secretomes identify apoplastic pollen tube functions and novel secreted peptides

2020 ◽  
Author(s):  
María Flores-Tornero ◽  
Lele Wang ◽  
David Potěšil ◽  
Said Hafidh ◽  
Frank Vogler ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Turgor Pressure ◽  
Pollen Grains ◽  
Pollen Tubes ◽  
Secreted Proteins ◽  
Reproductive Tissues ◽  
Small Proteins ◽  
Secreted Peptides

Abstract Key message Analyses of secretomes of in vitro grown pollen tubes from Amborella, maize and tobacco identified many components of processes associated with the cell wall, signaling and metabolism as well as novel small secreted peptides. Abstract Flowering plants (angiosperms) generate pollen grains that germinate on the stigma and produce tubes to transport their sperm cells cargo deep into the maternal reproductive tissues toward the ovules for a double fertilization process. During their journey, pollen tubes secrete many proteins (secreted proteome or secretome) required, for example, for communication with the maternal reproductive tissues, to build a solid own cell wall that withstands their high turgor pressure while softening simultaneously maternal cell wall tissue. The composition and species specificity or family specificity of the pollen tube secretome is poorly understood. Here, we provide a suitable method to obtain the pollen tube secretome from in vitro grown pollen tubes of the basal angiosperm Amborella trichopoda (Amborella) and the Poaceae model maize. The previously published secretome of tobacco pollen tubes was used as an example of eudicotyledonous plants in this comparative study. The secretome of the three species is each strongly different compared to the respective protein composition of pollen grains and tubes. In Amborella and maize, about 40% proteins are secreted by the conventional “classic” pathway and 30% by unconventional pathways. The latter pathway is expanded in tobacco. Proteins enriched in the secretome are especially involved in functions associated with the cell wall, cell surface, energy and lipid metabolism, proteolysis and redox processes. Expansins, pectin methylesterase inhibitors and RALFs are enriched in maize, while tobacco secretes many proteins involved, for example, in proteolysis and signaling. While the majority of proteins detected in the secretome occur also in pollen grains and pollen tubes, and correlate in the number of mapped peptides with relative gene expression levels, some novel secreted small proteins were identified. Moreover, the identification of secreted proteins containing pro-peptides indicates that these are processed in the apoplast. In conclusion, we provide a proteome resource from three distinct angiosperm clades that can be utilized among others to study the localization, abundance and processing of known secreted proteins and help to identify novel pollen tube secreted proteins for functional studies.

scholarly journals Embryo Rescue in Wide Crosses in Arachis. 3. In vitro Culture of Peg Tips of A. hypogaea Selfs and Interspecific Hybrids1

1987 ◽  
Vol 14 (2) ◽  
pp. 70-74 ◽  
Author(s):  
J. P. Moss ◽  
H. T. Stalker
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Culture ◽  
Successful Treatment ◽  
Interspecific Hybrids ◽  
Embryo Rescue ◽  
Wide Crosses ◽  
Reproductive Tissues ◽  
Globular Stage ◽  
Callus Production

Abstract Embryo rescue in wide crosses in Arachis has only been achieved from culturing ovules excised from well developed pods, or from immature pods derived from flowers treated with gibberellic acid (GA) and which had embryos large enough to dissect without injury. The objective of this study was to determine whether reproductive tissues could grow in vitro without the need to dissect them from the peg tip and to determine the effects of GA application on flowers at the time of pollination, age of peg when cultured, and the presence of the peg meristem on reproductive growth, callus production, and peg elongation in vitro. Pegs elongated in culture only when the peg meristem was not removed. Ovules enlarged and grew out of the surrounding peg tissue in 3.8 to 32.8% of the cultures. Significantly more ovules grew when the peg meristem was removed (p < 0.01) and when 10- and 20- day-old pegs were cultured (p < 0.05). Overall, the most successful treatment for growth of ovules was treating flowers with GA at pollination and culturing without the peg meristem 10 days after pollination when 25.0 and 32.8% of all hybrid and selfed ovules, respectively, grew. Embryo growth was observed in an average of 8.4 and 17.6% of embryo sacs in hybrid and self peg tips, respectively, with several embryos reaching the globular stage after 21 days in vitro. This illustrates the potential for culturing young reproductive tissues of Arachis to recover interspecific hybrids.

Steroid Sulphation by Human Female Reproductive Tissues in vitro

1973 ◽  
Vol 1 (3) ◽  
pp. 757-759 ◽  
Author(s):  
EDITH MELVILLE ◽  
HANNELORE BRAUNSBERG
Keyword(s):  
Human Female ◽  
Reproductive Tissues

IN VITRO PROCESSING OF PLACENTAL PEPTIDE HORMONES BY SMOOTH MICROSOMES

1980 ◽  
Vol 343 (1 Precursor Pro) ◽  
pp. 69-75 ◽  
Author(s):  
Irving Boime ◽  
Malgorzata Bielinska ◽  
Gary Rogers ◽  
Tom Rucinsky
Keyword(s):  
Peptide Hormones ◽  
In Vitro Processing

STEROID AND PROSTAGLANDIN SECRETION BY THE CORPUS LUTEUM, ENDOMETRIUM AND EMBRYOS OF CYCLIC AND PREGNANT PIGS

1979 ◽  
Vol 82 (3) ◽  
pp. 425-428 ◽  
Author(s):  
J. WATSON ◽  
C. E. PATEK
Keyword(s):  
Corpus Luteum ◽  
Late Stage ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Reproductive Tissues ◽  
Prostaglandin F

Prostaglandin F2α (PGF2α) secreted by the reproductive tissues of the pig in vitro was measured and it was found that the levels secreted by the corpus luteum and endometrium of early pregnant sows were significantly lower than those secreted by tissues during the late stage of the oestrous cycle. They were, however, comparable to levels secreted by tissues from the mid-stage of the oestrous cycle. Embryos also secreted significant amounts of PGF2α. Secretion of progesterone and oestradiol by the corpora lutea of both cyclic and pregnant pigs fell within accepted limits but embryos were also found to secrete significant amounts of oestradiol. The results suggest that luteal maintenance in the early pregnant pig is unlikely to be directly due to reduced synthesis of PGF2α.

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