scholarly journals Plant Extracts: A Potential Tool for Controlling Animal Parasitic Nematodes

The Biosphere ◽  
10.5772/34783 ◽  
2012 ◽  
Author(s):  
Pedro Mendoza de Gives ◽  
Mara Eugenia Lpez Arellano ◽  
Enrique Libano ◽  
Liliana Aguilar
Keyword(s):  
Plant Extracts ◽  
Parasitic Nematodes ◽  
Potential Tool

Effects of four tanniferous plant extracts on thein vitroexsheathment of third-stage larvae of parasitic nematodes

Parasitology ◽  
2006 ◽  
Vol 132 (4) ◽  
pp. 545-554 ◽  
Author(s):  
D. BAHUAUD ◽  
C. MARTINEZ-ORTIZ DE MONTELLANO ◽  
S. CHAUVEAU ◽  
F. PREVOT ◽  
F. TORRES-ACOSTA ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Plant Extracts ◽  
Castanea Sativa ◽  
Nematode Species ◽  
Parasitic Nematodes ◽  
Rye Grass ◽  
Pine Tree ◽  
Third Stage

The anthelmintic properties of tanniferous plants and of their secondary metabolites represent one possible alternative to chemotherapy that is currently being explored as a means of achieving sustainable control of gastrointestinal nematodes in ruminants. Previousin vivoandin vitroresults suggest that tanniferous plants can have direct anti-parasitic effect against different stages of nematodes. However, the mode of action of the bioactive plant compounds remains obscure. The objectives of the current study were (1) to examine the hypothesis that extracts of tanniferous plants might interfere with the exsheathment of third-stage infective larvae (L3); (2) to assess the role of tannins in the process by examining the consequence of adding an inhibitor of tannins (polyethylene glycol: PEG) to extracts. The effects of 4 tanniferous plant extracts on exsheathment have been examined on L3 ofHaemonchus contortusandTrichostrongylus colubriformis. Artificial exsheathment was inducedin vitroby adding hypochloride solution to larval suspension. The evolution of exsheathment with time was measured by repeated observations at 10-min interval for 60 min. The selected plants were: genista (Sarothamnus scoparius), heather (Erica erigena), pine tree (Pinus sylvestris), and chestnut tree (Castanea sativa), with tannin contents ranging from 1·5 to 24·7% of DM. Extracts of a non-tanniferous plant (rye grass, tannin content: 0·3% of DM) were included in the assay as negative controls. The extracts were tested at the concentration of 600 μg/ml and the effects were compared to the rate of exsheathment of control larvae in PBS. No statistical differences in the pattern of exsheathment was observed after addition of rye grass or genista extracts for both nematode species and with heather extracts forT. colubriformis. In contrast, pine tree extracts on larvae of both species and heather extracts withH. contortusinduced a significant delay in exsheathment. Last, contact with chest nut extracts led to a total inhibition of the process for both nematodes. These results suggest that extracts of tanniferous plants might affect a key process in the very early stages of larval invasion of the host. In most cases, the addition of PEG led to a total or partial restoration towards control values. This suggests that tannins are largely involved in the inhibitory process. However, other secondary metabolites may also interfere with the process that would help to explain some of the differences in response observed between the two nematode species.

Effect of the green manures Sinapis alba, Brassica napus and Raphanus sativus on hatching of Globodera rostochiensis

Nematology ◽  
2011 ◽  
Vol 13 (8) ◽  
pp. 965-975 ◽  
Author(s):  
Yirina Valdes ◽  
Roland N. Perry ◽  
Nicole Viaene ◽  
Maurice Moens
Keyword(s):  
Plant Extracts ◽  
Cover Crops ◽  
Globodera Rostochiensis ◽  
Control Measures ◽  
Environmentally Benign ◽  
Parasitic Nematodes ◽  
Plant Parasitic Nematodes ◽  
Green Manures ◽  
Important Pest

AbstractThe potato cyst nematode, Globodera rostochiensis, is a quarantine organism. Environmentally benign control measures for this economically important pest are needed. Green manures, in particular plants from the Brassicaceae, suppress some plant-parasitic nematodes and have potential as control agents. This study examined if growing and incorporating cover crops from the Brassicaceae family influenced hatching of G. rostochiensis. The effect of root diffusates and plant extracts, as well as soil incorporation of plant material from three commonly used species of green manures, was studied in in vitro bioassays and pot tests. The results showed that brassica diffusates and plant extracts were not nematicidal. In addition, although they did not cause hatch by themselves, pretreatment with these solutions enhanced subsequent hatch in host root diffusates. The results are discussed in the context of the hatching response of G. rostochiensis and the likely influence on field usage of these green manures.

scholarly journals Nematode Population Dynamics after Applications of Plant Extracts and Trichoderma Species as Soil Amendments in Tomato Field

2020 ◽  
Vol 9 (1) ◽  
pp. 43-49
Author(s):  
Timothy I. Olabiyi ◽  
Samuel O. Akinrinola ◽  
Olajumoke E. Ayanda
Keyword(s):  
Plant Extracts ◽  
Azadirachta Indica ◽  
Nematode Population ◽  
Growth And Yield ◽  
Parasitic Nematodes ◽  
Plant Parasitic Nematodes ◽  
Trichoderma Species ◽  
Tomato Field ◽  
And Control ◽  
Plant Parasitic

Three different plant extracts (Khaya ivorensis, Azadirachta indica and Daniella oleifera) and two Trichoderma species (T. harzianum and T. atroviride), singly and in combination, were assessed on nematode population changes and also in the control of plant parasitic nematodes on tomato under field condition during 2018 and 2019 planting seasons. During each trial, plant parasitic nematodes encountered in the field were Meloidogyne, Pratylenchus, Xiphinema and Helicotylenchus species. The experiment was a randomized complete block design with 4 x 3 factorial scheme and five replications. Factor A being plant extracts (Khaya ivorensis, Azadirachta indica, Daniella oleifera) and control, and factor B being bio-control agents (Trichoderma harzianum, T. atroviride) and control, resulting in 12 treatments. Significant reductions were observed on the population of the plant parasitic nematodes at all treatment levels with neem plant extract, in combination with either T. harzianum or T. atroviride resulted in the greatest significant decrease. Applications of different plant extracts and Trichoderma species, singly and in combination, enhanced the growth and yield of tomato infected with parasitic nematodes.

Plants producing pyrrolizidine alkaloids: sustainable tools for nematode management?

Nematology ◽  
2010 ◽  
Vol 12 (1) ◽  
pp. 1-24 ◽  
Author(s):  
Michael Boppré ◽  
Tim Thoden
Keyword(s):  
Plant Species ◽  
Plant Extracts ◽  
Pyrrolizidine Alkaloids ◽  
Nematode Species ◽  
Parasitic Nematodes ◽  
Plant Metabolites ◽  
Secondary Plant Metabolites ◽  
Nematode Management

Abstract1,2-dehydropyrrolizidine ester alkaloids (pyrrolizidine alkaloids; PAs) are a class of secondary plant metabolites found in hundreds of plant species. In vitro, PAs are known to affect plant-parasitic nematodes. Thus, PA-producing plants might be used in nematode management. So far, however, Crotalaria is the only PA-producing plant that has been used for nematode control. Consequently, we reviewed the literature on other PA plants and their effects on nematodes. This included the analysis of in vitro experiments with PA plant extracts, host tests with PA plants, as well as experiments in which PA-producing plants were used as different types of soil amendments (mulch, in situ mulching, extracts). Our results clearly show that, in addition to Crotalaria, certain other PA-producing plant species have the potential to be used in sustainable nematode management. This is especially true for management of sedentary endoparasitic nematode species. Promising applications include the use of PA plant extracts and mulches, as well as the use of PA plants for in situ mulching.

scholarly journals Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes

Parasitology ◽  
2007 ◽  
Vol 134 (12) ◽  
pp. 1831-1838 ◽  
Author(s):  
G. STEPEK ◽  
R. H. C. CURTIS ◽  
B. R. KERRY ◽  
P. R. SHEWRY ◽  
S. J. CLARK ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Specific Inhibitor ◽  
Parasitic Nematodes ◽  
Cysteine Proteinases ◽  
Plant Parasitic Nematodes ◽  
Second Stage ◽  
Anthelmintic Efficacy ◽  
Plant Parasitic

SUMMARYCysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera Meloidogyne and Globodera. All the plant extracts examined caused digestion of the cuticle and decreased the activity of the tested nematodes. The specific inhibitor of cysteine proteinases, E-64, blocked this activity completely, indicating that it was essentially mediated by cysteine proteinases. In vitro, plant cysteine proteinases are active against second-stage juveniles of M. incognita and M. javanica, and some cysteine proteinases also affect the second-stage juveniles of Globodera rostochiensis. It is not known yet whether these plant extracts will interfere with, or prevent invasion of, host plants.

scholarly journals Chemical Characterization of Plant Extracts and Evaluation of their Nematicidal and Phytotoxic Potential

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2216
Author(s):  
Raúl Velasco-Azorsa ◽  
Héctor Cruz-Santiago ◽  
Ignacio Cid del Prado-Vera ◽  
Marco Vinicio Ramirez-Mares ◽  
María del Rocío Gutiérrez-Ortiz ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Chemical Characterization ◽  
Parasitic Nematodes ◽  
The European Union ◽  
Plant Parasitic Nematodes ◽  
Second Stage ◽  
Seed Growth ◽  
Alternative Sources

Nacobbus aberrans ranks among the “top ten” plant-parasitic nematodes of phytosanitary importance. It causes significant losses in commercial interest crops in America and is a potential risk in the European Union. The nematicidal and phytotoxic activities of seven plant extracts against N. aberrans and Solanum lycopersicum were evaluated in vitro, respectively. The chemical nature of three nematicidal extracts (EC50,48h ≤ 113 µg mL−1) was studied through NMR analysis. Plant extracts showed nematicidal activity on second-stage juveniles (J2): (≥87%) at 1000 µg mL−1 after 72 h, and their EC50 values were 71.4–468.1 and 31.5–299.8 µg mL−1 after 24 and 48 h, respectively. Extracts with the best nematicidal potential (EC50,48h < 113 µg mL−1) were those from Adenophyllum aurantium, Alloispermum integrifolium, and Tournefortia densiflora, which inhibited L. esculentum seed growth by 100% at 20 µg mL−1. Stigmasterol (1), β-sitosterol (2), and α-terthienyl (3) were identified from A. aurantium, while 1, 2, lutein (4), centaurin (5), patuletin-7-β-O-glucoside (6), pendulin (7), and penduletin (8) were identified from A. integrifolium. From T. densiflora extract, allantoin (9), 9-O-angeloyl-retronecine (10), and its N-oxide (11) were identified. The present research is the first to report the effect of T. densiflora, A. integrifolium, and A. aurantium against N. aberrans and chemically characterized nematicidal extracts that may provide alternative sources of botanical nematicides.

Hemolysis of erythrocytes by the hemolytic system from the blood-feeding stable fly, Stomoxys calcitrans

1992 ◽  
Vol 50 (1) ◽  
pp. 690-691
Author(s):  
H. J. Kirch ◽  
G. Spates ◽  
R. Droleskey ◽  
W.J. Kloft ◽  
J.R. DeLoach
Keyword(s):  
Blood Feeding ◽  
Stomoxys Calcitrans ◽  
Stable Fly ◽  
Digestive Physiology ◽  
Transmission Electron ◽  
Erythrocyte Lysis ◽  
Mammalian Blood ◽  
Bovine Erythrocytes ◽  
Potential Tool

Blood feeding insects have to rely on the protein content of mammalian blood to insure reproduction. A substantial quantity of protein is provided by hemoglobin present in erythrocytes. Access to hemoglobin is accomplished only via erythrocyte lysis. It has been shown that midgut homogenates from the blood feeding stable fly, Stomoxys calcitrans, contain free fatty acids and it was proposed that these detergent-like compounds play a major role as hemolysins in the digestive physiology of this species. More recently sphingomyelinase activity was detected in midgut preparations of this fly, which would provide a potential tool for the enzymatic cleavage of the erythrocyte's membrane sphingomyelin. The action of specific hemolytic factors should affect the erythrocyte's morphology. The shape of bovine erythrocytes undergoing in vitro hemolysis by crude midgut homogenates from the stable fly was examined by scanning and transmission electron microscopy.

Inhibitory Measures of Attention as a Potential Tool for ADHD Diagnosis

1999 ◽  
Author(s):  
Heather Droste ◽  
Michelle Pattavina ◽  
Chris Smith ◽  
Robert Tigner
Keyword(s):  
Adhd Diagnosis ◽  
Potential Tool
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