scholarly journals Intraerythrocytic Plasmodium falciparum Growth in Serum-Free Medium with an Emphasis on Growth-Promoting Factors

10.5772/33212 ◽  
2012 ◽  
Author(s):  
Hiroko Asahi
Keyword(s):  
Plasmodium Falciparum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Growth Promoting

Continuous cultivation of intraerythrocytic Plasmodium falciparum in a serum-free medium with the use of a growth-promoting factor

Parasitology ◽  
1994 ◽  
Vol 109 (4) ◽  
pp. 397-401 ◽  
Author(s):  
H. Asahi ◽  
T. Kanazawa
Keyword(s):  
Plasmodium Falciparum ◽  
Bovine Serum ◽  
Basal Medium ◽  
Parasite Growth ◽  
Serum Free Medium ◽  
Free Medium ◽  
Commercial Preparation ◽  
Serum Free ◽  
Adult Bovine Serum ◽  
Growth Promoting

SUMMARYSerum-free media were used to culture Plasmodium falciparum. A commercial preparation, Daigo's GF21 developed as a growth-promoting factor for many kinds of mammalian cells, and consisting of the 55–70% ammonium sulphate fraction of adult bovine serum, insulin, transferrin, ethanolamine and sodium selenite, was found to sustain growth of the parasite when Daigo's T was employed as a basal medium. The optimal Daigo's GF21 concentration for parasite growth was between 5 and 20% (v/v), with the best results at 10%. Differential counts indicated that Daigo's GF21 is essential for schizogony. Established serum-free medium, GIT, consisting of Daigo's T basal medium and Daigo's GF21, yielded good parasite growth without any supplementation. Growth-promoting factor derived from adult bovine serum in Daigo's GF21 was shown to be crucial to parasite growth. The results presented here will not only be of practical value, but will provide important information about the developmental requirements for the parasite.

Plasmodium falciparum: Differing effects of non-esterified fatty acids and phospholipids on intraerythrocytic growth in serum-free medium

2011 ◽  
Vol 127 (3) ◽  
pp. 708-713 ◽  
Author(s):  
Hiroko Asahi ◽  
Shinji Izumiyama ◽  
Mohammed Essa Marghany Tolba ◽  
Bethel Kwansa-Bentum
Keyword(s):  
Fatty Acids ◽  
Plasmodium Falciparum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Esterified Fatty Acids

Determination of Fifty Percent Inhibitory Concentrations (IC50) of Antimalarial Drugs against Plasmodium Falciparum Parasites in a Serum-Free Medium

1994 ◽  
Vol 51 (2) ◽  
pp. 214-218 ◽  
Author(s):  
Ayub O. Ofulla ◽  
Gladys M. Aleman ◽  
Aloys S. Orago ◽  
John I. Githure ◽  
Anthony J. Johnson ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antimalarial Drugs ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

P82 Continuous cultivation of Babesia caballi in a serum-free medium with the use of a growth-promoting factor

2001 ◽  
Vol 52 (Supplement) ◽  
pp. 98
Author(s):  
Hiromi IKADAI ◽  
MUINDE D. Martin ◽  
Ikuo IGARASHI ◽  
Hideyuki NAGASAWA ◽  
Kozo FUJISAKI ◽  
...  
Keyword(s):  
Continuous Cultivation ◽  
Serum Free Medium ◽  
Free Medium ◽  
Babesia Caballi ◽  
Serum Free ◽  
Growth Promoting

Cultivation of Plasmodium falciparum Parasites in a Serum-Free Medium

1993 ◽  
Vol 49 (3) ◽  
pp. 335-340 ◽  
Author(s):  
Ayub V. O. Ofulla ◽  
Baldip Khan ◽  
Anthony J. Johnson ◽  
John I. Githure ◽  
Samuel K. Martin ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free
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