Centromere Evolution: Digging into Mammalian Primary Constriction

Dissociation of minor satellite from the centromere in mouse

Journal of Cell Science ◽

10.1242/jcs.107.11.3091 ◽

1994 ◽

Vol 107 (11) ◽

pp. 3091-3095

Author(s):

B.K. Vig ◽

D. Latour ◽

J. Frankovich

Keyword(s):

Satellite Dna ◽

Hoechst 33258 ◽

Major Satellite ◽

Primary Constriction ◽

Minor Satellite ◽

Antibody Reaction

The minor satellite DNA of mouse is believed to constitute the centromere. We report that centromeres of some chromosomes in the Cl1D cells of mouse are not associated with this DNA even though the latter is present on these chromosomes. The satellite DNA was detected distally from the centromere and could not be mistaken as a component of the centromere. We also report that the site of the primary constriction may not always coincide with the site of the anti-kinetochore antibody reaction. Whereas the regions containing the major satellite decondense upon treatment with bisbenzimidazole (Hoechst 33258), the sites carrying minor satellite resist decondensing.

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Primary Constriction

10.1007/springerreference_36411 ◽

2011 ◽

Keyword(s):

Primary Constriction

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Centromere (Primary Constriction)

Dictionary of Bioinformatics and Computational Biology ◽

10.1002/9780471650126.dob0087.pub2 ◽

2004 ◽

Author(s):

Katheleen Gardiner

Keyword(s):

Primary Constriction

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Probing The Mechanisms Underlying Kinetochore Behavior In Vertebate Cells Using Combinations of Advanced Light and 3-D Electron Microscopy

Microscopy and Microanalysis ◽

10.1017/s1431927600007972 ◽

1997 ◽

Vol 3 (S2) ◽

pp. 217-218

Author(s):

B. F. McEwen ◽

A.B. Heagle ◽

C.L. Rieder

Keyword(s):

Electron Microscopy ◽

Mitotic Spindle ◽

Attachment Site ◽

Spindle Pole ◽

Nuclear Envelope Breakdown ◽

Daughter Cells ◽

Primary Constriction ◽

Sister Kinetochore ◽

To Receive ◽

Chromosome Motion

For daughter cells to receive equal copies of the genome during mitosis, the replicated chromosomes must attach to and move bi-directionally on the mitotic spindle. A chromosome becomes attached to the spindle via a pair specialized structures, known as kinetochores, that are positioned on opposite sides of its primary constriction (one on each of the two chromatids). In addition to being the spindle attachment site, kinetochores are also involved in producing and/or transmitting the forces for chromosome motion. In vertebrates the kinetochore closest to a spindle pole at the time of nuclear envelope breakdown usually is the first to attach to the spindle. As a result of this attachment the now “monooriented” chromosome moves toward the closest pole where its only attached kinetochore initiates oscillatory motions toward and away from that pole until the unattached sister kinetochore acquires microtubules (Mts) from the opposite spindle pole.

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