scholarly journals Pattern of Circulating Mycobacterium tuberculosis Strains in Sri Lanka

10.5772/32166 ◽  
2012 ◽  
Author(s):  
Dhammika Magana-Arachchi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sri Lanka

scholarly journals Insight into genetic diversity of Mycobacterium tuberculosis in Kandy, Sri Lanka reveals predominance of the Euro-American lineage

2019 ◽  
Vol 87 ◽  
pp. 84-91 ◽  
Author(s):  
Charitha Mendis ◽  
Vasanthi Thevanesam ◽  
Athula Kumara ◽  
Susiji Wickramasinghe ◽  
Dushantha Madegedara ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Sri Lanka ◽  
Insight Into

scholarly journals Genetic diversity of Mycobacterium tuberculosis isolates obtained from three distinct population groups in the Central Province, Sri Lanka

2015 ◽  
Vol 5 (5) ◽  
pp. 385-392 ◽  
Author(s):  
Dulanthi Weerasekera ◽  
Dhammika Magana-Arachchi ◽  
Dushantha Madegedara ◽  
Neranjan Dissanayake ◽  
Vasanthi Thevanesam
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Sri Lanka ◽  
Central Province ◽  
Distinct Population ◽  
Population Groups

scholarly journals RFLP clusters of rifampicin resistant and susceptible Mycobacterium tuberculosis strains in Western province of Sri Lanka

2017 ◽  
Vol 11 (08) ◽  
pp. 619-625
Author(s):  
Chamila Priyangani Adikaram ◽  
Sandya Sulochana Wijesundera ◽  
Jennifer Perera
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Sri Lanka ◽  
Novel Mutation ◽  
Point Mutations ◽  
Clonal Expansion ◽  
Rpob Gene ◽  
The Novel ◽  
Western Province ◽  
Control And Prevention

Introduction: Continuous studies on genetic diversity of Mycobacterium tuberculosis could enhance the awareness on transmission, control and prevention of tuberculosis (TB). In this study, we investigated current genetic diversity of TB and rifampicin resistant TB by, Restriction Fragment Length Polymorphism (RFLP) based on fingerprinting of the IS6110 insertion sequence, in the Western province of Sri Lanka, the famous touristic destination with the highest TB burden in the country. Methodology: Genomic DNA extracted from susceptible and rifampicin resistant TB strains (confirmed for rpoB gene point mutations) were digested with PvuII restriction enzyme, electrophoresed and subjected to Southern transfer. The blots were hybridised with IS6110 probe and visualized using a chemiluminescence detection. Results: The number of copies of IS6110 per isolate varied from 1 to 14. The dendrogram revealed a total of 68 distinct strains among 77 TB isolates and they belonged to nine clusters. Both rifampicin resistant and susceptible strains were distributed in all clusters. This evaluation revealed the absence of genetically identical or strong relatedness between susceptible and resistant isolates. However, clonal expansion was detected in transmission of both TB and rifampicin resistant TB. In addition, the resistant isolates having the novel mutation had no clonal relatedness. Conclusion: This is the first observational study regarding clonal expansion of TB in Sri Lanka. Thus, further investigation on genotypes, clonal expansion and transmission of drug resistance using additional markers would be useful for controlling TB.

scholarly journals Is International Travel an Emerging Issue on Transmission of Beijing Lineage Mycobacterium tuberculosis?

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Pavithra S. Madamarandawala ◽  
Srinath Satyanarayana ◽  
Collins Timire ◽  
Aashifa Yaqoob ◽  
Dushantha Madegedara ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sri Lanka ◽  
Treatment Success ◽  
International Travel ◽  
Sputum Smear ◽  
Success Rates ◽  
Specific Primers ◽  
Tourist Destination ◽  
Study Population ◽  
Polymerase Chain

Mycobacterium tuberculosis belonging to Beijing sublineage (BL) is associated with high tuberculosis (TB) transmission, multidrug resistance, and adverse treatment outcomes. Sri Lanka experiences an increase in the number of travellers/workers to and from high TB-burden countries, and there is risk of getting BL strains imported into the country. In this context, a cohort study was conducted to assess the prevalence of BL strains among pulmonary tuberculosis (PTB) patients in the Kandy district of Sri Lanka (a popular tourist destination) and its association with patients’ sociodemographic and clinical characteristics. The study population included sputum smear-positive PTB patients diagnosed from February 2018–July 2019. Fresh sputum samples were collected for culturing and conducted polymerase chain reaction using BL-specific primers. Among the 101 patients recruited, presence of BL strains could be ascertained in 94 patients of which 24 (26%; 95% CI: 18%–35%) had BL strains. Prevalence of BL strains was higher among those with high sputum smear grades (2+ and 3+) (P<0.05) and those who had travelled abroad (P<0.05). The prevalence was also higher among young people (aged <35 years). Treatment success rates were similar in patients with (83%) and without BL strains (83% vs. 81%; P value = 0.8375). The prevalence of BL strains in Kandy, Sri Lanka, was high compared to previously reported figures in Sri Lanka, and the percentage drives closer to the countries in South East Asia. International travel raises itself as an emerging issue in BL transmission urging the need of policies and practices in immigration/emigration strategies. The study findings have the potential to alter the TB epidemiology in the country and might represent the situation in other underexplored countries as well. Therefore, it is important to monitor the trends and factors related to the prevalence of Beijing strains globally and make decisions as a whole.

scholarly journals Coexistence of mycobacterial infections – Mycobacterium tuberculosis and Mycobacterium leprae – in Sri Lanka: a case series

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
B. S. D. P. Keragala ◽  
H. M. M. T. B. Herath ◽  
G. H. D. C. Janapriya ◽  
S. Vanitha ◽  
Thanushah Balendran ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sri Lanka ◽  
Case Series ◽  
Mycobacterium Leprae ◽  
Mycobacterial Infections
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