scholarly journals Ammonia Accumulation of Novel Nitrogen-Fixing Bacteria

10.5772/30654 ◽  
2012 ◽  
Author(s):  
Kenichi Iwata ◽  
San San ◽  
Nik Noor Azlin binti Azlan ◽  
Toshio Omori
Keyword(s):  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Ammonia Accumulation

scholarly journals ISOLATION, MOLECULAR CHARACTERIZATION OF DIAZOTROPIC BACTERIA FROM VIRGIN SOIL FROM ARAKU VALLEY AND ASSESSMENT OF OF AMMONIA ACCUMULATION AT SALINE CONDITIONS

2021 ◽  
Vol 9 (11) ◽  
pp. 824-832
Author(s):  
Simmanna Nakka ◽  
◽  
S. Uday Bhaskar ◽  
Keyword(s):  
Biochemical Characterization ◽  
Growth Parameters ◽  
Soil Samples ◽  
Virgin Soil ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Symbiotic Associations ◽  
Ammonia Accumulation ◽  
Agar Media

Nitrogen-fixing bacteria are widely distributed in nature where they reduce atmospheric nitrogen in soil or in association with plant. They have been found in a wide variety of terrestrial and aquatic habitats in both temperate and tropical regions of the word. Nitrogen-fixing bacteria are found in symbiotic associations with plants free living in soil. The objective of the present research was to isolate free living Nitrogen fixing bacteria from virgin soil samples in araku valley and assessment of their ammonia accumulation at saline conditions. 10 soil samples were collected in different place from virgin areas. For isolation of the free living nitrogen fixing bacteria, Nitrogen free media like Jensens Medium and Azotobacter Agar were used. Serially diluted soil samples were spread on the agar media and incubated for 48 hours. Eleven morphologically different bacteria were separated on made pure colonies on nutrient agar media. All bacteria were under go biochemical characterization which reveals that all these bacteria related to Azospirillum, Azotobacter and Clostridium. High ammonia liberating isolate MGN-10 was molecular characterizes as Azotobacter chroococcum and this soil application increase the plant growth in terms of growth parameters.

scholarly journals Cowpea symbiotic efficiency, pH and aluminum tolerance in nitrogen-fixing bacteria

2014 ◽  
Vol 71 (3) ◽  
pp. 171-180 ◽  
Author(s):  
Bruno Lima Soares ◽  
Paulo Avelar Ademar Ferreira ◽  
Silvia Maria de Oliveira-Longatti ◽  
Leandro Marciano Marra ◽  
Marcia Rufini ◽  
...  
Keyword(s):  
Aluminum Tolerance ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Symbiotic Efficiency

scholarly journals Genomic characterization and computational phenotyping of nitrogen-fixing bacteria isolated from Colombian sugarcane fields

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luz K. Medina-Cordoba ◽  
Aroon T. Chande ◽  
Lavanya Rishishwar ◽  
Leonard W. Mayer ◽  
Lina C. Valderrama-Aguirre ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Phosphate Solubilization ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Promising Tool ◽  
A Genome ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPrevious studies have shown the sugarcane microbiome harbors diverse plant growth promoting microorganisms, including nitrogen-fixing bacteria (diazotrophs), which can serve as biofertilizers. The genomes of 22 diazotrophs from Colombian sugarcane fields were sequenced to investigate potential biofertilizers. A genome-enabled computational phenotyping approach was developed to prioritize sugarcane associated diazotrophs according to their potential as biofertilizers. This method selects isolates that have potential for nitrogen fixation and other plant growth promoting (PGP) phenotypes while showing low risk for virulence and antibiotic resistance. Intact nitrogenase (nif) genes and operons were found in 18 of the isolates. Isolates also encode phosphate solubilization and siderophore production operons, and other PGP genes. The majority of sugarcane isolates showed uniformly low predicted virulence and antibiotic resistance compared to clinical isolates. Six strains with the highest overall genotype scores were experimentally evaluated for nitrogen fixation, phosphate solubilization, and the production of siderophores, gibberellic acid, and indole acetic acid. Results from the biochemical assays were consistent and validated computational phenotype predictions. A genotypic and phenotypic threshold was observed that separated strains by their potential for PGP versus predicted pathogenicity. Our results indicate that computational phenotyping is a promising tool for the assessment of bacteria detected in agricultural ecosystems.

scholarly journals Effect of Abandonment on Diversity and Abundance of Free-Living Nitrogen-Fixing Bacteria and Total Bacteria in the Cropland Soils of Hulun Buir, Inner Mongolia

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e106714 ◽  
Author(s):  
Huhe ◽  
Shinchilelt Borjigin ◽  
Yunxiang Cheng ◽  
Nobukiko Nomura ◽  
Toshiaki Nakajima ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Total Bacteria
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