scholarly journals Advances in Domestication and Culture Techniques for Crayfish Procambarus acanthophorus

Aquaculture ◽  
10.5772/28173 ◽  
2012 ◽  
Author(s):  
Martha P. ◽  
Carlos I.
Keyword(s):  
Culture Techniques

A T.E.M. study of mouse mammary epithelial cell secretory differentiation cultured on collagen and Matrigel

1991 ◽  
Vol 49 ◽  
pp. 188-189
Author(s):  
W.N. Bentham ◽  
V. Rocha
Keyword(s):  
Cell Culture ◽  
Mammary Epithelial ◽  
Secretory Process ◽  
Cell Culture System ◽  
Protein Maturation ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Mouse Mammary Epithelial Cell ◽  
Secretory Differentiation

It has been an interest of our lab to develop a mammary epethelial cell culture system that faithfully duplicates the in vivo condition of the lactating gland. Since the introduction of collagen as a matrix on which cells are cultivated other E.C.M. type matrices have been made available and are used in many cell culture techniques. We have previously demonstrated that cells cultured on collagen and Matrigel do not differentiate as they do in vivo. It seems that these cultures often produce cells that show a disruption in the secretory process. The appearance of large ribosomal studded vesicles, that specifically label with antibody to casein, suggest an interruption of both protein maturation and secretion at the E.R. to golgi transition. In this report we have examined cultures on collagen and Matrigel at relative high and low seeding densities and compared them to cells from the in vivo condition.

Dwindling status of Epimedium Elatum (Morren & Decne) and its geographical distribution in Kashmir Himalaya, India

2018 ◽  
pp. 47-52
Keyword(s):  
Medicinal Plant ◽  
Biological Activities ◽  
Conservation Status ◽  
Natural Populations ◽  
Culture Techniques ◽  
Kashmir Himalayas ◽  
Near Future ◽  
First Time ◽  
Tissue Culture Techniques

Epimedium elatum (Morren & Decne) of family Berberidaceace is a rare perennial medicinal plant, endemic to high altitude forests of Northwestern Himalayas in India. Ethnobotanically, it has been used as an ingredient for treatment of bone-joint disorders, impotence and kidney disorders in Kashmir Himalayas. Phytochemically, it is rich in Epimedin ABC and Icariin; all of these have been demonstrated to possess remarkable biological activities like PDE-5 inhibition (treatment of erectile dysfunction), anticancer, antiosteoporosis antioxidant and antiviral properties. The present investigation reports its traditional usage, comprehensive distribution and conservation status from twenty ecogeographical regions in Kashmir Himalayas, India. The species was reported from Gurez valley for the first time. Numerous threats like excessive grazing, deforestration, habitat fragmentation, tourism encroachment, landslides and excessive exploitation have decreased its natural populations in most of the surveyed habitats. Consequently, its existence may become threatened in near future if timely conservation steps are not taken immediately by concerned stakeholders involved in medicinal plant research. Moreover, use of plant tissue culture techniques is recommended for development of its in vitro propagation protocols. Therefore, introduction of this medicinal plant in botanical gardens, protected sites and development of monitoring programmes are needed for its immediate conservation in Northwestern Himalayas, India.

INTEGRATED ALGA-CULTURE IN INUNDATED COASTAL FARMLANDS OF INDIAN SUNDARBANS AS A SUSTAINABLE ADAPTATION FOR MARGINAL COMMUNITIES TOWARDS CLIMATE RISK REDUCTION

2017 ◽  
Author(s):  
Dipayan Dey ◽  
Dipayan Dey ◽  
Ashoka Maity ◽  
Ashoka Maity
Keyword(s):  
Coastal Wetlands ◽  
Algal Growth ◽  
Open Water ◽  
Fish Bone ◽  
Algal Flora ◽  
Daily Growth Rate ◽  
Monsoon Period ◽  
Daily Growth ◽  
Culture Techniques ◽  
Tidal Waters

Algae has a great potential for quick capture of biological carbon and its storage in saltwater-inundated coastal wetlands and can also be introduced as a climate adaptive alternate farming practice. An intervention with native algal flora Enteromorpha sp. in enclosed coastal Sundarbans in India on two open water culture techniques, viz. U-Lock & Fish-Bone, shows that growth in native algal stock is influenced by seasonal variations of salinity and other limnological factors. Sundarbans, facing the odds of climate change is fast loosing arable lands to sea level rise. Algaculture in inundated coastal areas can be an adaptive mitigation for the same. Perusal of results show that daily growth rate (DGR%) increases with increasing salinity of the intruding tidal waters to an extent and biomass increment under salt stress results in accumulation of metabolites those are having nutrient values and can yield bio-diesel as well. Algal growth recorded mostly in post monsoon period, has impacts on pH and Dissolved Oxygen (DO) of the ambient water to facilitate integrated pisciculture. The paper suggests that alga-culture has unrealized potentials in carbon sequestration and can be significantly used for extraction of Biodiesel.

scholarly journals Identification of Novel FNIN2 and FNIN3 Fibronectin-Derived Peptides That Promote Cell Adhesion, Proliferation and Differentiation in Primary Cells and Stem Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 3042
Author(s):  
Eun Ju Lee ◽  
Khurshid Ahmad ◽  
Shiva Pathak ◽  
SunJu Lee ◽  
Mohammad Hassan Baig ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cell Adhesion ◽  
3D Culture ◽  
Human Mesenchymal Stem Cells ◽  
Cell Types ◽  
Primary Cells ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Promote Cell Adhesion

In recent years, a major rise in the demand for biotherapeutic drugs has centered on enhancing the quality and efficacy of cell culture and developing new cell culture techniques. Here, we report fibronectin (FN) derived, novel peptides fibronectin-based intergrin binding peptide (FNIN)2 (18-mer) and FNIN3 (20-mer) which promote cell adhesion proliferation, and the differentiation of primary cells and stem cells. FNIN2 and 3 were designed based on the in silico interaction studies between FN and its receptors (integrin α5β1, αvβ3, and αIIbβ3). Analysis of the proliferation of seventeen-cell types showed that the effects of FNINs depend on their concentration and the existence of expressed integrins. Significant rhodamine-labeled FNIN2 fluorescence on the membranes of HeLa, HepG2, A498, and Du145 cells confirmed physical binding. Double coating with FNIN2 or 3 after polymerized dopamine (pDa) or polymerized tannic acid (pTA) precoating increased HBEpIC cell proliferation by 30–40 percent, suggesting FNINs potently affect primary cells. Furthermore, the proliferation of C2C12 myoblasts and human mesenchymal stem cells (MSCs) treated with FNINs was significantly increased in 2D/3D culture. FNINs also promoted MSC differentiation into osteoblasts. The results of this study offer a new approach to the production of core materials (e.g., cell culture medium components, scaffolds) for cell culture.

scholarly journals Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part II: Systems and Applications

Processes ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
Brigitte Altmann ◽  
Christoph Grün ◽  
Cordula Nies ◽  
Eric Gottwald
Keyword(s):  
Cell Culture ◽  
Spatial Distribution ◽  
3D Cell Culture ◽  
Research Area ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Cell Functions ◽  
Matrix Interactions ◽  
Definition Of ◽  
Extracellular Matrix Interactions

In this second part of our systematic review on the research area of 3D cell culture in micro-bioreactors we give a detailed description of the published work with regard to the existing micro-bioreactor types and their applications, and highlight important results gathered with the respective systems. As an interesting detail, we found that micro-bioreactors have already been used in SARS-CoV research prior to the SARS-CoV2 pandemic. As our literature research revealed a variety of 3D cell culture configurations in the examined bioreactor systems, we defined in review part one “complexity levels” by means of the corresponding 3D cell culture techniques applied in the systems. The definition of the complexity is thereby based on the knowledge that the spatial distribution of cell-extracellular matrix interactions and the spatial distribution of homologous and heterologous cell–cell contacts play an important role in modulating cell functions. Because at least one of these parameters can be assigned to the 3D cell culture techniques discussed in the present review, we structured the studies according to the complexity levels applied in the MBR systems.

scholarly journals Evaluation of Microbial Culture Techniques for the Isolation of Pythium Insidiosum from Equine Tissues

2002 ◽  
Vol 14 (4) ◽  
pp. 288-294 ◽  
Author(s):  
Amy M. Grooters ◽  
Amy Whittington ◽  
Mae K. Lopez ◽  
Michelle N. Boroughs ◽  
Alma F. Roy
Keyword(s):  
Room Temperature ◽  
Microbial Culture ◽  
Sample Type ◽  
Pythium Insidiosum ◽  
Selective Media ◽  
Culture Techniques ◽  
Media Type ◽  
Antibiotic Solution ◽  
And Storage ◽  
Storage Technique

The purpose of this study was to evaluate the effects of sample handling, storage, and culture techniques on the isolation of Pythium insidiosum from infected equine tissues. Tissue and kunker samples obtained immediately posteuthanasia from a horse with subcutaneous pythiosis were used to assess the effects of sample type (kunkers vs. tissues), media type (selective vs. nonselective), storage technique, and storage time on P. insidiosum isolation rate. Overall, isolation rates were higher from fresh kunkers (94.6%) and stored kunkers (76.4%) than from fresh tissues (8.3%) or stored tissues (4.6%). Isolation of P. insidiosum also occurred more often on antibiotic-containing media than on nonselective media for both fresh and stored samples. For samples that were stored for 1–3 days prior to culture, P. insidiosum isolation rates were highest for the following techniques: kunkers stored at room temperature and plated on selective media (100%), kunkers stored at 4 C and then plated on either nonselective (91.7%) or selective (95.8%) media, kunkers stored on cold packs and then plated on either nonselective (93.8%) or selective (100%) media, kunkers stored in ampicillin solution and plated on selective media (100%), and kunkers stored in ampicillin/gentocin solution and plated on selective media (87.5%). For samples stored for 4–5 days, P. insidiosum isolation rates were highest for kunkers stored at 4 C and then plated on either nonselective (81.3%) or selective (87.5%) media, kunkers stored in ampicillin solution and then plated on selective media (87.5%), and kunkers stored in ampicillin/gentocin solution and plated on selective media (87.5%). Results of this study suggest that optimal isolation rates of P. insidiosum from infected equine tissues are achieved by culturing fresh kunkers on selective media. For samples that cannot be processed immediately, acceptable handling techniques include storage at room temperature for up to 3 days, refrigeration for up to 5 days, shipping on cold packs, and storage in antibiotic solution, each combined with subsequent inoculation on selective media.

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