scholarly journals Profiling Inflammatory Genes and Signaling Pathways in Rheumatoid Synoviocytes for RA Light Therapy

10.5772/28158 ◽  
2012 ◽  
Author(s):  
Yasuko Shibata ◽  
Yoshimitsu Abiko
Keyword(s):  
Signaling Pathways ◽  
Light Therapy ◽  
Inflammatory Genes

scholarly journals Based on Proteomics Reveals the Anti-inflammatory Mechanism of Phillygenin by Inhibition NF-κB Pathway

2020 ◽  
Author(s):  
Mengting Zhou ◽  
Yunqiu Tang ◽  
Li Liao ◽  
Meichen Liu ◽  
Ying Deng ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Western Blot ◽  
Signaling Pathways ◽  
Inflammatory Factors ◽  
Transcriptional Level ◽  
Inflammatory Genes ◽  
Inflammatory Mechanism ◽  
Pathway Activation ◽  
Anti Inflammatory ◽  
Anti Inflammation

Abstract Background: Inflammation is a common pathological phenomenon when homeostasis is seriously disturbed. Phillygenin (PHI) is a lignin component isolated from Forsythiae Fructus, which showed a good anti-inflammation effect. However, the mechanisms of PHI on anti-inflammation have not yet been systematically elucidated.Methods: In the study, the lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model was established to investigate mechanisms of PHI on inflammation. The effect of PHI on LPS-induced IL-1β and PGE2 inflammatory factors was detected by ELISA, and the mRNA expression of IL-1β, IL-6 and TNF-α was detected by RT-qPCR. Proteomics studies the signaling pathways that may be affected by PHI. Molecular docking technology was used to study the possible targets of PHI on NF-kB pathway. Western blot was performed to detect progressive changes in protein expression.Results: The research showed that PHI significantly inhibited the robust increase of IL-1β and PGE2, and lowered the transcriptional level of inflammatory genes including IL-6, IL-1β and PGE2 in LPS-stimulated RAW264.7 cells. Proteomics results indicated that PHI was involved in the regulation of multiple signaling pathways. Molecular docking results indicated that PHI has an affinity for most proteins in NF-kB pathway. Western blot analysis showed that PHI inhibited LPS-induced NF-κB pathway activation. Conclusion: PHI inhibits the activation of NF-κB pathway, thereby inhibiting the expression of related inflammatory genes and the release of cytokines, thus showing remarkable anti-inflammatory effect.

scholarly journals Toll-Like Receptor Signaling Pathways—Therapeutic Opportunities

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Jiankun Zhu ◽  
Chandra Mohan
Keyword(s):  
Infectious Disease ◽  
Signaling Pathways ◽  
Transmembrane Proteins ◽  
Adaptor Proteins ◽  
Protective Role ◽  
Toll Like Receptor ◽  
Tlr Signaling ◽  
Inflammatory Genes ◽  
The Impact

Toll-like receptors (TLRs) are transmembrane proteins acting mainly as sensors of microbial components. Triggering TLRs results in increased expression of multiple inflammatory genes, which then play a protective role against infection. However, aberrant activation of TLR signaling has a significant impact on the onset of cancer, allergy, sepsis and autoimmunity. Various adaptor proteins, including MyD88, IRAKs, TIRAP, TRIF, and TRAM, are involved in specific TLR signaling pathways. This article reviews the role of these molecules in TLR signaling, and discusses the impact of this pathway on various disease scenarios. Given their important role in infectious and non-infectious disease settings, TLRs and their signaling pathways emerge as attractive targets for therapeutics.

A compendium of G-protein–coupled receptors and cyclic nucleotide regulation of adipose tissue metabolism and energy expenditure

2020 ◽  
Vol 134 (5) ◽  
pp. 473-512 ◽  
Author(s):  
Ryan P. Ceddia ◽  
Sheila Collins
Keyword(s):  
Adipose Tissue ◽  
Energy Expenditure ◽  
Signaling Pathways ◽  
G Protein ◽  
Uncoupling Protein ◽  
Beige Adipocytes ◽  
Nucleotide Regulation ◽  
Ucp1 Expression ◽  
Thermogenic Adipocytes ◽  
G Protein Coupled

Abstract With the ever-increasing burden of obesity and Type 2 diabetes, it is generally acknowledged that there remains a need for developing new therapeutics. One potential mechanism to combat obesity is to raise energy expenditure via increasing the amount of uncoupled respiration from the mitochondria-rich brown and beige adipocytes. With the recent appreciation of thermogenic adipocytes in humans, much effort is being made to elucidate the signaling pathways that regulate the browning of adipose tissue. In this review, we focus on the ligand–receptor signaling pathways that influence the cyclic nucleotides, cAMP and cGMP, in adipocytes. We chose to focus on G-protein–coupled receptor (GPCR), guanylyl cyclase and phosphodiesterase regulation of adipocytes because they are the targets of a large proportion of all currently available therapeutics. Furthermore, there is a large overlap in their signaling pathways, as signaling events that raise cAMP or cGMP generally increase adipocyte lipolysis and cause changes that are commonly referred to as browning: increasing mitochondrial biogenesis, uncoupling protein 1 (UCP1) expression and respiration.

Light Therapy Underwhelming for Acne

2007 ◽  
Vol 38 (2) ◽  
pp. 23
Author(s):  
DOUG BRUNK
Keyword(s):  
Light Therapy

Morning bright light therapy: a helpful tool for reducing comorbid symptoms of affective and behavioral dysregulation in juvenile depressed inpatients? A pilot trial

2017 ◽  
Vol 45 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Sarah Bogen ◽  
Tanja Legenbauer ◽  
Stephanie Gest ◽  
Martin Holtmann
Keyword(s):  
Pilot Trial ◽  
Secondary Analysis ◽  
Bright Light ◽  
Light Therapy ◽  
Bright Light Therapy ◽  
Circadian Phase ◽  
Behavioral Dysregulation ◽  
Sleep Parameters ◽  
Circadian Effect ◽  
Dysregulation Profile

Abstract. Objective: In recent years, bright light therapy (BLT) has been used to treat depression and to stabilize circadian rhythms. In this study we evaluated whether it is also helpful for comorbid symptoms of affective and behavioral dysregulation in depressive inpatients. Method: This article reports a secondary analysis comparing two subgroups of depressive participants with comorbid affective and behavioral dysregulation, captured with the dysregulation-profile of the Strengths and Difficulties Questionnaire (SDQ-DP; n = 16 vs. n = 11). Participants were randomly allocated to active BLT (10,000 lux) or control BLT (approx. 100 lux), and received 45 minutes of BLT for 2 weeks. SDQ-DP scores, sleep parameters, and circadian preference were assessed at baseline, after the intervention, and 3 weeks later. Results: No direct effects on SDQ-DP scores were observed. Sleep improved in both conditions. Only in the active BLT condition was a circadian phase advance found. Correlation and regression analyses indicated an indirect, circadian effect for improved SDQ-DP scores. Conclusions: The data of this pilot trial should be considered preliminary and merely descriptive. Further research is warranted.


Light Therapy as Treatment for Bulimia Nervosa

1994 ◽  
Author(s):  
R. W. Larn ◽  
◽  
E. M. Goldner ◽  
L. Solyom ◽  
R. A. Remick
Keyword(s):  
Bulimia Nervosa ◽  
Light Therapy
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