scholarly journals New Biomarkers for Cervical Cancer – Perspectives from the IGF System

10.5772/28007 ◽  
2012 ◽  
Author(s):  
Martha-Luca Serrano ◽  
Adriana Umaa-Prez ◽  
Diana J. ◽  
Myriam Snchez-Gmez
Keyword(s):  
Cervical Cancer ◽  
Igf System ◽  
New Biomarkers

scholarly journals Bioinformatics Prediction of miRNAs Targeting E6 and E7 Genes in Human Papillomavirus Types 16 and 18 in Cervical Cancer

2020 ◽  
Vol 23 (3) ◽  
pp. 374-385
Author(s):  
Tahere Azimi ◽  
◽  
Malihe Bagheri ◽  
Mahdi Pariyan ◽  
Behzad Khansarinejad ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Early Stage ◽  
Medical Sciences ◽  
Ethical Considerations ◽  
E7 Gene ◽  
Bioinformatics Databases ◽  
E6 And E7 ◽  
New Biomarkers

Background and Aim: Cervical Cancer (CC) is the third most common malignancy in the women, the main cause of which is human papillomavirus (HPV). Both E6 and E7 oncogenes of the virus play an important role in its tumorigenesis. Today, methods available for screening CC are not capable of detecting the disease at an early stage. Therefore, it is important to identify new biomarkers for early detection of this cancer. For this purpose, in the present study, miRNAs targeting the two oncogenes E6 and E7 of human papillomavirus (types 16 and 18) were studied in CC by bioinformatics. Methods & Materials: First, using the NCBI database, the E6 and E7 gene sequences were obtained for both human papillomavirus types 16 and 18. Then, using the miRBase and RNA22 bioinformatics databases, the most appropriate targeting miRNAs for these genes were selected. Ethical Considerations: This study was approved by Ethics Committee of Arak University of Medical Sciences. Results: Based on the P obtained from bioinformatics databases, miRNA including miR-92a-5p (P=7.51e-2), miR-195-3p (P=2.24e-1), miR-34a-5p (P=2.73e-1) and miR-155-5p (P=4.95e-2) were introduced for the two genes E6 and E7. Conclusion: Results from bioinformatics studies revealed that of the four miRNAs identified, miR-155-5p and miR-92a-5p are probably the targeting miRNAs specific for the E6 and E7 genes, respectively. Therefore, it seems that these miRNAs can be a suitable candidate for in vitro studies in CC patients.

scholarly journals Serum and tissue miRNAs: potential biomarkers for the diagnosis of cervical cancer

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Mahdieh Farzanehpour ◽  
Sayed-Hamidreza Mozhgani ◽  
Somayeh Jalilvand ◽  
Ebrahim Faghihloo ◽  
Setareh Akhavan ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Early Detection ◽  
Area Under The Curve ◽  
Normal Subjects ◽  
Expression Level ◽  
Serum Samples ◽  
Cancer Group ◽  
Potential Biomarker ◽  
New Biomarkers ◽  
Potential Biomarkers

Abstract Background Finding new biomarkers for the early detection of cervical cancer is an essential requirement in this field. In this study, we aimed to evaluate the expression level of potential biomarkers in progression of cervical cancer in patients with cervical cancer compared to normal subjects. Methods The expression levels of tissue and serum miRNAs, including miR-9, miR-192 and miR-205, were investigated in 36 normal, 18 precancer, and 18 cervical cancer samples using real-time polymerase chain reaction. Results The results showed the higher significant expressions of miR-9, miR-192 and miR-205 in the tissue of cancer samples than those in the normal samples. Moreover, the miR-192 and miR-205 expression were significantly increased in the cancer group in comparison with the precancer group. Examination of serum samples revealed the increase in the expression level in the cancer groups than in the normal samples, for miR-9, miR-192 and miR-205 and the expressions of miR-9, miR-192 and miR-205 were significantly up-regulated in the precancer group in comparison with the normal group. Also the expression of miR-205 was remarkably increased in the cancer group in comparison with the precancer group. The receiver operating characteristic (ROC) analyses showed the highest area under the curve value for miR-192. Conclusions Given the increased expression level of miR-192 in cancer and in precancerous tissue and serum compared with the normal tissue and serum validated by analysing the ROC curve, miR-192 can be used as potential biomarker for the early detection of cervical cancer.

Cervical Cancer Diagnosis: Insights into Biochemical Biomarkers and Imaging techniques

2020 ◽  
Vol 23 ◽  
Author(s):  
Fatemeh Tavakoli ◽  
Sara Sadat Khatami ◽  
Fatemeh Momeni ◽  
Javid Azadbakht ◽  
Faezeh Ghasemi
Keyword(s):  
Cervical Cancer ◽  
Cancer Therapy ◽  
Cancer Diagnosis ◽  
Treatment Process ◽  
Imaging Techniques ◽  
Multiple Pregnancies ◽  
Fast Detection ◽  
Novel Biomarkers ◽  
New Biomarkers ◽  
Cancer Multiple

: Cervical malignancy is known as one of the important cancers which is originated from cervix. This malignancy has been observed in women infected with papillomavirus who had regular oral contraceptives, multiple pregnancies and sexual relation. Early and fast cervical cancer diagnosis are known as two important aspects of cervical cancer therapy. Several investigations indicated that early and fast detection of cervical cancer could be associated with better treatment process and increasing survival rate of patients with this malignancy. Imaging techniques are very important diagnosis tools which could be employed for diagnosis and following responses to therapy in various stages of cervical cancer. Multiple lines of evidence indicated that utilization of imaging techniques are related to some limitations (i.e. high cost, and invasive effects). Hence, it seems that along with using imaging techniques, finding and developing new biomarkers could be useful in the diagnosis and treatment of subjects with cervical cancer. Taken together, many studies showed that a variety of biomarkers including, several proteins, mRNAs, microRNAs, exosomes and polymorphisms might be introduced as prognostic, diagnostic and therapeutic biomarkers in cervical cancer therapy. In this review article, we highlighted imaging techniques as well as novel biomarkers in diagnosis of cervical cancer.

scholarly journals Screening and Identification of New Biomarkers for the Control of Cervical Cancer

2020 ◽  
Author(s):  
Mari Uyeda ◽  
Mahendran Subramanian
Keyword(s):  
Cervical Cancer ◽  
P53 Gene ◽  
Diagnosis And Prognosis ◽  
Common Cancer ◽  
Epigenetic Events ◽  
Screening And Identification ◽  
Hpv Screening ◽  
Repeated Infections ◽  
New Biomarkers ◽  
Identification Techniques

Cervical cancer (CC) is the most common cancer in women worldwide and is almost always associated with repeated infections by human papillomavirus (HPV). Screening by traditional tests associated with biomarker identification techniques for low- or high-grade injuries, are becoming increasingly important in diagnosis and prognosis, avoiding countless deaths. This article explores existing literature on the main serum biomarkers and the identification of biomarkers associated with the oncogenesis of HPV expression in the identification of pre-cancerous lesions and of CC for an effective treatment, with reduction of recurrence, as well as we explain strategies for the development of biomarkers based on DNA, proteins and other markers. We also describe how markers of chromosomal instability host DNA, promoting hypo- or hypermethylation of DNA, as well as polymorphisms and epigenetic events in the p53 gene. Finally, we discuss changes in gene expression using cDNA microarray techniques and changes in the expression of proteins and markers identified through mass spectrometry (MS).

scholarly journals Potential new biomarkers for squamous carcinoma of the uterine cervix

ESMO Open ◽  
2018 ◽  
Vol 3 (4) ◽  
pp. e000352 ◽  
Author(s):  
Peter A van Dam ◽  
Christian Rolfo ◽  
Rossana Ruiz ◽  
Patrick Pauwels ◽  
Christophe Van Berckelaer ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cervical Screening ◽  
Invasive Cervical Cancer ◽  
Cancer Cell Lines ◽  
Invasive Cancer ◽  
Cervical Cancer Cell ◽  
Data Set ◽  
New Biomarkers

AimAn in silico pathway analysis was performed in an attempt to identify new biomarkers for cervical carcinoma.MethodsThree publicly available Affymetrix gene expression data sets (GSE5787, GSE7803, GSE9750) were retrieved, vouching for a total 9 cervical cancer cell lines, 39 normal cervical samples, 7 CIN3 samples and 111 cervical cancer samples. An Agilent data set (GSE7410; 5 normal cervical samples, 35 samples from invasive cervical cancer) was selected as a validation set. Predication analysis of microarrays was performed in the Affymetrix sets to identify cervical cancer biomarkers. We compared the lists of differentially expressed genes between normal and CIN3 samples on the one hand (n=1923) and between CIN3 and invasive cancer samples on the other hand (n=628).ResultsSeven probe sets were identified that were significantly overexpressed (at least 2 fold increase expression level, and false discovery rate <5%) in both CIN3 samples respective to normal samples and in cancer samples respective to CIN3 samples. From these, five probes sets could be validated in the Agilent data set (P<0.001) comparing the normal with the invasive cancer samples, corresponding to the genes DTL, HMGB3, KIF2C, NEK2 and RFC4. These genes were additionally overexpressed in cervical cancer cell lines respective to the cancer samples. The literature on these markers was reviewedConclusionNovel biomarkers in combination with primary human papilloma virus (HPV) testing may allow complete cervical screening by objective, non-morphological molecular methods, which may be particularly important in developing countries

scholarly journals Human papillomavirus-independent cervical cancer

2021 ◽  
pp. ijgc-2021-003014
Author(s):  
Andreina Fernandes ◽  
David Viveros-Carreño ◽  
Jorge Hoegl ◽  
Maira Ávila ◽  
Rene Pareja
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Clinical Outcomes ◽  
Kinase Inhibitor ◽  
Lymph Node Involvement ◽  
Diagnostic Techniques ◽  
Molecular Profile ◽  
Hpv Status ◽  
Molecular Profiles ◽  
New Biomarkers

Cervical cancer is the fourth most frequent cancer in women worldwide, representing nearly 8% of all female cancer deaths every year. The majority of cases of cervical cancer are caused by human papillomavirus (HPV); however, up to 5% of tumors are not associated with HPV-persistent infection and, moreover, the new WHO Female Genital Tumors classification subdivided cervical squamous and adenocarcinomas into HPV-associated and HPV-independent tumors. Based on this new information, the aim of this review is to provide an overview of HPV-independent cervical cancer, evaluating diagnostic techniques, molecular profiles, and clinical outcomes. The HPV-independent tumors are characterized by a differentiated molecular profile with lower proliferative activity, a p53 immunostaining, a decreased expression of cyclin-dependent kinase inhibitor proteins, such as p16, p14, and p27, and alterations in PTEN, p53, KRAS, CTNNB1, ARID1A, and ARID5B. HPV-independent tumors are associated with both adenocarcinomas and squamous histologic subtypes, with lymph node involvement in the early stages, more distant metastasis, and generally worse oncological outcomes. Thus far, no specific therapeutic strategies have been developed based on HPV status; however, with advancing knowledge of differences in the molecular profiles and possible targetable alterations, novel approaches may offer potential options in the near future. Investigators should report on clinical outcomes, evaluating the overall response rates to specific treatments, and consider new biomarkers to establish more accurate prognostics factors.

A combined ultrastructural and gene molecular research for the relationship between human uterine cervical carcinoma and human papillomavirus

1990 ◽  
Vol 48 (3) ◽  
pp. 204-205
Author(s):  
Kun Lee ◽  
Jingyi Si ◽  
Ricai Han ◽  
Wei Zhang ◽  
Bingbing Tan ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Dot Blot ◽  
Homologous Sequences ◽  
Normal Cervical Epithelium ◽  
The Relationship ◽  
Chronic Cervicitis

There are more supports for the view that human papillomavirus (HPV) infection might be an etiological factor in the development of cervical cancer when the association of persistent condylomata is considered. Biopsies from 318 cases with squamous cell carcinoma of uterine cervix, 48 with cervical and vulvar condylomata, 14 with cervical intraepithelial neoplasia (CIN), 34 with chronic cervicitis and 24 normal cervical epithelium were collected from 5 geographic regions of China with different cervical cancer mortalities. All specimens were prepared for Dot blot, Southern blot and in situ DNA-DNA hybridizations by using HPV-11, 16, 18 DNA labelled with 32P and 3H as probes to detect viral homologous sequences in samples. Among them, 32 cases with cervical cancer, 27 with condyloma and 10 normal cervical epitheliums were randomly chosen for comparative EM observation. The results showed that: 1), 192 out of 318 (60.4%) cases of cervical cancer were positive for HPV-16 DNA probe (Table I)

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